Label-free SPR detection of gluten peptides in urine for non-invasive celiac disease follow-up

Soler, María; Estévez, M.‐Carmen; Moreno, María de Lourdes; Cebolla, Ángel; Lechuga, Laura M.

doi:10.1016/j.bios.2015.11.097

Cited by 68 publications

(50 citation statements)

References 40 publications

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“…The SPR gold chip was first modified with a mixed monolayer of 11-mercaptoundecanoic acid (MUA) and 11-mercaptoundecanol (MUD). 33 Both the Tau specific DNA aptamer and control sequences were covalently coupled to MUA via a 5' end NH2 modification and EDC/NHSS linking chemistry. Tau specific adsorption measurements were first performed in buffer to establish the binding affinity of the DNA aptamer before progressing to measurements in plasma.…”

Section: Spr Detection Of Tau Proteinmentioning

confidence: 99%

Femtomolar Detection of Tau Proteins in Undiluted Plasma Using Surface Plasmon Resonance

2016

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The ability to directly detect Tau protein and other neurodegenerative biomarkers in human plasma at clinically relevant concentrations continues to be a significant hurdle for the establishment of diagnostic tests for Alzheimer's disease (AD). In this article, we introduce a new DNA aptamer/antibody sandwich assay pairing and apply it for the detection of human Tau 381 in undiluted plasma at concentrations as low as 10 fM. This was achieved on a multichannel surface plasmon resonance (SPR) platform with the challenge of working in plasma overcome through the development of a tailored mixed monolayer surface chemistry. In addition, a robust methodology was developed involving various same chip control measurements on reference channels to which the detection signal was normalized. Comparative measurements in plasma between SPR and enzyme-linked immunosorbent assay (ELISA) measurements were also performed to highlight both the 1000-fold performance enhancement of SPR and the ability to measure both spiked and native concentrations that are not achievable with ELISA.

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Section: Spr Detection Of Tau Proteinmentioning

confidence: 99%

Femtomolar Detection of Tau Proteins in Undiluted Plasma Using Surface Plasmon Resonance

2016

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“…These assays may be a practical method to assess dietary adherence in coeliac patients . GIP are resistant to gastrointestinal digestion and account for immunogenic reactions in T cells of patients with coeliac disease . Unlike traditional methods to monitor GFD adherence which only evaluate the consequences of GFD transgressions, this non‐invasive method enables a direct and quantitative assessment of gluten exposure.…”

Section: Introductionmentioning

confidence: 99%

Prospective longitudinal study: use of faecal gluten immunogenic peptides to monitor children diagnosed with coeliac disease during transition to a gluten‐free diet

Comino¹,

Segura²,

Ortigosa³

et al. 2019

Aliment Pharmacol Ther

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Summary Background Treatment for coeliac disease is a lifelong strict gluten‐free diet. Although guidelines recommend regular follow‐up with dietary interviews and coeliac serology, these methods may be inaccurate. Aim To evaluate the usefulness of faecal gluten immunogenic peptides to support the diagnosis and to determine the adherence to the gluten‐free diet in coeliac children. Methods Multicentre prospective observational study including 64 coeliac children. Faecal gluten peptides, and tissue transglutaminase and deamidated gliadin peptide antibodies were analyzed at diagnosis, and 6, 12 and 24 months thereafter. Gluten consumption was estimated from gluten peptide levels. Results Most children (97%) had detectable gluten peptides at diagnosis. On a gluten‐free diet, the rate of gluten peptides increased from 13% at 6 months to 25% at 24 months. Mean estimated gluten exposure dropped from 5543 mg/d at diagnosis to 144 mg/d at 6 months, then increased to 606 mg/d by 24 months. In contrast, deamidated gliadin peptide antibodies normalised and only 20% had elevated tissue transglutaminase antibody by 24 months. The elevation of tissue transglutaminase antibody was more prolonged in patients with detectable gluten peptides (P < 0.05). Nevertheless, absolute levels of tissue transglutaminase antibody had low sensitivity to identify patients with detectable gluten peptides (P > 0.1). Dietitian assessment was only moderately correlated with gluten peptide detection (κ = 0.5). Conclusions Faecal gluten peptides testing may guide treatment of coeliac disease prior to diagnosis and during the assessment diet adherence. Further studies could determine if early identification of gluten exposure reduces the need for expensive/invasive investigations for non‐responsive coeliac disease. ClinicalTrials.gov Number: NCT02711397.

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“…In regard to the real-time monitoring tools, Localized Surface Plasmon Resonance (LSPR) is a highly sensitive and cost-effective optical technique for label-free biosensing, widely demonstrated in the literature, for example, for the detection of gluten peptides or tumor-associated autoantibodies. 19,20 Owing to the high sensitivity of LSPR to local refractive index changes near the metal surface, the LSPR change can be used to study the cell behaviour and, thereby, to study the cell integrin-ECM linkage. On other hand, plasmonic nanostructures can be fabricated over polymer nanostructures to provide them height and flexibility.…”

Section: Introductionmentioning

confidence: 99%

Building of a flexible microfluidic plasmo-nanomechanical biosensor for live cell analysis

Solis-Tinoco

Márquez

Quesada‐López

et al. 2019

Sensors and Actuators B: Chemical

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Biosensor devices can constitute an advanced tool for monitoring and study complex dynamic biolog ical processes, as for example cellular adhesion. Cellular adhesion is a multipart process with crucial implications in physiology (i.e. immune response, tissue nature, architecture maintenance, or behavior and expansion of tumor cells). This work focuses on offering a controlled methodology in order to fabricate a flexible plasmo-nanomechanical biosensor placed within a microfluidic channel as a new tool for future cell adhesion studies. We designed, fabricated, and optically and mechanically characterized this novel optical biosensor. As a proof-of-concept of its functionality, the biosensor was employed to observe fibroblasts adhesion in a cell culture. The device is configured by an hexagonal array of flexible rigid/soft polymeric nanopillars capped with plasmonic gold nanodisks integrated inside a microfluidic channel. The fabrication employs low-cost and large-scale replica molding techniques using two different polymers materials (EPOTECK OG142 and 310M). By using those materials the spring constant of the polymer nanopillars (k) can be fabricated from 1.19E-02 [N/m] to 5.35E +00 [N/m] indicating different mechanical sensitivities to shear stress. Therefore, the biosensor has the feasibility to mimic soft and rigid tissues important for the description of cellular nanoscale behaviours. The biosensor exhibits a suitable bulk sensitivity of 164 nm or 206 nm/refractive index unit respectively, depending on the base material. The range of calculated forces goes from ≈1.98 nN to ≈.942 µN. This supports that the plasmo-nanomechanical biosensors could be employed as novel tool to study living cells behavior.

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Label-free SPR detection of gluten peptides in urine for non-invasive celiac disease follow-up

Abstract: Label-free SPR detection of gluten peptides in urine for non-invasive celiac disease follow-up. Biosensors and Bioelectronics, (2016). 79.

Cited by 68 publications

References 40 publications

Femtomolar Detection of Tau Proteins in Undiluted Plasma Using Surface Plasmon Resonance

Femtomolar Detection of Tau Proteins in Undiluted Plasma Using Surface Plasmon Resonance

Prospective longitudinal study: use of faecal gluten immunogenic peptides to monitor children diagnosed with coeliac disease during transition to a gluten‐free diet

Building of a flexible microfluidic plasmo-nanomechanical biosensor for live cell analysis

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