Label-free three-dimensional observations and quantitative characterisation of on-chip vasculogenesis using optical diffraction tomography

Lee, Chungha; Kim, Seung Gyu; Hugonnet, Hervé; Park, WeiSun; Jeon, Jessie S.; Park, YongKeun

doi:10.1101/2020.01.01.892620

Cited by 3 publications

(1 citation statement)

References 42 publications

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“…ODTs have been used for imaging various types of biological samples suspended in solution or cultured on 2D surfaces [38][39][40][41][42]. More recently, they have also been applied to samples with extended thicknesses or cells cultured in 3D environments [43][44][45].…”

Section: Introductionmentioning

confidence: 99%

Label-free monitoring of 3D cortical neuronal growth in vitro using optical diffraction tomography

Lee¹,

Yoon

Han

et al. 2021

Preprint

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The highly complex central nervous systems of mammals are often studied using three-dimensional (3D) in vitro primary neuronal cultures. A coupled confocal microscopy and immunofluorescence labeling are widely utilized for visualizing the 3D structures of neurons. However, this requires fixation of the neurons and is not suitable for monitoring an identical sample at multiple time points. Thus, we propose a label-free monitoring method for 3D neuronal growth based on refractive index tomograms obtained by optical diffraction tomography. The 3D morphology of the neurons was clearly visualized, and the developmental processes of neurite outgrowth in 3D spaces were analyzed for individual neurons.

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Section: Introductionmentioning

confidence: 99%

Label-free monitoring of 3D cortical neuronal growth in vitro using optical diffraction tomography

Lee¹,

Yoon

Han

et al. 2021

Preprint

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Fluid–Matrix Interface Triggers a Heterogeneous Activation of Macrophages

Kim

Lee

et al. 2020

ACS Appl. Bio Mater.

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Spontaneous activation of macrophages in response to inflammation is a key part of innate immunity and host defense. Macrophages represent a heterogeneous population of cells with different phenotypic profiles performing distinct functions in host defense. Although a spectrum of macrophage activation stages exists in an inflamed region, the effect of local physical conditions on the heterotypic activation of macrophages is unknown. Here, we introduce an in vivo fluid–matrix interface analogous culture platform, an asymmetric microenvironment, facilitating the formation of macrophage aggregates (MAs). Macrophages were self-assembled to form MAs of ∼100 μm diameter at the collagen matrix–medium interface upon phorbol-12-myristate-13-acetate treatment. The macrophages within the half-embedded MAs into the matrix were heterogeneously activated, resulting in inhomogeneous cell–cell and cell–matrix interactions within the aggregates. Our demonstration may aid in a better understanding of the acquisition of macrophage heterogeneity in response to tissue-specific microenvironments.

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Imaging microphysiological systems: a review

Peel

Jackman

2021

American Journal of Physiology-Cell Physiology

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Microphysiological Systems (MPS), often referred to as 'organ-on-chips' are microfluidic-based in vitro models that aim to recapitulate the dynamic chemical and mechanical microenvironment of living organs. MPS promise to bridge the gap between in vitro and in vivo models, and ultimately improve the translation from pre-clinical animal studies to clinical trials. However, despite the explosion of interest in recent years, and the obvious rewards for such models which could improve R&D efficiency and reduce drug attrition in the clinic, the pharmaceutical industry has been slow to fully adopt this technology. The ability to extract robust, quantitative information from MPS at scale is a key requirement if these models are to impact drug discovery and the subsequent drug development process. Microscopy imaging remains a core technology that enables the capture of information at the single cell level and with subcellular resolution. Furthermore, such imaging techniques can be automated, increasing throughput, enabling compound screening. In this review we discuss a range of imaging techniques that have been applied to MPS of varying focus, such as organoids and organ-chip-type models. We outline the opportunities these technologies can bring in terms of understanding mechanistic biology, but also how they could be used in higher-throughput screens, widening the scope of their impact in drug discovery. We discuss the associated challenges of imaging these complex models and the steps required to enable full exploitation. Finally, we discuss the requirements for MPS, if they are to be applied at a scale necessary to support drug discovery projects.

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Label-free three-dimensional observations and quantitative characterisation of on-chip vasculogenesis using optical diffraction tomography

Cited by 3 publications

References 42 publications

Label-free monitoring of 3D cortical neuronal growth in vitro using optical diffraction tomography

Label-free monitoring of 3D cortical neuronal growth in vitro using optical diffraction tomography

Fluid–Matrix Interface Triggers a Heterogeneous Activation of Macrophages

Imaging microphysiological systems: a review

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