Laboratory-Acquired Severe Acute Respiratory Syndrome

Lim, Poh Lian; Kurup, Asok; Gopalakrishna, Gowri; Chan, Kwai Peng; Wong, Christopher W.; Ng, Lee Ching; Se-Thoe, Su Yun; Oon, Lynette Lin Ean; Bai, Xinlai; Stanton, Lawrence W.; Ruan, Yijun; Miller, Lance D.; Vega, Vinsensius B.; James, Lyn; Ooi, Peng Lim; Kai, Chew Suok; Olsen, Sonja J.; Ang, Brenda; Leo, Yee Sin

doi:10.1056/nejmoa032565

Cited by 149 publications

(104 citation statements)

References 10 publications

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“…According to the World Health Organization, up to 2004 Apr 21, SARS coronavirus infected more than 8000 people in various countries worldwide and caused approximately 800 deaths [4] . Although SARS infection of human beings has been contained through infection-control measures, resurgence is still a threat because the causative agent remaining in animal reservoirs is not fully understood, and sporadic cases continue to be reported in Singapore [5,6] , Taiwan [7] and mainland China [8,9] . There are no specific vaccines and effective drugs currently available for SARS-CoV [1,2,10,11] .…”

Section: Introductionmentioning

confidence: 99%

Preparation and development of equine hyperimmune globulin F(ab')2 against severe acute respiratory syndrome coronavirus1

Wang

et al. 2005

Acta Pharmacologica Sinica

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Aim: The resurgence of severe acute respiratory syndrome (SARS) is still a threat because the causative agent remaining in animal reservoirs is not fully understood, and sporadic cases continue to be reported. Developing high titers of anti-SARS hyperimmune globulin to provide an alternative pathway for emergent future prevention and treatment of SARS. Methods: SARS coronavirus (CoV)F69 (AY313906) and Z2-Y3 (AY394989) were isolated and identified from 2 different Cantonese onset SARS patients. Immunogen was prepared from SARS-CoV F69 strain. Six health horses were immunized 4 times and serum was collected periodically to measure the profile of specific IgG and neutralizing antibodies using indirect enzyme-linked immunosorbent assay and a microneutralization test. Sera were collected in large amounts at the peak, where IgG was precipitated using ammonium sulphate and subsequently digested with pepsin. The product was then purified using anion-exchange chromatography to obtain F(ab') 2 fragments. Results: The specific IgG and neutralizing antibody titers peaked at approximately week 7 after the first immunization, with a maximum value of 1:14210. The sera collected at the peak were then purified. Fragment of approximately 15 g F(ab') 2 was obtained from 1 litre antiserum and the purity was above 90% with the titer of 1:5120, which could neutralize the other strain (SARS-CoV Z2-Y3) as well. Conclusion: This research provides a viable strategy for the prevention and treatment of SARS coronavirus infection with equine hyperimmune globulin, with the purpose of combating any resurgence of SARS.

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Section: Introductionmentioning

confidence: 99%

Preparation and development of equine hyperimmune globulin F(ab')2 against severe acute respiratory syndrome coronavirus1

Wang

et al. 2005

Acta Pharmacologica Sinica

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“…Three of these incidents were attributed to breaches in laboratory biosafety, and resulted in one or more cases of SARS (Singapore [11][12][13], Taipei [14] and Beijing [15,16]). Fortunately, only one of these incidents resulted in secondary transmission outside of the laboratory.…”

Section: Sarsmentioning

confidence: 99%

Postgraduate Course ERS Munich 2006 - Avian influenza and SARS: updates on the big stories

2006

Breathe

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“…The incidents of laboratory-acquired SARS in Singapore [54], Taiwan [55] and China [56] have strongly indicated that despite biosafety guidelines laid down by WHO [49] and CDC [57], breaches were possible. In August 2003, a 27-year old graduate student acquired SARS infection in Singapore, due to accidental transmission while working with the West Nile virus in a high containment laboratory [54].…”

Section: Biocontainment and Researchmentioning

confidence: 99%

“…In August 2003, a 27-year old graduate student acquired SARS infection in Singapore, due to accidental transmission while working with the West Nile virus in a high containment laboratory [54]. It was believed that the West Nile virus was somehow contaminated with the SARS-CoV that was propagated in the same laboratory.…”

Section: Biocontainment and Researchmentioning

confidence: 99%

Strategies adopted and lessons learnt during the severe acute respiratory syndrome crisis in Singapore

Dmeri¹,

Sgh²

2004

Reviews in Medical Virology

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In Singapore, the military was actively involved in the containment of the outbreak of severe acute respiratory syndrome (SARS) last year. The outbreak started in February 2003 with three Singapore travellers to Hong Kong. At that time, nothing was known about the aetiological agent of the atypical pneumonia that was termed SARS. Unfortunately one of the travellers was a super-spreader, defined as a person with high efficiency for virus transmission, and was responsible for the expansion of the national outbreak. Not only was the Singapore military involved in contact tracing of personnel and enforcement of home quarantine, military-affiliated research institutes were also involved in providing diagnostic support. This review reconstructs the events that took place during the SARS outbreak, focusing on the special support arising from complementing the military-affiliated laboratory with the public health laboratory. A description of the diagnostic findings is provided in chronological order. The review ends with lessons Singapore learnt from the SARS crisis, stressing the importance of national preparedness for future outbreaks.

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Laboratory-Acquired Severe Acute Respiratory Syndrome

Cited by 149 publications

References 10 publications

Preparation and development of equine hyperimmune globulin F(ab')2 against severe acute respiratory syndrome coronavirus1

Preparation and development of equine hyperimmune globulin F(ab')2 against severe acute respiratory syndrome coronavirus1

Postgraduate Course ERS Munich 2006 - Avian influenza and SARS: updates on the big stories

Strategies adopted and lessons learnt during the severe acute respiratory syndrome crisis in Singapore

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