Label‐free quantitative mass spectrometry analysis of the circadian proteome of <i>Drosophila melanogaster lethal giant larvae</i> mutants reveals potential therapeutic effects of melatonin

Wong, Kum-Thong; Jayapalan, Jaime Jacqueline; Subramanian, Perumal; Ismail, Mohd Nazri; Abdul‐Rahman, Puteri Shafinaz

doi:10.1002/arch.22008

Cited by 2 publications

(2 citation statements)

References 84 publications

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“…Glutathione‐ S ‐transferase (GST) was shown to be suppressed by preparations of chili pepper that extended lifespan in D. melanogaster (Semaniuk et al, 2022a, 2022b). It was also shown that different isoenzymes of GST can be differentially regulated and affected by circadian rhythms (Wong et al, 2023).…”

Section: Discussionmentioning

confidence: 99%

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Alpha‐ketoglutarate supplementation in long‐lived Drosophila melanogaster: Impact on lifespan and metabolic responses

Demianchuk,

Lylyk,

Balatskiy

et al. 2024

Arch Insect Biochem Physiol

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Studies on antiaging remedies in insect models sometimes show discrepancies in results. These discrepancies could be explained by different responses of short‐ and long‐lived strains on the antiaging remedies. The purpose of the study was to test whether life‐prolonging effects of alpha‐ketoglutarate (AKG), observed in nematodes and fruit flies, would be reproduced in long‐lived Drosophila melanogaster flies. Lifespan was assayed in flies kept in demographic cages. Fecundity, proportion of flies capable of negative geotaxis, starvation resistance, time of heat coma onset, levels of triacyglycerols, body glucose, glycogen, activities of glutamate dehydrogenase, catalase, glutathione‐S‐transferase, hexokinase, phosphofructokinase, pyruvate kinase, lactate, and glutamate dehydrogenases were assessed. Dietary AKG did not affect fly lifespan on the diet with 5% yeast and 5% sucrose (5Y:5S) and on the diet with 9% yeast and 1% sucrose (9Y:1S), but increased lifespan on the low‐protein diet (1Y:9S). Twenty‐five‐day‐old female flies fed a 5Y:5S diet with 10 mM AKG for 3 weeks, did not differ from the control group (without AKG) in climbing activity, resistance to heat stress, and starvation. The levels of glucose and glycogen were unaffected but the levels of triacylglycerols were lower in AKG‐fed female flies. No differences in activities of glycolytic enzymes, NADPH‐producing enzymes, glutamate dehydrogenase, oxygen consumption, and levels of oxidative stress markers were observed between the control and AKG‐fed flies. However, AKG‐fed flies had lower activities of catalase and glutathione‐S‐transferase. These results suggest that potential antiaging remedies, such as AKG, may not extend lifespan in long‐living organisms despite influencing several metabolic parameters.

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Section: Discussionmentioning

confidence: 99%

“…It was also shown that different isoenzymes of GST can be differentially regulated and affected by circadian rhythms (Wong et al, 2023).…”

Section: The Influence Of Akg On the Activities Of Glycolytic Antioxi...mentioning

confidence: 99%

Alpha‐ketoglutarate supplementation in long‐lived Drosophila melanogaster: Impact on lifespan and metabolic responses

Demianchuk,

Lylyk,

Balatskiy

et al. 2024

Arch Insect Biochem Physiol

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Evolutionary formation of melatonin and vitamin D in early life forms: insects take centre stage

Kim,

Slominski,

Pyza

et al. 2024

Biological Reviews

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Melatonin, a product of tryptophan metabolism via serotonin, is a molecule with an indole backbone that is widely produced by bacteria, unicellular eukaryotic organisms, plants, fungi and all animal taxa. Aside from its role in the regulation of circadian rhythms, it has diverse biological actions including regulation of cytoprotective responses and other functions crucial for survival across different species. The latter properties are also shared by its metabolites including kynuric products generated by reactive oxygen species or phototransfomation induced by ultraviolet radiation. Vitamins D and related photoproducts originate from phototransformation of ∆5,7 sterols, of which 7‐dehydrocholesterol and ergosterol are examples. Their ∆5,7 bonds in the B ring absorb solar ultraviolet radiation [290–315 nm, ultraviolet B (UVB) radiation] resulting in B ring opening to produce previtamin D, also referred to as a secosteroid. Once formed, previtamin D can either undergo thermal‐induced isomerization to vitamin D or absorb UVB radiation to be transformed into photoproducts including lumisterol and tachysterol. Vitamin D, as well as the previtamin D photoproducts lumisterol and tachysterol, are hydroxylated by cyochrome P450 (CYP) enzymes to produce biologically active hydroxyderivatives. The best known of these is 1,25‐dihydroxyvitamin D (1,25(OH)2D) for which the major function in vertebrates is regulation of calcium and phosphorus metabolism. Herein we review data on melatonin production and metabolism and discuss their functions in insects. We discuss production of previtamin D and vitamin D, and their photoproducts in fungi, plants and insects, as well as mechanisms for their enzymatic activation and suggest possible biological functions for them in these groups of organisms. For the detection of these secosteroids and their precursors and photoderivatives, as well as melatonin metabolites, we focus on honey produced by bees and on body extracts of Drosophila melanogaster. Common biological functions for melatonin derivatives and secosteroids such as cytoprotective and photoprotective actions in insects are discussed. We provide hypotheses for the photoproduction of other secosteroids and of kynuric metabolites of melatonin, based on the known photobiology of ∆5,7 sterols and of the indole ring, respectively. We also offer possible mechanisms of actions for these unique molecules and summarise differences and similarities of melatoninergic and secosteroidogenic pathways in diverse organisms including insects.

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Label‐free quantitative mass spectrometry analysis of the circadian proteome of Drosophila melanogaster lethal giant larvae mutants reveals potential therapeutic effects of melatonin

Cited by 2 publications

References 84 publications

Alpha‐ketoglutarate supplementation in long‐lived Drosophila melanogaster: Impact on lifespan and metabolic responses

Alpha‐ketoglutarate supplementation in long‐lived Drosophila melanogaster: Impact on lifespan and metabolic responses

Evolutionary formation of melatonin and vitamin D in early life forms: insects take centre stage

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