Label-free scattering image-spectro-microscopy for single cell and extracellular vesicle analysis

Eltigani, Faihaa Mohammed; Zhang, Xiaoyu; Liu, Qiao; Liu, Min; Peng, Jun; Su, Xuantao

doi:10.1016/j.snb.2023.134572

Cited by 7 publications

(1 citation statement)

References 48 publications

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“…Extracellular vesicles (EVs) are essential in understanding various physiological and pathological conditions, which have received intensive attention recently. , For example, metastasis is the leading cause of death in cancer patients, where EVs of cancerous cells can create a path into the bloodstream and allow cancer migration . It is reported that EVs with an average size of approximately 100 nm or less are generally defined as small extracellular vesicles (sEVs), , which fall in the category of nanoparticles (NPs).…”

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confidence: 99%

Label-Free Light Scattering Imaging with Purified Brownian Motion Differentiates Small Extracellular Vesicles in Cell Microenvironments

Mohamed,

Wang,

Liu

et al. 2024

Anal. Chem.

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Small extracellular vesicles (sEVs) are heterogeneous biological nanoparticles (NPs) with wide biomedicine applications. Tracking individual nanoscale sEVs can reveal information that conventional microscopic methods may lack, especially in cellular microenvironments. This usually requires biolabeling to identify single sEVs. Here, we developed a light scattering imaging method based on dark-field technology for label-free nanoparticle diffusion analysis (NDA). Compared with nanoparticle tracking analysis (NTA), our method was shown to determine the diffusion probabilities of a single NP. It was demonstrated that accurate size determination of NPs of 41 and 120 nm in diameter is achieved by purified Brownian motion (pBM), without or within the cell microenvironments. Our pBM method was also shown to obtain a consistent size estimation of the normal and cancerous plasma-derived sEVs without and within cell microenvironments, while cancerous plasma-derived sEVs are statistically smaller than normal ones. Moreover, we showed that the velocity and diffusion coefficient are key parameters for determining the diffusion types of the NPs and sEVs in a cancerous cell microenvironment. Our light scattering-based NDA and pBM methods can be used for size determination of NPs, even in cell microenvironments, and also provide a tool that may be used to analyze sEVs for many biomedical applications.

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