Label-free rapid electrochemical detection of DNA hybridization using ultrasensitive standalone CNT aerogel biosensor

Prakash, Jyoti; Dey, Anusree; Uppal, Sheetal; Alexander, Rajath; Kaushal, Amit; Misra, Hari S.; Dasgupta, Kinshuk

doi:10.1016/j.bios.2021.113480

Cited by 26 publications

(13 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Dau et al loaded Fe 3 O 4 onto graphene and increased the response current by a factor of 20 . A carbon nanostructure shows high stability, low cost, excellent conductivity, and facile surface functionalization and is regarded as a promising electrode material for electrochemical sensors, such as graphene, carbon nanotubes, active carbon, GDY, and so forth.…”

Section: Introductionmentioning

confidence: 99%

Ultrathin Graphdiyne/Graphene Heterostructure as a Robust Electrochemical Sensing Platform

Sun

Duan

et al. 2022

Anal. Chem.

View full text Add to dashboard Cite

Graphdiyne (GDY) has been considered as an appealing electrode material for electrochemical sensing because of its alkyne-rich structure and high degrees of π-conjugation, which shows great affinity to heavy metal ions and pollutant molecules via d−π and π–π interactions. However, the low surface area and poor conductivity of bulk GDY limit its electrochemical performance. Herein, a two-dimensional ultrathin GDY/graphene (GDY/G) nanostructure was synthesized and used as an electrode material for electrochemical sensing. Graphene plays the role of an epitaxy template for few-layered GDY growth and conductive layers. The formed few-layered GDY with a high surface area possesses abundant affinity sites toward heavy metal ions (Cd2+, Pb2+) and toxic molecules, for example, nitrobenzene and 4-nitrophenol, via d−π and π–π interactions, respectively. Moreover, hemin as a key part of the enzyme catalytic motif was immobilized on GDY/G via π–π interactions. The artificial enzyme mimic hemin/GDY/G-modified electrode exhibited promising ascorbic acid and uric acid detection performance with excellent sensitivity and selectivity, a good linear range, and reproducibility. More importantly, real sample detection and the feasibility of this electrochemical sensor as a wearable biosensor were demonstrated.

show abstract

Section: Introductionmentioning

confidence: 99%

Ultrathin Graphdiyne/Graphene Heterostructure as a Robust Electrochemical Sensing Platform

Sun

Duan

et al. 2022

Anal. Chem.

View full text Add to dashboard Cite

show abstract

“…The sensitivity of the sensor makes it possible to distinguish between fully complementary sequences as well as those containing a mismatch, while the detection limit against the fully complementary target DNA is 1 pM. The application area of this solution signaled by authors includes the detection of genetic markers of pathogens, as shown in Figure 15 [ 108 ].…”

Section: Role Of Carbon Nanomaterials and Their Composites In The Des...mentioning

confidence: 99%

Modern Electrochemical Biosensing Based on Nucleic Acids and Carbon Nanomaterials

Szymczyk

Ziółkowski

Malinowska

2023

Sensors

View full text Add to dashboard Cite

To meet the requirements of novel therapies, effective treatments should be supported by diagnostic tools characterized by appropriate analytical and working parameters. These are, in particular, fast and reliable responses that are proportional to analyte concentration, with low detection limits, high selectivity, cost-efficient construction, and portability, allowing for the development of point-of-care devices. Biosensors using nucleic acids as receptors has turned out to be an effective approach for meeting the abovementioned requirements. Careful design of the receptor layers will allow them to obtain DNA biosensors that are dedicated to almost any analyte, including ions, low and high molecular weight compounds, nucleic acids, proteins, and even whole cells. The impulse for the application of carbon nanomaterials in electrochemical DNA biosensors is rooted in the possibility to further influence their analytical parameters and adjust them to the chosen analysis. Such nanomaterials enable the lowering of the detection limit, the extension of the biosensor linear response, or the increase in selectivity. This is possible thanks to their high conductivity, large surface-to-area ratio, ease of chemical modification, and introduction of other nanomaterials, such as nanoparticles, into the carbon structures. This review discusses the recent advances on the design and application of carbon nanomaterials in electrochemical DNA biosensors that are dedicated especially to modern medical diagnostics.

show abstract

“…An ultrasensitive standalone CNT aerogel has been biosensor developed by Prakash et al, for the targeted detection of nucleic acids with a limit of detection of 1 pM with a turnaround time of less than 20 min. High sensitivity was obtained due to the exhibition of linear ohmic and near isotropic electrical properties by the multi-directional tenuous network of clustered CNTs embedded into the CNT aerogel electrode [ 68 ].…”

Section: Biomedical Applications Of Carbon Nanotubesmentioning

confidence: 99%

Carbon nanotubes in biomedical applications: current status, promises, and challenges

et al. 2022

View full text Add to dashboard Cite

In the past decade, there has been phenomenal progress in the field of nanomaterials, especially in the area of carbon nanotubes (CNTs). In this review, we have elucidated a contemporary synopsis of properties, synthesis, functionalization, toxicity, and several potential biomedical applications of CNTs. Researchers have reported remarkable mechanical, electronic, and physical properties of CNTs which makes their applications so versatile. Functionalization of CNTs has been valuable in modifying their properties, expanding their applications, and reducing their toxicity. In recent years, the use of CNTs in biomedical applications has grown exponentially as they are utilized in the field of drug delivery, tissue engineering, biosensors, bioimaging, and cancer treatment. CNTs can increase the lifespan of drugs in humans and facilitate their delivery directly to the targeted cells; they are also highly efficient biocompatible biosensors and bioimaging agents. CNTs have also shown great results in detecting the SARS COVID-19 virus and in the field of cancer treatment and tissue engineering which is substantially required looking at the present conditions. The concerns about CNTs include cytotoxicity faced in in vivo biomedical applications and its high manufacturing cost are discussed in the review. Supplementary Information The online version contains supplementary material available at 10.1007/s42823-022-00364-4.

show abstract

Label-free rapid electrochemical detection of DNA hybridization using ultrasensitive standalone CNT aerogel biosensor

Cited by 26 publications

References 28 publications

Ultrathin Graphdiyne/Graphene Heterostructure as a Robust Electrochemical Sensing Platform

Ultrathin Graphdiyne/Graphene Heterostructure as a Robust Electrochemical Sensing Platform

Modern Electrochemical Biosensing Based on Nucleic Acids and Carbon Nanomaterials

Carbon nanotubes in biomedical applications: current status, promises, and challenges

Contact Info

Product

Resources

About