Label-free surface-enhanced Raman spectroscopy for detection of colorectal cancer and precursor lesions using blood plasma

Feng, Shouhua; Wang, Wenbo; Tai, Isabella T.; Chen, Guannan; Chen, Rong; Zeng, Haishan

doi:10.1364/boe.6.003494

Cited by 56 publications

(31 citation statements)

References 38 publications

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“…These results indicated that SERS spectra intensified with progression of the disease. Feng et al 127 explored the use of SERS-based blood plasma analysis for the early detection of precancerous lesions and found that the diagnostic sensitivity and specificity for differentiating polyp samples were 71.4% and 95.6% from CRC and 91.3% and 80% from normal subjects.…”

Section: Bloodmentioning

confidence: 99%

Biomedical optical spectroscopy for the early diagnosis of gastrointestinal neoplasms

2017

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Gastrointestinal cancer is a leading contributor to cancer-related morbidity and mortality worldwide. Early diagnosis currently plays a key role in the prognosis of patients with gastrointestinal cancer. Despite the advances in endoscopy over the last decades, missing lesions, undersampling and incorrect sampling in biopsies, as well as invasion still result in a poor diagnostic rate of early gastrointestinal cancers. Accordingly, there is a pressing need to develop noninvasive methods for the early detection of gastrointestinal cancers. Biomedical optical spectroscopy, including infrared spectroscopy, Raman spectroscopy, diffuse scattering spectroscopy and autofluorescence, is capable of providing structural and chemical information about biological specimens with the advantages of non-destruction, non-invasion and reagent-free and waste-free analysis and has thus been widely investigated for the diagnosis of oesophageal, gastric and colorectal cancers. This review will introduce the advances of biomedical optical spectroscopy techniques, highlight their applications for the early detection of gastrointestinal cancers and discuss their limitations.

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Section: Bloodmentioning

confidence: 99%

Biomedical optical spectroscopy for the early diagnosis of gastrointestinal neoplasms

2017

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“…However, the conventional Raman spectroscopy technique has many disadvantages. Because of typical Raman cross sections are between 10 −30 and 10 −25 cm 2 per molecule, Raman scattering signal is very weak [6]. Moreover, in order to avoid the damage to the cell sample, the applicable maximum intensity of the excitation laser is limited.…”

Section: Introductionmentioning

confidence: 99%

Leukemia cells detection based on electroporation assisted surface-enhanced Raman scattering

Lin

et al. 2017

Biomed. Opt. Express

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Abstract:In this study, an electroporation-based surface-enhanced Raman scattering (SERS) technique was employed to differentiate the human myeloid leukemia cells from the normal human bone marrow mononuclear cells with the aim to develop a fast and label-free method for leukemia cell screening. The Ag nanoparticles were delivered into living cells by electroporation, and then high quality SERS spectra were successfully obtained from 60 acute promyelocytic leukemia cells (HL60 cell line), 60 chronic myelogenous leukemia cells (K562 cell line) and 60 normal human bone marrow mononuclear cells (BMC). Principal component analysis (PCA) combined with linear discriminant analysis (LDA) differentiated the leukemia cell SERS spectra (HL60 plus K562) from normal cell SERS spectra (BMC) with high sensitivity (98.3%) and specificity (98.3%). Furthermore, partial least squares (PLS) approach was employed to develop a diagnostic model. The model successfully predicted the unidentified subjects with a diagnostic accuracy of 96.7%. This exploratory work demonstrates that the electroporation-based SERS technique combined with PCA-LDA and PLS diagnostic algorithms possesses great promise for cancer cell screening.

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“…176 Silver nanoparticles were directly mixed with blood plasma from 76 samples (43 samples came from patients with pathologically confirmed nasopharyngeal carcinomas and 33 from healthy volunteers) and the SERS spectra showed distinct variation between the two patient groups for a variety of bands, while PCA scores were compared to demonstrate the potential for discrimination. In the years that followed, the same group published more studies of nasopharyngeal cancer (NPC) 177 and numerous other SERS studies that took the same exploratory approach towards the detection of different types of cancers, including gastric cancer, [178][179][180] colorectal cancer, 181,182 and cervical cancer. 183 Surface-enhanced Raman spectroscopy spectra obtained from the 2014 NPC study (Figure 12) demonstrated that different tumor (T) stages in NPC have distinct spectral differences.…”

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confidence: 99%

Raman Spectroscopy of Blood and Blood Components

et al. 2017

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Blood is a bodily fluid that is vital for a number of life functions in animals. To a first approximation, blood is a mildly alkaline aqueous fluid (plasma) in which a large number of free-floating red cells (erythrocytes), white cells (leucocytes), and platelets are suspended. The primary function of blood is to transport oxygen from the lungs to all the cells of the body and move carbon dioxide in the return direction after it is produced by the cells' metabolism. Blood also carries nutrients to the cells and brings waste products to the liver and kidneys. Measured levels of oxygen, nutrients, waste, and electrolytes in blood are often used for clinical assessment of human health. Raman spectroscopy is a nondestructive analytical technique that uses the inelastic scattering of light to provide information on chemical composition, and hence has a potential role in this clinical assessment process. Raman spectroscopic probing of blood components and of whole blood has been on-going for more than four decades and has proven useful in applications ranging from the understanding of hemoglobin oxygenation, to the discrimination of cancerous cells from healthy lymphocytes, and the forensic investigation of crime scenes. In this paper, we review the literature in the field, collate the published Raman spectroscopy studies of erythrocytes, leucocytes, platelets, plasma, and whole blood, and attempt to draw general conclusions on the state of the field.

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Label-free surface-enhanced Raman spectroscopy for detection of colorectal cancer and precursor lesions using blood plasma

Cited by 56 publications

References 38 publications

Biomedical optical spectroscopy for the early diagnosis of gastrointestinal neoplasms

Biomedical optical spectroscopy for the early diagnosis of gastrointestinal neoplasms

Leukemia cells detection based on electroporation assisted surface-enhanced Raman scattering

Raman Spectroscopy of Blood and Blood Components

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