Label-free virtual staining of neutrophil extracellular traps (NETs) in microfluidics

Petchakup, Chayakorn; Wong, Sallene; Dalan, Rinkoo; Hou, Han Wei

doi:10.1039/d3lc00398a

Cited by 2 publications

(1 citation statement)

References 18 publications

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“…Aside from the visualization of the cell-cell interaction, another important advantage of these assays is the ability to study neutrophil behavior and functions in real-time. For example, studies have shown that when using biomimetic microfluidic assays, neutrophil migration ( Boribong et al., 2019 ) and NETosis ( Petchakup et al., 2023 ) can be observed. Furthermore, biomimetic microfluidic assays can be used to observe the process of neutrophils eliminating pathogens such as fungal clusters ( Alex et al., 2020 ) and bacteria ( Ellett et al., 2019 ) in real-time.…”

Section: Emerging In Vitro Models – the Promise Of...mentioning

confidence: 99%

The critical role of neutrophil-endothelial cell interactions in sepsis: new synergistic approaches employing organ-on-chip, omics, immune cell phenotyping and in silico modeling to identify new therapeutics

Liu,

Langston,

Prabhakarpandian

et al. 2024

Front. Cell. Infect. Microbiol.

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Sepsis is a global health concern accounting for more than 1 in 5 deaths worldwide. Sepsis is now defined as life-threatening organ dysfunction caused by a dysregulated host response to infection. Sepsis can develop from bacterial (gram negative or gram positive), fungal or viral (such as COVID) infections. However, therapeutics developed in animal models and traditional in vitro sepsis models have had little success in clinical trials, as these models have failed to fully replicate the underlying pathophysiology and heterogeneity of the disease. The current understanding is that the host response to sepsis is highly diverse among patients, and this heterogeneity impacts immune function and response to infection. Phenotyping immune function and classifying sepsis patients into specific endotypes is needed to develop a personalized treatment approach. Neutrophil-endothelium interactions play a critical role in sepsis progression, and increased neutrophil influx and endothelial barrier disruption have important roles in the early course of organ damage. Understanding the mechanism of neutrophil-endothelium interactions and how immune function impacts this interaction can help us better manage the disease and lead to the discovery of new diagnostic and prognosis tools for effective treatments. In this review, we will discuss the latest research exploring how in silico modeling of a synergistic combination of new organ-on-chip models incorporating human cells/tissue, omics analysis and clinical data from sepsis patients will allow us to identify relevant signaling pathways and characterize specific immune phenotypes in patients. Emerging technologies such as machine learning can then be leveraged to identify druggable therapeutic targets and relate them to immune phenotypes and underlying infectious agents. This synergistic approach can lead to the development of new therapeutics and the identification of FDA approved drugs that can be repurposed for the treatment of sepsis.

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Section: Emerging In Vitro Models – the Promise Of...mentioning

confidence: 99%

The critical role of neutrophil-endothelial cell interactions in sepsis: new synergistic approaches employing organ-on-chip, omics, immune cell phenotyping and in silico modeling to identify new therapeutics

Liu,

Langston,

Prabhakarpandian

et al. 2024

Front. Cell. Infect. Microbiol.

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Imaging and Therapy of Tumors Based on Neutrophil Extracellular Traps

Hao,

Liu,

Wang

et al. 2024

Small Science

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Neutrophil extracellular traps (NETs) formed by neutrophils are netlike scaffolds that mainly contain DNA and a variety of granule proteins. Many stimuli can lead to the NET formation through independent molecular pathways. Clinically, the abundance of NETs is correlated with poor tumor prognosis. The biological actions of NETs are complex and diverse, including promoting tumor progression, awakening the dormant cancer cells, and resulting in immunosuppression in support of tumor growth and metastasis. Therefore, NET‐associated pathological processes provide an important clue for both diagnostic imaging and alternative therapies for many kinds of cancers. In recent years, scientists’ efforts have focused on developing novel imaging probes to visualize NETs and therapeutic strategies by degrading NETs or inhibiting its formation to block their pro‐tumoral functions. In this review, the development and evaluation of NETs‐targeted imaging and intervention progress for tumor therapy are focused on.

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Label-free virtual staining of neutrophil extracellular traps (NETs) in microfluidics

Abstract: Neutrophils are the most abundant circulating white blood cells and one of their critical functions to eliminate pathogenic threats includes the release of extracellular DNA, also known as neutrophil extracellular...

Cited by 2 publications

References 18 publications

The critical role of neutrophil-endothelial cell interactions in sepsis: new synergistic approaches employing organ-on-chip, omics, immune cell phenotyping and in silico modeling to identify new therapeutics

The critical role of neutrophil-endothelial cell interactions in sepsis: new synergistic approaches employing organ-on-chip, omics, immune cell phenotyping and in silico modeling to identify new therapeutics

Imaging and Therapy of Tumors Based on Neutrophil Extracellular Traps

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