Laboratory animal models for esophageal cancer

Nair, Dhanya Venugopalan; Reddy, A Gopala

doi:10.14202/vetworld.2016.1229-1232

Cited by 12 publications

(5 citation statements)

References 41 publications

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“…Previously reported metastatic mouse models have been mostly subcutaneous tumor models . Furthermore, there are a few studies, which have reported orthotopic metastatic mouse models of esophageal cancer, however, of our knowledge, other orthotopic metastatic mouse models of cocultured EC with CAFs have not been reported . We have established and reported this esophageal orthotopic xenograft model, in which tumor volume and metastatic activity were evaluated by luciferase emission .…”

Section: Discussionmentioning

confidence: 99%

Cancer‐associated fibroblasts (CAFs) promote the lymph node metastasis of esophageal squamous cell carcinoma

Kashima

Noma

Ohara

et al. 2018

Intl Journal of Cancer

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Lymph node metastasis is a pathognomonic feature of spreading tumors, and overcoming metastasis is a challenge in attaining more favorable clinical outcomes. Esophageal cancer is an aggressive tumor for which lymph node metastasis is a strong poor prognostic factor, and the tumor microenvironment (TME), and cancer-associated fibroblasts (CAFs) in particular, has been implicated in esophageal cancer progression. CAFs play a central role in the TME and have been reported to provide suitable conditions for the progression of esophageal cancer, similar to their role in other malignancies. However, little is known concerning the relevance of CAFs to the lymph node metastasis of esophageal cancer. Here, we used clinical samples of esophageal cancer to reveal that CAFs promote lymph node metastasis and subsequently verified the intercellular relationships in vitro and in vivo using an orthotopic metastatic mouse model. In the analysis of clinical samples, FAP + CAFs were strongly associated with lymph node metastasis rather than with other prognostic factors. Furthermore, CAFs affected the ability of esophageal cancer cells to acquire metastatic phenotypes in vitro; this finding was confirmed by data from an in vivo orthotopic metastatic mouse model showing that the number of lymph node metastases increased upon injection of cocultured cancer cells and CAFs. In summary, we verified in vitro and in vivo that the accumulation of CAFs enhances the lymph node metastasis of ESCC. Our data suggest that CAF targeted therapy can reduce lymph node metastasis and improve the prognosis of patients with esophageal cancer in the future.

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Section: Discussionmentioning

confidence: 99%

Cancer‐associated fibroblasts (CAFs) promote the lymph node metastasis of esophageal squamous cell carcinoma

Kashima

Noma

Ohara

et al. 2018

Intl Journal of Cancer

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“…Briefly, IR treatment was performed with an 8 Gy dose at 2 Gy/min on the tumor position in dorsolateral flanks with other body areas of mice protected with a lead cover. On 28, 35, 42 days post-inoculation, the tumor sizes were measured with a caliper [10]. Tumor volume (mm 3 ) was calculated as L 9 l 2 9 0.5 (L is the longest diameter, and l is the shortest diameter) [11].…”

Section: Human Esc Xenograft Mouse Model and Target Ir Treatmentmentioning

confidence: 99%

Direct Downregulation of B-Cell Translocation Gene 3 by microRNA-93 Is Required for Desensitizing Esophageal Cancer to Radiotherapy

et al. 2017

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“…14,15 ALA treatment has shown to be effective in many POI animal models, such as in cyclophosphamide-induced and 4-vinyl cyclohexene dioxide induced POI rats. 16,17 Although the activities of ALA in the prevention of POI have been discovered, in-depth studies of mechanisms are lacking. In addition, a comprehensive elucidation of ALA's biological pathways and biomarkers has not yet been completed.…”

Section: Introductionmentioning

confidence: 99%

Bioinformatics and integrated pharmacology network to identify the therapeutic targets and potential molecular mechanism of alpha‐lipoic acid on primary ovarian insufficiency

Kong,

Cho,

Hwang

et al. 2023

J of Cellular Biochemistry

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Women experiencing primary ovarian insufficiency (POI) are more likely to experience infertility, and its incidence is increasing worldwide annually. Recently, the role of alpha‐lipoic acid (ALA) in the treatment of POI has been reported. However, details of the potential pharmacological targets and related molecular pathways of ALA remain unclear and need to be elucidated. Thus, this study aims to elucidate the potential therapeutic target and related molecular mechanism of ALA on POI. First, the potential targets of POI and ALA‐related targets were downloaded from online public databases. Subsequently, the overlapped target genes between POI and ALA were acquired, and gene ontology (GO), Kyoto encyclopedia of genes and genomes (KEGG) analysis, protein–protein interaction (PPI) networks were performed and constructed. Finally, molecular docking was performed to verify protein‐to‐protein effect. A total of 152 potential therapeutic targets were identified. The biological processes of the intersecting targets were mainly involved in the cellular response to peptides, response to xenobiotic stimuli, and response to peptide hormones. The highly enriched pathways were the cAMP, PI3K/AKT, estrogen, progesterone mediated oocyte maturation, and apoptosis signaling pathways. The top 10 hub targets for ALA in the treatment of POI were STAT3, STAT1, CASP3, MTOR, PTGS2, CASP8, HSP90AA1, PIK3CA, MAPK1, and ESR1. The binding between ALA and all top hub targets were verified using the molecular docking analysis. In summary, using the systematic integrated pharmacology network and bioinformatics analysis, this study illustrated that ALA participates in the treatment of POI via multiple targets and multiple pathways mechanisms.

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Laboratory animal models for esophageal cancer

Cited by 12 publications

References 41 publications

Cancer‐associated fibroblasts (CAFs) promote the lymph node metastasis of esophageal squamous cell carcinoma

Cancer‐associated fibroblasts (CAFs) promote the lymph node metastasis of esophageal squamous cell carcinoma

Direct Downregulation of B-Cell Translocation Gene 3 by microRNA-93 Is Required for Desensitizing Esophageal Cancer to Radiotherapy

Bioinformatics and integrated pharmacology network to identify the therapeutic targets and potential molecular mechanism of alpha‐lipoic acid on primary ovarian insufficiency

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