Label-free Si quantum dots as photoluminescence probes for glucose detection

Yi, Yinhui; Deng, Junhong; Zhang, Youyu; Li, Haitao; Yao, Shouzhuo

doi:10.1039/c2cc36282a

Cited by 128 publications

(83 citation statements)

References 30 publications

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“…236,237 As a result, many QD-based glucose sensors feature glucose oxidase appended to a QD surface as soluble optical sensors, 238–244 solid state optical sensors, 245–247 or solid state (photo)electro-chemical/electrochemiluminescent sensors. 248–254 In these sensors, H 2 O 2 , which is generated during enzyme turnover, quenches QD luminescence in optical sensors, or is detected electrochemically to quantify the amount of glucose in the sample.…”

Section: Qd-based Glucose Chemosensorsmentioning

confidence: 99%

Metabolic Tumor Profiling with pH, Oxygen, and Glucose Chemosensors on a Quantum Dot Scaffold

et al. 2013

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Synopsis Acidity, hypoxia and glucose levels characterize the tumor microenvironment rendering pH, pO2 and pGlucose, respectively, important indicators of tumor health. To this end, understanding how these parameters change can be a powerful tool for the development of novel and effective therapeutics. We have designed optical chemosensors that feature a quantum dot and an analyte-responsive dye. These non-invasive chemosensors permit pH, oxygen, and glucose to be monitored dynamically within the tumor microenvironment by using multiphoton imaging.

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Section: Qd-based Glucose Chemosensorsmentioning

confidence: 99%

Metabolic Tumor Profiling with pH, Oxygen, and Glucose Chemosensors on a Quantum Dot Scaffold

et al. 2013

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“…En bondad de la fuerte y estable fluorescencia de los nanomateriales de silicio, se han diseñado distintos sensores para la detección de especies químicas y biológicas como glucosa [74], agroquímicos [75], explosivos nitroaromáticos [76]. Otra aplicación importante se logró al dopar nanorods de silicio (NRSis) con europio (Eu@NRSis), para medir las fluctuaciones en el pH intracelular, basándose en detección radiométrica sin necesidad de utilizar más sustancias químicas [77].…”

Section: Biosensoresunclassified

Nanoestructuras De Silicio en Biomedicina Y Biotecnología

Rios¹,

Vega-Baudrit

Villegas³

et al. 2020

Momento

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Silicon nanostructures have properties that make them appealing for applications in biomedicine and biotechnology. The fabrication and characterization of silicon nanostructures are currently gaining a lot of attention and they can include nano-wires, whiskers, pillars, tubes, cones, particles, etc. There are several methods to assist the generation of these nanostructures like laser ablation, thermal evaporation decomposition, and chemical vapor deposition, among others. We consider they are highly potential tools for the improving of diagnosis techniques and the treatment of important biological conditions. The objective of this review is to summarize the main aspects of the physicochemical design of silicon nanomaterials, in addition to toxicological considerations regarding biocompatibility, biodistribution and cell dynamics. Finally, we emphasis on important biomedical applications, such as gene therapy, drug delivery, imaging and sensoring.

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“…So far, glucose oxidase was used to construct electrochemical [19,20], fluorescence [21,22] and colorimetric [23] sensors for glucose directly. On the other hand, glucose oxidase was conjugated to biorecognition molecule and acted as amplifying label, which was used to establish electrochemical Electronic supplementary material The online version of this article (doi:10.1007/s00604-015-1515-7) contains supplementary material, which is available to authorized users.…”

Section: Introductionmentioning

confidence: 99%

Aptamer-based fluorescent solid-phase thrombin assay using a silver-coated glass substrate and signal amplification by glucose oxidase

Ling

2015

Microchim Acta

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We describe an aptamer-based solid-state biosensor for the fluorometric determination of thrombin. The surface of silver-coated glass was modified with the thrombin-binding aptamer 1 (TBA 1) of the sequence 5′-HS-TTT TTT TTT TTT TTT GGT TGG TGT GGT TGG-3′. A second (and biotinylated) thrombin -binding aptamer (TBA 2) with the sequence 5′-biotin-AGT CCG TGG TAG GGC AGG TTG GGG TGA CT-3′ was applied as the signaling aptamer. Following binding of thrombin by TBA 1 on the surface, TBA 2 is added and then binds to the thrombin on the surface of the silver-coated glass to form the thrombin-aptamer complex. The biotin groups on TBA 2 are then coated with streptavidin, and biotin-labeled glucose oxidase (biotinGOx) is added to bind to streptavidin. The quantity of GOx immobilized in this way is directly related to the quantity of thrombin bound on the surface. Following cleavage of the aptamer with DNase I, glucose is added and oxidized by GOx to yield H 2 O 2 . Horseradish peroxidase is added and causes the oxidation of 3-p-hydroxyphenylpropanoic acid to yield a fluorescent product. The intensity of the blue fluorescence is directly related to the thrombin concentration in the 300 pM to 6500 pM range, and the detection limit is as low as 82 pM. The assay has good selectivity and practicability.

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Label-free Si quantum dots as photoluminescence probes for glucose detection

Cited by 128 publications

References 30 publications

Metabolic Tumor Profiling with pH, Oxygen, and Glucose Chemosensors on a Quantum Dot Scaffold

Metabolic Tumor Profiling with pH, Oxygen, and Glucose Chemosensors on a Quantum Dot Scaffold

Nanoestructuras De Silicio en Biomedicina Y Biotecnología

Aptamer-based fluorescent solid-phase thrombin assay using a silver-coated glass substrate and signal amplification by glucose oxidase

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