Lable-free quadruple signal amplification strategy for sensitive electrochemical p53 gene biosensing

Wang, Zonghua; Xia, Jianfei; Song, Daimin; Zhang, Feifei; Yang, Min; Gui, Rijun; Xia, Lin; Bi, Sai; Xia, Yanzhi

doi:10.1016/j.bios.2015.09.011

Cited by 30 publications

(15 citation statements)

References 41 publications

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“…Copyright 2006 Wiley. (B) Initiated by hybridization of the target p53 gene to the signaling probes on an AuNP, the NEase-catalyzed cyclic cleave of the signaling probes produces multiple copies of peroxidase-mimicking DNAzymes . Reproduced from Biosens.…”

Section: Amplified Detection Of Nucleic Acids and Proteins Using Pero...mentioning

confidence: 99%

“…77 , Wang, Z. H.; Xia, J. F.; Song, D. M.; Zhang, F. F.; Yang, M.; Gui, R. J.; Xia, L.; Bi, S.; Xia, Y. Z. Lable-free quadruple signal amplification strategy for sensitive electrochemical p53 gene biosensing, pp 157–163 (ref ) Copyright 2016 with permission from Elsevier. (C) Stepwise removal of the ssDNA template from the duplex DNA by Exo-III releases the blocked peroxidase-mimicking DNAzyme .…”

Section: Amplified Detection Of Nucleic Acids and Proteins Using Pero...mentioning

confidence: 99%

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DNAzyme-Mediated Assays for Amplified Detection of Nucleic Acids and Proteins

et al. 2017

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Section: Amplified Detection Of Nucleic Acids and Proteins Using Pero...mentioning

confidence: 99%

Section: Amplified Detection Of Nucleic Acids and Proteins Using Pero...mentioning

confidence: 99%

DNAzyme-Mediated Assays for Amplified Detection of Nucleic Acids and Proteins

et al. 2017

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“…3(b)] and dramatically enhanced the electrochemical signals. 23,[31][32][33][34] In many cases, AuNPs were used to immobilize hemin/Gq on the solid surface of a GCE. Nanocomposites, such as porous platinum nanotubes, 29 platinum-palladium nanowires, 30 and palladium nanoparticles decorated with graphene-molybdenum disulfide, 23 were also used as scaffolds to increase the number of hemin/Gq complexes, facilitating co-immobilization of other components, such as enzymes and electron mediators.…”

Section: Scaffold-based Increasementioning

confidence: 99%

“…This strategy has been demonstrated for the successful detection of miRNA (8 aM), 42 a short DNA model (10 fM), 9 and a partial DNA sequence of the p53 gene (0.30 fM). 34 The target-triggered EXPAR has also been employed to generate inhibition removal strands that allow the autonomous formation of Gq sequences by hybridizing to the loop part of a stem-loop probe; this strategy has been used to detect miRNA 36 and an avian influenza A (H7N9) viral DNA model. 24 The detection limits were 5.36 fM and 9.4 fM, respectively.…”

Section: Inhibitor Removal Strategymentioning

confidence: 99%

Hemin/G-quadruplex Complex as a Signal Generator for Electrochemical Assays of Bioanalytes

Funabashi

2016

Electrochemistry

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Single-stranded deoxyribonucleic acid (ssDNA) with guanine-rich sequences can form four-stranded structures so called G-quadruplex (Gq), which binds hemin, an iron-containing porphyrin. The hemin/Gq is an electroactive complex and exhibits peroxidase activity, and thus the hemin/Gq complexes have attracted great attention as a signal generator. This short review surveys the use of the hemin/Gq complex as an electrochemical signal generator. Electrochemical methods for detecting the hemin/Gq complex, approaches to signal amplification, and targeting strategies are herein discussed. Compared with protein-based signal generators, hemin/Gq complexes have some advantages: a wide variety of ssDNA sequences with various chemical modifications are easy to obtain, they can be immobilized on an electrode easily, and they can be designed as desired to connect functional domains. Also there are many molecular-biological tools to handle them. Hemin/Gq complexes have been successfully used to detect bioanalytes ranging from low molecular weight compound to macromolecules such as proteins, specific nucleic acids, or living cancer cells. Such bioanalytes are critical to the investigation of cellular function. Thus, DNAbased probes that contain hemin/Gq as a signal generator are a promising tool for the electrochemical analysis of cellular function, offering a competent alternative to a conventional protein-based signal generator.

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“…Due to their wide solubility and introduction of a surface charge, ionic liquid-functionalized graphene (IL-graphene) sheets with good dispersibility and long-term stability in various solvents have been synthesized by the Niu group [23]. In addition, IL-graphene based electrochemical sensors and biosensors have also been extensively reported in literature for direct electron transfer of various redox enzymes and the detection of various types of compounds, such as p53 gene [24], bovine serum albumin [25], bovine hemoglobin [26], and rutin [27].…”

Section: Introductionmentioning

confidence: 99%

Enhanced voltammetric determination of dopamine using a glassy carbon electrode modified with ionic liquid-functionalized graphene and carbon dots

Zhuang

Wang

et al. 2016

Microchim Acta

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The authors describe a dopamine (DA) sensor based on a glassy carbon electrode modified with a composite film composed of carbon dots (C-dots) and graphene functionalized with an ionic liquid. The C-dots were functionalized with carboxy groups whose negative charge promotes electrostatic attraction to the protonated amino groups in DA. The presence of an imidazole cation in the IL facilitates interaction with the C-dots and DA via electrostatic interactions and π-stacking forces. Under optimal conditions, the modified GCE display improved electrochemical response to DA compared to a bare GCE, or a GCE modified with C-dots or IL-graphene only. The oxidation current, measured best at a potential of 0.22 V (vs. Ag/AgCl) is linearly related to the DA concentration in the 0.1 to 600 μM range, with a 30 nM detection limit at a signal-to-noise ratio of 3. Ascorbic acid does not interfere even in large excess, and the sensor is stable for at least a month. The modified GCE was applied to the determination of DA in spiked fetal bovine serum and gave satisfactory results.

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Lable-free quadruple signal amplification strategy for sensitive electrochemical p53 gene biosensing

Cited by 30 publications

References 41 publications

DNAzyme-Mediated Assays for Amplified Detection of Nucleic Acids and Proteins

DNAzyme-Mediated Assays for Amplified Detection of Nucleic Acids and Proteins

Hemin/G-quadruplex Complex as a Signal Generator for Electrochemical Assays of Bioanalytes

Enhanced voltammetric determination of dopamine using a glassy carbon electrode modified with ionic liquid-functionalized graphene and carbon dots

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