Label-free real-time imaging of myelination in the<i>Xenopus laevis</i>tadpole by<i>in vivo</i>stimulated Raman scattering microscopy

Hu, Chunrui; Zhang, De‐Long; Slipchenko, Mikhail N.; Cheng, Ji‐Xin; Hu, Bing

doi:10.1117/1.jbo.19.8.086005

Cited by 24 publications

(15 citation statements)

References 62 publications

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“…For low laser powers applied for biological samples, the Raman gain or loss differs approximately four orders of magnitude from the laser intensity (97); therefore, the enhancing beams are modulated and the signal is measured using the respective modulation frequency in a lock-in detection scheme. Several SRS studies have been performed (98), including investigations of cancer development in various tissue types (99)(100)(101)(102), lipid metabolism (103), drug penetration experiments (104,105), as well as nervous system-related (106) and cardiovascular diseases (107).…”

Section: Raman-based Microscopymentioning

confidence: 99%

Multimodal Imaging Spectroscopy of Tissue

Vogler

Heuke

Bocklitz

et al. 2015

Annual Rev. Anal. Chem.

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Advanced optical imaging technologies have experienced increased visibility in medical research, as they allow for a label-free and nondestructive investigation of tissue in either an excised state or living organisms. In addition to a multitude of ex vivo studies proving the applicability of these optical imaging approaches, a transfer of various modalities toward in vivo diagnosis is currently in progress as well. Furthermore, combining optical imaging techniques, referred to as multimodal imaging, allows for an improved diagnostic reliability due to the complementary nature of retrieved information. In this review, we provide a summary of ongoing multifold efforts in multimodal tissue imaging and focus in particular on in vivo applications for medical diagnosis. We also discuss the advantages and potential limitations of the imaging methods and outline opportunities for future developments.

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Section: Raman-based Microscopymentioning

confidence: 99%

Multimodal Imaging Spectroscopy of Tissue

Vogler

Heuke

Bocklitz

et al. 2015

Annual Rev. Anal. Chem.

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“…Additionally, myelination in tadpoles has been followed in vivo using SRS. 144 Because of the absence of lipids in the nuclei of cells, they are often used to provide cellular contrast. The usefulness of lipids as a contrast mechanism and the ability to show localization and dynamics of specific lipid species have been shown repeatedly.…”

Section: Bulk Ensembles To Single Moleculesmentioning

confidence: 99%

Stimulated Raman Scattering: From Bulk to Nano

2016

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Stimulated Raman scattering (SRS) describes a family of techniques first discovered and developed in the 1960s. Whereas the nascent history of the technique is parallel to that of laser light sources, recent advances have spurred a resurgence in its use and development that has spanned across scientific fields and spatial scales. SRS is a nonlinear technique that probes the same vibrational modes of molecules that are seen in spontaneous Raman scattering. While spontaneous Raman scattering is an incoherent technique, SRS is a coherent process, and this fact provides several advantages over conventional Raman techniques, among which are much stronger signals and the ability to time-resolve the vibrational motions. Technological improvements in pulse generation and detection strategies have allowed SRS to probe increasingly smaller volumes and shorter time scales. This has enabled SRS research to move from its original domain, of probing bulk media, to imaging biological tissues and single cells at the micro scale, and, ultimately, to characterizing samples with subdiffraction resolution at the nanoscale. In this Review, we give an overview of the history of the technique, outline its basic properties, and present historical and current uses at multiple length scales to underline the utility of SRS to the molecular sciences.

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“…For example, Hu et al (120) performed in vivo SRS imaging of single neurons in the tectum (Figure 3 c , left panel) and myelin structures in the spinal cord (Figure 3 c , right panel) in early-stage tadpoles. C. elegans has been extensively studied using SRS microscopy (42, 95, 109, 121).…”

Section: Applications Using Crs Microscopymentioning

confidence: 99%

“…Abbreviations: Daf-2 , L2: daf-2 mutant C. elegans at L2 stage; LROs: lysosome-related organelles; SRS: stimulated Raman scattering; WT N2, L2: wild-type N2 C. elegans at the L2 stage. Panels a , b , c , and d are adapted with permission from References 78, 115, 120, and 121, respectively.…”

Section: Figurementioning

confidence: 99%

Coherent Raman Scattering Microscopy in Biology and Medicine

Zhang

Zhang²,

Cheng³

2015

Annu. Rev. Biomed. Eng.

Self Cite

181

146

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Advancements in coherent Raman scattering (CRS) microscopy have enabled label-free visualization and analysis of functional, endogenous biomolecules in living systems. When compared with spontaneous Raman microscopy, a key advantage of CRS microscopy is the dramatic improvement in imaging speed, which gives rise to real-time vibrational imaging of live biological samples. Using molecular vibrational signatures, recently developed hyperspectral CRS microscopy has improved the readout of chemical information available from CRS images. In this article, we review recent achievements in CRS microscopy, focusing on the theory of the CRS signal-to-noise ratio, imaging speed, technical developments, and applications of CRS imaging in bioscience and clinical settings. In addition, we present possible future directions that the use of this technology may take.

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Label-free real-time imaging of myelination in theXenopus laevistadpole byin vivostimulated Raman scattering microscopy

Cited by 24 publications

References 62 publications

Multimodal Imaging Spectroscopy of Tissue

Multimodal Imaging Spectroscopy of Tissue

Stimulated Raman Scattering: From Bulk to Nano

Coherent Raman Scattering Microscopy in Biology and Medicine

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