Labisia pumila protects the bone of estrogen-deficient rat model: A histomorphometric study

Fathilah, Siti Noor; Shuid, Ahmad Nazrun; Mohamed, Norazlina; Muhammad, Norliza; Soelaiman, Ima Nirwana

doi:10.1016/j.jep.2012.04.029

Cited by 25 publications

(31 citation statements)

References 40 publications

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“…alata (LPva), a herb used for women’s health, was found to produce beneficial effects similar to estrogen on bone biomarkers in postmenopausal osteoporosis animal model [15]. These bone protective effects were further confirmed by bone histomorphometric analysis [14-16] and bone biomechanical strength [15-17]. …”

Section: Introductionmentioning

confidence: 99%

Labisia pumila regulates bone-related genes expressions in postmenopausal osteoporosis model

Fathilah

Mohamed

Muhammad

et al. 2013

BMC Complement Altern Med

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BackgroundLabisia Pumila var. alata (LPva) has shown potential as an alternative to estrogen replacement therapy (ERT) in prevention of estrogen-deficient osteoporosis. In earlier studies using postmenopausal model, LPva was able to reverse the ovariectomy-induced changes in biochemical markers, bone calcium, bone histomorphometric parameters and biomechanical strength. The mechanism behind these protective effects is unclear but LPva may have regulated factors that regulate bone remodeling. The aim of this study is to determine the bone-protective mechanism of LPva by measuring the expressions of several factors involved in bone formative and resorptive activities namely Osteoprotegerin (OPG), Receptor Activator of Nuclear Factor kappa-B Ligand (RANKL), Macrophage-Colony Stimulating Factor (MCSF) and Bone Morphogenetic Protein-2 (BMP-2).MethodsThirty-two female Wistar rats were randomly divided into four groups: Sham-operated (Sham), ovariectomized control (OVXC), ovariectomized with Labisia pumila var. alata (LPva) and ovariectomized with ERT (Premarin®) (ERT). The LPva and ERT were administered via daily oral gavages at doses of 17.5 mg/kg and 64.5 μg/kg, respectively. Following two months of treatment, the rats were euthanized and the gene expressions of BMP-2, OPG, RANKL and MCSF in the femoral bones were measured using a branch - DNA technique.ResultsThe RANKL gene expression was increased while the OPG and BMP-2 gene expressions were reduced in the OVXC group compared to the SHAM group. There were no significant changes in the MCSF gene expressions among the groups. Treatment with either LPva or ERT was able to prevent these ovariectomy-induced changes in the gene expressions in ovariectomized rats with similar efficacy.ConclusionLPva may protect bone against estrogen deficiency-induced changes by regulating the RANKL, OPG and BMP-2 gene expressions.

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Section: Introductionmentioning

confidence: 99%

Labisia pumila regulates bone-related genes expressions in postmenopausal osteoporosis model

Fathilah

Mohamed

Muhammad

et al. 2013

BMC Complement Altern Med

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“…Other parameters were not significantly different compared to sham even though declining values were observed. However, in another study it was observed that cellular parameters deteriorated in the ovariectomized group as compared to the sham group [24]. The discrepancies seen in the histomorphometry parameters between ovariectomized and sham-operated groups were surprising and it may be attributed to the small sample size used in our study.…”

Section: Discussionmentioning

confidence: 53%

The effects of Cosmos caudatus (ulam raja) on dynamic and cellular bone histomorphometry in ovariectomized rats

Mohamed

Sahhugi²,

Ramli

et al. 2013

BMC Res Notes

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BackgroundCosmos caudatus is a local plant which has antioxidant properties and contains high calcium. It is also reported to be able to strengthen the bone. This report is an extension to previously published article in Evidence Based Complementary and Alternative Medicine (doi:10.1155/2012/817814). In this study, we determined the effectiveness of C. caudatus as an alternative treatment for osteoporosis due to post-menopause by looking at the dynamic and cellular paramaters of bone histomorphometry.MethodsForty female Wistar rats were divided into four groups i.e. sham operated, ovariectomized, ovariectomized treated with calcium 1% ad libitum and ovariectomized force-fed with 500 mg/kg C. caudatus extract. Treatment was given six days a week for eight weeks.ResultsDynamic and cellular histomorphometry parameters were measured. C. caudatus increased double-labeled surface (dLS/BS), mineral appositional rate (MAR), osteoid volume (OV/BV) and osteoblast surface (Ob.S/BS). C. caudatus also gave better results compared to calcium 1% in the osteoid volume (OV/BV) parameter.ConclusionsC. caudatus at the 500 mg/kg dose may be an alternative treatment in restoring bone damage that may occur in post-menopausal women.

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“…A previous study by Nazrun et al ., (2011) has found that LP was able to increase the bone formation marker and reduce the bone resorption marker [23]. Other studies reported that there were improvements in histomorphometric parameters of ovariectomized rats supplemented with LP [39]. This has led to our interest in studying the antioxidative effect of LP on bone.…”

Section: Discussionmentioning

confidence: 99%

Time and Dose-Dependent Effects of Labisia pumila on Bone Oxidative Status of Postmenopausal Osteoporosis Rat Model

Effendy

Shuid

2014

Nutrients

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Postmenopausal osteoporosis can be associated with oxidative stress and deterioration of antioxidant enzymes. It is mainly treated with estrogen replacement therapy (ERT). Although effective, ERT may cause adverse effects such as breast cancer and pulmonary embolism. Labisia pumila var. alata (LP), a herb used traditionally for women’s health was found to protect against estrogen-deficient osteoporosis. An extensive study was conducted in a postmenopausal osteoporosis rat model using several LP doses and duration of treatments to determine if anti-oxidative mechanisms were involved in its bone protective effects. Ninety-six female Sprague-Dawley rats were randomly divided into six groups; baseline group (BL), sham-operated (Sham), ovariectomised control (OVXC), ovariectomised (OVX) and given 64.5 μg/kg of Premarin (ERT), ovariectomised and given 20 mg/kg of LP (LP20) and ovariectomised and given 100 mg/kg of LP (LP100). The groups were further subdivided to receive their respective treatments via daily oral gavages for three, six or nine weeks of treatment periods. Following euthanization, the femora were dissected out for bone oxidative measurements which include superoxide dismutase (SOD), glutathione peroxidase (GPx) and malondialdehyde (MDA) levels. Results: The SOD levels of the sham-operated and all the treatment groups were significantly higher than the OVX groups at all treatment periods. The GPx level of ERT and LP100 groups at the 9th week of treatment were significantly higher than the baseline and OVX groups. MDA level of the OVX group was significantly higher than all the other groups at weeks 6 and 9. The LP20 and LP100 groups at the 9th week of treatment had significantly lower MDA levels than the ERT group. There were no significant differences between LP20 and LP100 for all parameters. Thus, LP supplementations at both doses, which showed the best results at 9 weeks, may reduce oxidative stress which in turn may prevent bone loss via its anti-oxidative property.

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Labisia pumila protects the bone of estrogen-deficient rat model: A histomorphometric study

Cited by 25 publications

References 40 publications

Labisia pumila regulates bone-related genes expressions in postmenopausal osteoporosis model

Labisia pumila regulates bone-related genes expressions in postmenopausal osteoporosis model

The effects of Cosmos caudatus (ulam raja) on dynamic and cellular bone histomorphometry in ovariectomized rats

Time and Dose-Dependent Effects of Labisia pumila on Bone Oxidative Status of Postmenopausal Osteoporosis Rat Model

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