Objective. To study the effect of minimum nurse staffing requirements on the subsequent employment of nursing home support staff. Data Sources. Nursing home data from the Online Survey Certification and Reporting (OSCAR) System merged with state nurse staffing requirements. Study Design. Facility-level housekeeping, food service, and activities staff levels are regressed on nurse staffing requirements and other controls using fixed effect panel regression. Data Extraction Method. OSCAR surveys from 1999 to 2004. Principal Findings. Increases in state direct care and licensed nurse staffing requirements are associated with decreases in the staffing levels of all types of support staff. Conclusions. Increased nursing home nurse staffing requirements lead to input substitution in the form of reduced support staffing levels. Key Words. Nursing homes, regulation, staffing requirements, support staff, housekeeping, food service, activities staff Nurse staffing is an important input in the production of high-quality nursing home (NH) care. Responding to concerns about inadequate nurse staffing levels, between 1999 and 2004, 14 states enacted or strengthened minimum nurse staffing policies. Without nurse staffing requirements, NH administrators choose the optimal amount of nurse staff and non-nurse inputs that maximize the facility's objectives. While requirements may guarantee a minimum level of nurse staff availability, administrators face resource constraints and increases in requirements may cause reductions in the use of non-nurse inputs. This substitution of inputs is likely to result in reduced employment of support staff, such as housekeepers, food service, and activities workers. This article studies whether NHs engage in input substitution by using fewer support staff after changes in nurse staffing requirements.