Label-free quantitative proteomic analysis reveals potential biomarkers for early healing in cutaneous leishmaniasis

Montoya, Andrés; López, Manuel Carlos; Vélez, Iván Darío; Robledo, Sara M.

doi:10.7717/peerj.6228

Cited by 9 publications

(7 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The delay in disease development observed in the 10 5 group suggests that anti-leishmanial immune mechanisms were initially triggered but were not sufficient to overwhelm the parasite burden. Other lesion analysis tools, such as changes in the proteomic spectral signature recently described for the L. braziliensis CL in humans, will extend our understanding of skin lesion development ( 54 ). Herein an up-regulation of almost all investigated cytokines was observed during the chronic phase.…”

Section: Discussionmentioning

confidence: 99%

A Cytokine Network Balance Influences the Fate of Leishmania (Viannia) braziliensis Infection in a Cutaneous Leishmaniasis Hamster Model

et al. 2021

View full text Add to dashboard Cite

The golden hamster is a suitable model for studying cutaneous leishmaniasis (CL) due to Leishmania (Viannia) braziliensis. Immunopathological mechanisms are well established in the L. (L.) major-mouse model, in which IL-4 instructs a Th2 response towards progressive infection. In the present study, we evaluated the natural history of L. braziliensis infection from its first stages up to lesion establishment, with the aim of identifying immunological parameters associated with the disease outcome and parasitism fate. To this end, hamsters infected with 104, 105, or 106 promastigotes were monitored during the first hours (4h, 24h), early (15 days, 30 days) and late (50 days) post-infection (pi) phases. Cytokines, iNOS and arginase gene expression were quantified in the established lesions by reverse transcription-quantitative PCR. Compared to the 105 or 106 groups, 104 animals presented lower lesions sizes, less tissue damage, and lower IgG levels. Basal gene expression in normal skin was high for TGF-β, and intermediary for TNF, IL-6, and IL-4. At 4hpi, no cytokine induction was observed in the 104 group, while an upregulation of IL-6, IL-10, and IL-4 was observed in the 106 group. At 15dpi, lesion appearance was accompanied by an increased expression of all assessed cytokines, markedly in the 105 and 106 groups. Upregulation of all investigated cytokines was observed in the late phase, although less expressive in the 104 group. IFN-γ was the depending variable influencing tissue damage, while IL-6 was associated to parasite load. The network correlating gene expression and clinical and laboratorial parameters indicated inoculum-independent associations at 15 and 30dpi. A strong positive network correlation was observed in the 104 group, but not in the 105 or 106 groups. In conclusion, IL-4, IL-6, IL-10, and TGF-β are linked o L. braziliensis progression. However, a balanced cytokine network is the key for an immune response able to reduce the ongoing infection and reduce pathological damage.

show abstract

Section: Discussionmentioning

confidence: 99%

A Cytokine Network Balance Influences the Fate of Leishmania (Viannia) braziliensis Infection in a Cutaneous Leishmaniasis Hamster Model

et al. 2021

View full text Add to dashboard Cite

show abstract

“…of differentially regulated proteins Year References Study No. Up Down Proteins of Homo sapiens Infection based studies L. donovani 2DE; 2D-DIGE; MALDI-TOF/TOF MS/MS; Western blot; ELISA VL vs endemic control VL vs non-endemic control 38 10 2014 [ 35 ] 1 L. braziliensis 2DE-MALDI-TOF/TOF MS CL vs normal skin 9 4 2015 [ 38 ] 2 L. panamensis SDS-PAGE; LC ESI–MS/MS Start of treatment day vs 7th day of treatment 7 5 2019 [ 37 ] 3 Proteins of Mus Musculus Infection based studies L . amazonensis and L .…”

Section: Differentially Modulated Proteins In the Host After mentioning

confidence: 99%

“…Since glycoproteins are the preferred markers for diagnosing diseases [ 36 ], differentially expressed glycoproteins from the Bag et al [ 35 ] study can be used as prognostic and diagnostic markers for VL. Two differential protein expression studies [ 37 , 38 ], involving human subjects with CL, were reported. Da Silva Santos et al [ 38 ] compared the proteome of L. braziliensis infected skin lesion against normal skin biopsy and reported 13 DMP.…”

Section: Differentially Modulated Proteins In the Host After mentioning

confidence: 99%

“…The four down-regulated proteins include basal cell adhesion molecule, ankyrin repeat and LEM domain-containing protein, phosphodiesterase, and solute carrier family protein. In another recent study, Montoya et al [ 37 ] conducted a similar study comparing the proteome of L. panamensis infected human skin lesion against normal human skin and reported 12 DMP. The seven up-regulated proteins include keratin type I and II, lysosome-associated membrane glycoprotein, protein S100-A8 and A9, leukocyte elastase inhibitor, and copper-transporting ATPase.…”

Section: Differentially Modulated Proteins In the Host After mentioning

confidence: 99%

See 1 more Smart Citation

Differentially modulated proteins associated with Leishmaniasis—a systematic review of in-vivo and in-vitro studies

2020

View full text Add to dashboard Cite

High-throughput proteomic technologies are widely used for understanding the disease mechanism, drug-resistant mechanism, and to identify drug targets and markers for diagnostics. Studies with proteomics applications, relating to Leishmaniasis, are being constantly reported in the literature. However, from such studies, a readily accessible knowledge of differentially modulated proteins associated with Leishmaniasis is lacking. Hence, we performed a systematic review concerning differentially modulated proteins (DMP) in Leishmania as well as host infected with Leishmania from the published articles between the years 2000 and 2019. This review is classified into five different sections, namely, DMP in the host after Leishmania infection, DMP between different strains of Leishmania , DMP in drug-resistant Leishmania , DMP in Leishmania under stress, and DMP in different life stages of Leishmania. A lot of consensuses could be observed among the DMP in drug-resistant and stressed Leishmania . In addition to the review, a database was constructed with the data collected in this study (protein accession ID, protein name, gene name, host organism, experimental conditions, fold change, and regulatory data). A total of 2635 records are available in the database. We believe this review and the database will help the researcher in understanding the disease better and provide information for the targeted proteomics study related to Leishmaniasis. Database availability: http://ldepdb.biomedinformri.com/ . Electronic supplementary material The online version of this article (10.1007/s11033-020-05936-z) contains supplementary material, which is available to authorized users.

show abstract

“…Due to its straightforward method development for a wide variety of candidate analytes, LC-MS can often represent a more precise alternative to immunobinding assays and biomarker panel analyses in the field of targeted proteomics (Himmelsbach, 2012;Rauh, 2012). The use of unlabeled protein profiles to examine differentially expressed proteins in urine samples largely eliminates the variations and biases in replicate MS measurements (Atrih et al, 2014;Montoya et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Analysis of Spatiotemporal Urine Protein Dynamics to Identify New Biomarkers for Sepsis-Induced Acute Kidney Injury

Long

Chen

et al. 2020

Front. Physiol.

View full text Add to dashboard Cite

Acute kidney injury (AKI) is a frequent complication of sepsis and contributes to increased mortality. Discovery of reliable biomarkers could enable identification of individuals with high AKI risk as well as early AKI detection and AKI progression monitoring. However, the current methods are insensitive and non-specific. This study aimed to identify new biomarkers through label-free mass spectrometry (MS) analysis of a sepsis model induced by cecal ligation and puncture (CLP). Urine samples were collected from septic rats at 0, 3, 6, 12, 24, and 48 h. Protein isolated from urine was subjected to MS. Immunoregulatory biological processes, including immunoglobin production and wounding and defense responses, were upregulated at early time points. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses identified 77 significantly changed pathways. We further examined the consistently differentially expressed proteins to seek biomarkers that can be used for early diagnosis. Notably, the expression of PARK7 and CDH16 were changed in a continuous manner and related to the level of Scr in urine from patients. Therefore, PARK7 and CDH16 were confirmed to be novel biomarkers after validation in sepsis human patients. In summary, our study analyzed the proteomics of AKI at multiple time points, elucidated the related biological processes, and identified novel biomarkers for early diagnosis of sepsis-induced AKI, and our findings provide a theoretical basis for further research on the molecular mechanisms.

show abstract

Label-free quantitative proteomic analysis reveals potential biomarkers for early healing in cutaneous leishmaniasis

Cited by 9 publications

References 45 publications

A Cytokine Network Balance Influences the Fate of Leishmania (Viannia) braziliensis Infection in a Cutaneous Leishmaniasis Hamster Model

A Cytokine Network Balance Influences the Fate of Leishmania (Viannia) braziliensis Infection in a Cutaneous Leishmaniasis Hamster Model

Differentially modulated proteins associated with Leishmaniasis—a systematic review of in-vivo and in-vitro studies

Analysis of Spatiotemporal Urine Protein Dynamics to Identify New Biomarkers for Sepsis-Induced Acute Kidney Injury

Contact Info

Product

Resources

About