Labeling of fatty acids with 4-bromomethyl-7-methoxycoumarin via crown ether catalyst for fluorimetric detection in high-performance liquid chromatography

1-Pyrenyldiazomethane as a fluorescent labeling reagent for liquid chromatographic determination of carboxylic acids

“…Although the ICP-AES or MS techniques are capable of providing sub-part-per-million detection limits, these detection limits are necessarily degraded when the amount of available sample is less than approximately 1 mL (1). Part-per-million (ppm) detection limits are achievable with the electron probe technique at sample volumes in the 100-pL range, and these detection limits are not reduced as the sample volume increases (2). Thus, the need exists for a trace element analysis technique having sub-ppm detection limits for sample volumes in the 100 pL to 100 nL range.…”

“…Many kinds of precolumn fluorescent derivatization reagents (1-14) have been developed for the determination of carboxylic acids such as fatty acids and prostaglandins by high-performance liquid chromatography (HPLC). Halogenomethyl (1)(2)(3)(4)(5) or halogenoacyl (6,7) aromatic compounds such as 4-bromomethyl-7-methoxycoumarin (1, 2) and 1bromoacetylpyrene (6) have extensively been examined. The reactions of these reagents with carboxylic acids, however, proceeded only at elevated temperature in the presence of base catalysts.…”

Estimation of Glycerides and Free Fatty Acid in Oils Extracted From Various Seeds from the Indian Region by NMR Spectroscopy

“…Several studies were carried out on the development and improvement of analytical methods for biodiesel production and determining the fuel quality. Analytical procedures reported in the literature include chromatographic methods [4][5][6] (GC, HPLC, GPC) and spectroscopic methods [7][8][9][10][11][12] ( 1 H, 13 C NMR and FTIR), which are extensively used for monitoring the production, synthesis and quality of biodiesel. High-resolution NMR ( 1 H, 13 C NMR) spectroscopy offers many advantages over existing analytical methods, because it allows the rapid, simultaneous, noninvasive and nondestructive study in the multi-component system of fat, oil and biodiesel.…”

Analysis of long-chain fatty acids in beer by an HPLC-fluorescence detection method

“…Fatty acids are difficult to monitor directly at low concentrations by liquid chromatography; however, derivatization of the acids with moieties that enhance either ultraviolet absorption or fluorescence can greatly facilitate detectability (Nimura and Kinoshita, 1980;Lloyd, 1980;Lam and Grushka, 1978;Tsuchiya, 1982;Dünges, 1978). 9-Anthrylmethyl esters that formed from carboxylic acids with 9-anthradiazomethane show both high absorptivity and intense fluorescence and are readily separated on reversed-phase LC columns (Greeley, 1974).…”