Label‐free stimulated Raman scattering imaging reveals silicone breast implant material in tissue

Abstract

Millions of women worldwide have silicone breast implants. It has been reported that implant failure occurs in approximately a tenth of patients within 10 years, and the consequences of dissemination of silicone debris are poorly understood. Currently, silicone detection in histopathological slides is based on morphological features as no specific immunohistochemical technique is available. Here, we show the feasibility and sensitivity of stimulated Raman scattering (SRS) imaging to specifically detect silicone material in stained histopathological slides, without additional sample treatment. Histology slides of four periprosthetic capsules from different implant types were obtained after explantation, as well as an enlarged axillary lymph node from a patient with a ruptured implant.Abbreviations: CC, connected component; OPO, optical parametric oscillator; PDMS, polydimethylsiloxane; PU, polyurethane; RMSE, root mean square error; ROI, region of interest; SRS, stimulated Raman scattering.Ludo van Haasterecht and Liron Zada contributed equally to this work.

Multimodal, label-free fluorescence and Raman imaging of amyloid deposits in snap-frozen Alzheimer’s disease human brain tissue

“…Stimulated Raman scattering microscopy. For SRS measurements, we used an in-house built picosecond system as described in more detail in previous works 25,92,93 . In short, the Stokes beam (λ = 1064 nm, 80 MHz repetition rate, power 25 mW at the sample plane) was amplitude modulated at 3.6 MHz.…”

“…We used auto-fluorescence microscopy, followed by spontaneous Raman spectroscopy using a 532 nm excitation source to examine the unstained plaque tissue sections. Afterward, we recorded stimulated Raman scattering (SRS) 24,25 images of the same tissue areas. To the best of our knowledge, it is the first time that various imaging and spectroscopic modalities were used consecutively on the same native AD tissue section.…”

Recent advances in the use of stimulated Raman scattering in histopathology

“…Haasterecht et al, developed an SRS technique to specifically detect micrometer (≥2 μm) sized polydimethylsiloxane (PDMS; the filling of modern implants) fragments that have a C-H stretch vibrational frequency at 2905 cm −1 which is distinct from that of the major tissue constituents. 42 Histopathological samples collected following removal of failed implants identified large aggregates of PDMS. In one case, localization within vacuoles was detected in an enlarged axillary lymph node from a patient with a ruptured implant.…”

Endoscopic Optical Imaging Technologies and Devices for Medical Purposes: State of the Art

“…The scattered signal is higher than that of the spontaneous Stokes shift [10]. A large number of CARS microscopy probes have been designed to image cancer cells [33,34], as well as SRS microscopy systems to image the distribution of molecules, intracellular particles, proteins, and tumor cells [35,36]. A list of CARS and SRS microscopy devices is included in.…”