Label‐free Voltammetric Immunosensor for Prostate Specific Antigen Detection

Abstract

This work describes a sensitive voltammetric immunosensor for label‐free electroanalysis of the prostate specific antigen (PSA), the main biomarker of prostate cancer. A gold electrode was firstly modified with the optimum self‐assembled monolayer (SAM), 1,6‐hexanedithiol, followed by the subsequent adsorption of gold nanoparticles (AuNPs) and, then, the monoclonal antibody to recognize PSA was immobilized. The influence of the most significant experimental variables (SAM type and incubation time, AuNPs deposition, antibody concentration and bovine serum albumin immobilization) on the biosensor response was studied by microscopy and voltammetry techniques. The electroanalytical detection was based on the interaction between PSA antibody and PSA via square‐wave voltammetry using ferrocyanide/ferricyanide as electrochemical redox indicator. Using the proposed immunosensor, PSA was specifically detected within the linear range between 0.2 and 200 ng mL−1 with 0.01 ng mL−1 as limit of detection. The immunosensor allows accurate, reproducible and sensitive (22.7 % reduction mL ng−1) detection in a concentration range useful for clinical purposes.

3D-printed electrode as a new platform for electrochemical immunosensors for virus detection

“…5 A shows the representative CV obtained to the Np detection with concentration ranging from 30 to 580 μg mL −1 . The CV data showed a dependence from the Np concentration added and the electrochemical response of the probe, indicating an inert kinetic barrier for the electron-transfer as the Np concentration rises, that is likely related to the antigen-antibody immunocomplex formation, as it acts as an insulating on the device surface [ [35] , [36] , [37] ].

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Futuristic Silicon Photonic Biosensor with Nanomaterial Enhancement for PSA Detection

“…Table 1 shows the proposed method with already reported PSA immunosensors. The LOD values reported by Gupta et al [52], Oliveira et al [57], and Jang et al [58] are 3.6 x 10 -1 , 5.9 x 10 -1 ng/mL and 1.18 ng/mL, respectively. These reported values are respectively 2.1, 3.5 and 6.9 orders of magnitude higher than the LOD obtained in this current work.…”

Futuristic Silicon Photonic Biosensor with Nanomaterial Enhancement for PSA Detection

“…Table 1 shows the proposed method with already reported PSA immunosensors. The LOD values reported by Gupta et al [52], Oliveira et al [57], and Jang et al [58] are 3.6 × 10 −1 , 5.9 × 10 −1 ng/mL and 1.18 ng/mL, respectively. These reported values are, respectively, 2.1, 3.5, and 6.9 orders of magnitude higher than the LOD obtained in this current work.…”