“…To our knowledge, no general method has been reported previously that is single-step and useful for all protecting groups, such as acetyl, benzoyl, and phenoxyacetyl, in which the protecting group have been installed on the base moiety of cytidine and 2'-deoxycytidine without prior O-protection and in the absence of a catalyst. Additionally, present methodology may be extended to a variety of selective N-acylation of cytidine using other carboxylic acids such as t-butylphenoxyacetyl (t-BPA), 21 isopropyl-phenoxyacetyl (IPAC), and isopropoxyacetyl (IPOAC) 5 to achieve milder and faster deprotection in the oligonucleotides synthesis.…”