Laboratory animal allergy: the measurement of airborne urinary allergens and the effects of different environmental conditions

Edwards, R. G.; Beeson, Malcolm F.; Dewdney, Janet M.

doi:10.1258/002367783781070696

Cited by 47 publications

(19 citation statements)

References 6 publications

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“…An important difference with other studies (20,22,26) is that our study was performed at different sites, which should account for sufficient variation in the various variables. Despite the large number of sites in our study, we could not find any association between ambient air allergen concentrations and the rate of ventilation and relative humidity in the room, a finding which is in contrast to those of other studies (24,25,36). The reasons for these discrepancies are not clear, but they could be associated with the limited influence of ventilation when compared with the influence of other strong determinants of exposure or the dependence between the ventilation rate and other determinants of exposure.…”

Section: Discussioncontrasting

confidence: 99%

Determinants of airborne rat and mouse urinary allergen exposure

Hollander¹,

Heederik²,

Doekes³

et al. 1998

Scand J Work Environ Health

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Section: Discussioncontrasting

confidence: 99%

Determinants of airborne rat and mouse urinary allergen exposure

Hollander¹,

Heederik²,

Doekes³

et al. 1998

Scand J Work Environ Health

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“…High RUA concentrations occurred when the animals were housed on wood based, contact litter (geometric mean (GM) sawdust pg/m'; woodchip [6][7][8][9][10][11][12][13][14][15][16] pg/m'). The use of noncontact absorbent pads was associated with a significant decrease in RUA concentrations (GM 2-47 pg/m'; p < 0-0001).…”

mentioning

confidence: 99%

Reduction of airborne allergenic urinary proteins from laboratory rats.

Gordon

Tee

Lowson

et al. 1992

Occupational and Environmental Medicine

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Allergy and asthma caused by proteins of laboratory animals, particularly rats and mice, are the most important occupational health hazards for the scientists and technicians who work with such animals. The influence of different cage litters, cage design, and stock density on measured rat urinary aeroallergen (RUA) concentrations has been examined in a room housing male rats, to determine practical means to reduce allergen concentration in animal laboratories. Eight hour static air samples were taken at 2 I/mi and the RUA concentrations measured by radioallergosorbent test (RAST) inhibition. High RUA concentrations occurred when the animals were housed on wood based, contact litter (geometric mean (GM) sawdust 7-79 pg/m'; woodchip 6-16 pg/m'). The use of noncontact absorbent pads was associated with a significant decrease in RUA concentrations (GM 2-47 pg/m'; p < 0-0001). Rat urinary aeroallergen concentrations fell more than fourfold when the animals were housed on woodbased, contact litter in filter top cages rather than conventional open top cages (GM filter top 0 33 pg/m'; open top 1-43 pg/in; p < 0-0001). The number ofrats (stock density) strongly influenced the RUA concentration and a linear relation was found between the log, allergen concentration and stock density under these study conditions. The measurement of airborne particle size on cleaning out days showed that all litter types generated similar sized particles: more than 80% of the RUA was carried on particles larger than 8 pm in diameter for all litter types. The findings

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“…As mentioned above, the method described is not based on using IgE antibodies responsible for the im mediate type of reactions as in radioallergosorbent test inhibition [12,14,17]. Therefore, the method does not necessarily measure the determinants responsible for atopic allergy, because anti-BEA antiserum is likely to react also with non-allergenic determinants of BEA.…”

Section: Discussionmentioning

confidence: 99%

Enzyme-Linked Immunosorbent Assay (ELISA) Inhibition Method to Estimate the Level of Airborne Bovine Epidermal Antigen in Cowsheds

Virtanen¹,

Louhelainen

Mäntyjärvi³

1986

Int Arch Allergy Immunol

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A method based on inhibition of the enzyme-linked immunosorbent assay was developed for measuring airborne bovine epidermal antigen (BEA) in cowsheds. BEA extract served as the solid-phase reactant competing with the sample for binding of a rabbit immune serum. A sample of swine epithelial extract was used to ascertain the specificity of the reaction. Cowshed air samples were collected according to a standardized volumetric filter method. A specific inhibiting activity was detected in all samples tested corresponding to concentrations between 137ng/m³ and 19.8 μg/m³. The intertest coefficient of variation of cowshed samples was 9.5%.

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Laboratory animal allergy: the measurement of airborne urinary allergens and the effects of different environmental conditions

Cited by 47 publications

References 6 publications

Determinants of airborne rat and mouse urinary allergen exposure

Determinants of airborne rat and mouse urinary allergen exposure

Reduction of airborne allergenic urinary proteins from laboratory rats.

Enzyme-Linked Immunosorbent Assay (ELISA) Inhibition Method to Estimate the Level of Airborne Bovine Epidermal Antigen in Cowsheds

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