Labelfree mapping and profiling of altered lipid homeostasis in the rat hippocampus after traumatic stress: Role of oxidative homeostasis

Kelley, D. Parker; Chaichi, Ardalan; Duplooy, Alexander; Singh, D. P.; Gartia, Manas Ranjan; Francis, Joseph

doi:10.1016/j.ynstr.2022.100476

Cited by 6 publications

(2 citation statements)

References 76 publications

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“…We further analyzed the Raman images of the tissues by the direct classical least squares (DCLS) method [ 62 ] to profile lipid changes on the surface of tissues before and after treatment. The DCLS method is proper when we have a reference spectrum of the component we are profiling: lipids spectra.…”

Section: Resultsmentioning

confidence: 99%

Multimodal Imaging of Pancreatic Cancer Microenvironment in Response to an Antiglycolytic Drug

Sheikh,

Agrawal,

Roy

et al. 2023

Adv Healthcare Materials

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Lipid metabolism and glycolysis play crucial roles in the progression and metastasis of cancer, and the use of 3‐bromopyruvate (3‐BP) as an antiglycolytic agent has shown promise in killing pancreatic cancer cells. However, developing an effective strategy to avoid chemoresistance requires the ability to probe the interaction of cancer drugs with complex tumor‐associated microenvironments (TAMs). Unfortunately, no robust and multiplexed molecular imaging technology is currently available to analyze TAMs. In this study, we demonstrate the simultaneous profiling of three protein biomarkers using SERS nanotags and antibody‐functionalized nanoparticles in a syngeneic mouse model of pancreatic cancer. This allows for comprehensive information about biomarkers and TAM alterations before and after treatment. Our multimodal imaging techniques include surface‐enhanced Raman spectroscopy (SERS), immunohistochemistry, polarized light microscopy, second harmonic generation (SHG) microscopy, fluorescence lifetime imaging microscopy (FLIM), and untargeted liquid chromatography and mass spectrometry (LC‐MS) analysis. The study reveals the efficacy of 3‐BP in treating pancreatic cancer and identifies drug treatment‐induced lipid species remodeling and associated pathways through bioinformatics analysis.This article is protected by copyright. All rights reserved

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Section: Resultsmentioning

confidence: 99%

Multimodal Imaging of Pancreatic Cancer Microenvironment in Response to an Antiglycolytic Drug

Sheikh,

Agrawal,

Roy

et al. 2023

Adv Healthcare Materials

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“…Where increase total cholesterol, triglycerides, low-density lipoprotein (LDL) levels, and decrease high-density lipoprotein (HDL) levels in serum. [3][4][5]. Profiling lipids in samples might advance the discovery of diagnostic, prognostic, and monitoring disease biomarkers.…”

Section: Introductionmentioning

confidence: 99%

Biochemical Study For Evaluation of some Clinical Parameters as Predictors of Myocardial Infarction in Patients With Type 2 Diabetes Mellitus Undergoing Elective Percutaneous Coronary Intervention

Rasha Hasan Jasim,

Sara Sattar Jabbar

2023

J. K. Chem. Sci.

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When blood flow to the heart muscle is impeded, fat and cholesterol build up on the inner wall of the heart, causing coronary heart disease (CHD), myocardial infarction (MI), and sudden cardiac death are just a few of the diseases that can occur in CAD as a result of atherosclerosis. A catheter is used during Percutaneous Coronary Intervention (PCI), also known as angioplasty, a non-surgical procedure to squeeze a balloon or stent into a narrowing area to restore blood flow to the myocardium. Therefore, it is treated with medication to widen or open up the veins in the heart muscle that contain plaque accumulation that results in stenosis in order to avoid the coronary artery blockage from occurring again. The key CVD risk factor is lipid profile and some biochemicals parameters, and each kind these parameters are associated with an increase in the incidence of MI. Anabolic processes, energy metabolism, and blood sugar levels are all controlled by the hormone insulin. Glycosylated hemoglobin (HbA1c) is frequently used to measure the average blood glucose levels of a person based on the age. The resulting values also help to diagnose diabetes mellitus by determining the blood sugar level. Elevated aminotransferase levels indicating liver function, have attracted great concern as potential novel markers of cardiovascular risk assessment. Although blood urea nitrogen (BUN), creatinine (Cr), and electrolytes is not the mainstay of diagnosis in MI patients, they may have a role in providing a more detailed view of the complications and mortality rates.

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Mapping lipid species remodeling in high fat diet-fed mice: Unveiling adipose tissue dysfunction with Raman microspectroscopy

Sheikh,

Liu,

Burk

et al. 2024

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biolog

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Labelfree mapping and profiling of altered lipid homeostasis in the rat hippocampus after traumatic stress: Role of oxidative homeostasis

Cited by 6 publications

References 76 publications

Multimodal Imaging of Pancreatic Cancer Microenvironment in Response to an Antiglycolytic Drug

Multimodal Imaging of Pancreatic Cancer Microenvironment in Response to an Antiglycolytic Drug

Biochemical Study For Evaluation of some Clinical Parameters as Predictors of Myocardial Infarction in Patients With Type 2 Diabetes Mellitus Undergoing Elective Percutaneous Coronary Intervention

Mapping lipid species remodeling in high fat diet-fed mice: Unveiling adipose tissue dysfunction with Raman microspectroscopy

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