Labeling and Imaging Mesenchymal Stem Cells with Quantum Dots

Collins, Maria C.; Cascio, Wayne E.; Kypson, Alan P.; Muller‐Borer, Barbara J.

doi:10.1007/978-1-61779-953-2_15

Cited by 17 publications

(12 citation statements)

References 9 publications

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“…In contrast, MSCs can also be directly labelled with nanoparticles such as GNPs. Other examples include superparamagnetic iron oxide nanoparticles and quantum dots, in which the former is detectable by MRI [38], while the latter is a fluorescence-based technique [39]. …”

Section: Discussionmentioning

confidence: 99%

Micro-Computed Tomography Detection of Gold Nanoparticle-Labelled Mesenchymal Stem Cells in the Rat Subretinal Layer

Mok

Leow

Koh

et al. 2017

IJMS

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Mesenchymal stem cells are widely used in many pre-clinical and clinical settings. Despite advances in molecular technology; the migration and homing activities of these cells in in vivo systems are not well understood. Labelling mesenchymal stem cells with gold nanoparticles has no cytotoxic effect and may offer suitable indications for stem cell tracking. Here, we report a simple protocol to label mesenchymal stem cells using 80 nm gold nanoparticles. Once the cells and particles were incubated together for 24 h, the labelled products were injected into the rat subretinal layer. Micro-computed tomography was then conducted on the 15th and 30th day post-injection to track the movement of these cells, as visualized by an area of hyperdensity from the coronal section images of the rat head. In addition, we confirmed the cellular uptake of the gold nanoparticles by the mesenchymal stem cells using transmission electron microscopy. As opposed to other methods, the current protocol provides a simple, less labour-intensive and more efficient labelling mechanism for real-time cell tracking. Finally, we discuss the potential manipulations of gold nanoparticles in stem cells for cell replacement and cancer therapy in ocular disorders or diseases.

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Section: Discussionmentioning

confidence: 99%

Micro-Computed Tomography Detection of Gold Nanoparticle-Labelled Mesenchymal Stem Cells in the Rat Subretinal Layer

Mok

Leow

Koh

et al. 2017

IJMS

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“…These comprise atoms for releasing electrons and cadmium as one of the chemicals. Most of their usage is limited to tracking stem cells undergoing differentiation and migration [75, 76]. These are photostable and have longer longevity.…”

Section: Influence Of the Biophysical Microenvironment On Stem Cell Rmentioning

confidence: 99%

Nanostructured scaffold as a determinant of stem cell fate

et al. 2016

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The functionality of stem cells is tightly regulated by cues from the niche, comprising both intrinsic and extrinsic cell signals. Besides chemical and growth factors, biophysical signals are important components of extrinsic signals that dictate the stem cell properties. The materials used in the fabrication of scaffolds provide the chemical cues whereas the shape of the scaffolds provides the biophysical cues. The effect of the chemical composition of the scaffolds on stem cell fate is well researched. Biophysical signals such as nanotopography, mechanical forces, stiffness of the matrix, and roughness of the biomaterial influence the fate of stem cells. However, not much is known about their role in signaling crosstalk, stem cell maintenance, and directed differentiation. Among the various techniques for scaffold design, nanotechnology has special significance. The role of nanoscale topography in scaffold design for the regulation of stem cell behavior has gained importance in regenerative medicine. Nanotechnology allows manipulation of highly advanced surfaces/scaffolds for optimal regulation of cellular behavior. Techniques such as electrospinning, soft lithography, microfluidics, carbon nanotubes, and nanostructured hydrogel are described in this review, along with their potential usage in regenerative medicine. We have also provided a brief insight into the potential signaling crosstalk that is triggered by nanomaterials that dictate a specific outcome of stem cells. This concise review compiles recent developments in nanoscale architecture and its importance in directing stem cell differentiation for prospective therapeutic applications.

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“…Another product frequently used for stem cell labelling is quantum dots (QDs; Wang, Xu, & Ow, ). QDs are fluorescent nanoparticles, which are resistant to metabolic degradation (Collins, Gunst, Cascio, Kypson, & Muller‐Borer, ), have minimal cytotoxic effects, and have been shown not to interfere with proliferation of rat bone marrow MSCs in culture (Muller‐Borer, Collins, Gunst, Cascio, & Kypson, ). Visualization of QDs in labelled rat MSCs is possible in cardiomyocyte cocultures for up to 7 days (Muller‐Borer et al, ), and QDs have been demonstrated to be present in canine hearts 8 weeks following delivery of QD‐labelled human MSCs (Rosen, Kelly, Schuldt, et al, ).…”

Section: Introductionmentioning

confidence: 99%

Persistence of fluorescent nanoparticle‐labeled bone marrow mesenchymal stem cells in vitro and after intra‐articular injection

Grady

Britton

Hinrichs

et al. 2018

J Tissue Eng Regen Med

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Mesenchymal stem cells (MSCs) improve the osteoarthritis condition, but the fate of MSCs after intra-articular injection is unclear. We used fluorescent nanoparticles (quantum dots [QDs]) to track equine MSCs (QD-labelled MSCs [QD-MSCs]) in vivo after intra-articular injection into normal and osteoarthritic joints. One week after injection of QD-MSCs, unlabelled MSCs, or vehicle, we determined the presence of QD-MSCs in synovium and articular cartilage histologically. In vitro, we evaluated the persistence of QDs in MSCs and whether QDs affected proliferation, immunophenotype, or differentiation. In joints injected with QD-MSCs, labelled cells were identified on the synovial membrane and significantly less often on articular cartilage, without differences between normal and osteoarthritic joints. Joints injected with QD-MSCs and MSCs had increased synovial total nucleated cell count and protein compared with vehicle-injected joints. In vitro, QDs persisted in nonproliferating cells for up to 8 weeks (length of the study), but QD fluorescence was essentially absent from proliferating cells within two passages (approximately 3 to 5 days). QD labelling did not affect MSC differentiation into chondrocytes, adipocytes, and osteocytes. QD-MSCs had slightly different immunophenotype from control cells, but whether this was due to an effect of the QDs or to drift during culture is unknown.QD-MSCs can be visualized in histological sections 1 week after intra-articular injection and are more frequently found in the synovial membrane versus cartilage in both normal and osteoarthritic joints. QDs do not alter MSC viability and differentiation potential in vitro. However, QDs are not optimal markers for long-term tracking of MSCs, especially under proliferative conditions.

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Labeling and Imaging Mesenchymal Stem Cells with Quantum Dots

Cited by 17 publications

References 9 publications

Micro-Computed Tomography Detection of Gold Nanoparticle-Labelled Mesenchymal Stem Cells in the Rat Subretinal Layer

Micro-Computed Tomography Detection of Gold Nanoparticle-Labelled Mesenchymal Stem Cells in the Rat Subretinal Layer

Nanostructured scaffold as a determinant of stem cell fate

Persistence of fluorescent nanoparticle‐labeled bone marrow mesenchymal stem cells in vitro and after intra‐articular injection

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