Label-Free Surface-Enhanced Raman Spectroscopy Detection of DNA with Single-Base Sensitivity

Xu, Lijia; Lei, Zhichao; Li, Jiuxing; Zong, Cheng; Yang, Chaoyong; Ren, Bin

doi:10.1021/jacs.5b01426

Cited by 398 publications

(354 citation statements)

References 47 publications

Supporting

Mentioning

351

Contrasting

Order By: Relevance

“…Surface-enhanced Raman scattering (SERS) possesses several attractive properties, such as ultrahigh sensitivity, high speed, comparatively low cost, and multiplexing ability and portability [69][70][71], which enable SERS to be widely used for sensitive detection of chemical and biological agents [72,73]. Since Holt and Cotton first reported the SERS spectrum of bacteria, the identification and detection of microorganism by SERS have attracted high interest recently due to the spectroscopic fingerprint and nondestructive data acquisition in aqueous environment [74].…”

Section: Optical Strategiesmentioning

confidence: 99%

Current and Emerging Technologies for Rapid Detection of Pathogens

Zeng¹,

Wang²,

Hu³

2018

Biosensing Technologies for the Detection of Pathogens - A Prospective Way for Rapid Analysis

View full text Add to dashboard Cite

Foodborne diseases, caused by pathogenic bacteria, have become an important social issue in the field of food safety. It presents a widespread and growing threat to human health in both developed and developing countries. As such, techniques for the detection of foodborne pathogens and waterborne pathogens are urgently needed to prevent the occurrence of human foodborne infections. Although traditional culture-based bacterial isolation and identification are the "gold standard" methods with high preciseness, their drawbacks in time-consuming are inadequate for rapid detection of pathogen to reduce foodborne disease occurrence. Fortunately, with the development of biotechnologies and nanotechnologies, various kinds of new technologies for rapid detection of pathogens have been developed so far, such as nucleic acid-based methods, antibody-based methods, and aptamer-based assays. In this chapter, we summarized the principles and the application of some recent rapid detection technologies for pathogenic bacteria. Moreover, the advantages and disadvantages of the established and emerging rapid detection methods are addressed here.

show abstract

Section: Optical Strategiesmentioning

confidence: 99%

Current and Emerging Technologies for Rapid Detection of Pathogens

Zeng¹,

Wang²,

Hu³

2018

Biosensing Technologies for the Detection of Pathogens - A Prospective Way for Rapid Analysis

View full text Add to dashboard Cite

show abstract

“…Notes: (A) UV-Vis absorption spectra of gNrs (solid lines) and gO@gNrs (dotted lines) with different aspect ratios. (B) raman spectra obtained from the gO@gNrs with different lsPr peaks (680 nm [1], 700 nm [2], 760 nm [3], 780 nm [4], and 810 nm [5]). Inset shows the sers intensities of 1,207 cm -1 .…”

Section: Photothermal Effects and Real-time Monitoring Of Nir Sers Prmentioning

confidence: 99%

“…[1][2][3][4][5][6] The SERS substrate can also be designed to serve as a sensitive Raman probe for bioimaging under in vitro and in vivo environments. [7][8][9][10][11] Despite the remarkable progress in SERS, there are many bottlenecks hindering its development.…”

Section: Introductionmentioning

confidence: 99%

Development of graphene oxide-wrapped gold nanorods as robust nanoplatform for ultrafast near-infrared SERS bioimaging

Qiu¹,

You²,

Chen³

et al. 2017

IJN

View full text Add to dashboard Cite

The rapid development of near-infrared surface-enhanced Raman scattering (NIR SERS) imaging technology has attracted strong interest from scientists and clinicians due to its narrow spectral bandwidth, low background interference, and deep imaging depth. In this report, the graphene oxide (GO)-wrapped gold nanorods (GO@GNRs) were developed as a smart and robust nanoplatform for ultrafast NIR SERS bioimaging. The fabricated GO@ GNRs could efficiently load various NIR probes, and the in vitro evaluation indicated that the nanoplatform could exhibit a higher NIR SERS activity in comparison with traditional gold nanostructures. The GOs were prepared by directly pyrolyzing citric acid for greater convenience, and GO@GNRs were fabricated via a facile synthesis strategy. Higher NIR SERS activity, facile synthesis method, excellent biocompatibility, and superb stability make the GO@GNRs/probe complex promising nanoprobes for NIR SERS-based bioimaging applications.

show abstract

“…In order to obtain greater sensitivity and signal enhancement, a variety of nanoparticles have been assembled into solid substrates (such as graphite, silicon, and ITO glass) by template method, self-assembly, nanosphere lithography, and electrochemical deposition. Recently, SERS has been used for biosensing virus, bacteria, proteins, and nucleic acid [23,24]. Khlebtsov et al fabricated centimeterscale gold nanoisland films as reproducible SERS substrates for highly sensitive detection of the thiram fungicide in apple peels [25].…”

Section: Introductionmentioning

confidence: 99%

Rapid Detection and Identification of miRNAs by Surface-Enhanced Raman Spectroscopy Using Hollow Au Nanoflowers Substrates

Cao

Bao

Shan

et al. 2017

Journal of Nanomaterials

View full text Add to dashboard Cite

MicroRNAs (miRNAs) are recognized as regulators of gene expression during the biological processes of cells as well as biomarkers of many diseases. Development of rapid and sensitive miRNA profiling methods is crucial for evaluating the pattern of miRNA expression related to normal and diseased states. This work presents a novel hollow Au nanoflowers (HAuNFs) substrate for rapid detection and identification of miRNAs by surface-enhanced Raman scattering (SERS) spectroscopy. We synthesized the HAuNFs by a seed-mediated growth approach. Then, HAuNFs substrates were fabricated by depositing HAuNFs onto the surfaces of (3-aminopropyl)triethoxysilane-(APTES-) functionalized ITO glass. The result demonstrated that HAuNFs substrates had very good reproducibility, homogeneous SERS activity, and high SERS effect. The substrates enabled us to successfully obtain the SERS spectra of miR-10a-5p, miR-125a-5p, and miR-196a-5p. The difference spectra among the three kinds of miRNAs were studied to better interpret the spectral differences and identify miRNA expression patterns with high accuracy. The principal component analysis (PCA) of the SERS spectra was used to distinguish among the three kinds of miRNAs. Considering its time efficiency, being labelfree, and its sensitivity, the SERS based on HAuNFs substrates is very promising for miRNA research and plays an important role in early disease detection and prevention.

show abstract

Label-Free Surface-Enhanced Raman Spectroscopy Detection of DNA with Single-Base Sensitivity

Cited by 398 publications

References 47 publications

Current and Emerging Technologies for Rapid Detection of Pathogens

Current and Emerging Technologies for Rapid Detection of Pathogens

Development of graphene oxide-wrapped gold nanorods as robust nanoplatform for ultrafast near-infrared SERS bioimaging

Rapid Detection and Identification of miRNAs by Surface-Enhanced Raman Spectroscopy Using Hollow Au Nanoflowers Substrates

Contact Info

Product

Resources

About