Laboratory – Based Bioremediation of Hydrocarbon Polluted Mangrove Swamp Soil in the Niger Delta Using Poultry Wastes

Ezekoye, Charles Chibueze; Amakoromo, Ebiokpo Rebecca; Ibiene, A. A.

doi:10.9734/mrji/2017/15153

Cited by 7 publications

(8 citation statements)

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“…The factorial experiment (4 x 3) was laid out in a completely randomized design with three replicates for each SEO contamination level whereby thirty six (36) polyethylene bags were set up. As reported by Ezekoye, Amakoromo, and Ibiene (2017), out of the thirty six (36) polyethylene bags, nine (9) polyethylene bags were kept as control while one hundred and fifty grammes (150 g) of each organic amendment; compost, CPH and cow dung was added and expansively mixed with the soil contaminated with 5, 10 and 15% SEO. Excluding the nine (9) polyethylene bags used as control, all the remaining twenty seven (27) polyethylene bags were comprehensively mixed with one hundred and fifty milliliters (150 mL) of the bacterial co-culture.…”

Section: Bioremediation Experimentsmentioning

confidence: 99%

Effect of bacterial co-culture and organic amendments on the bioremediation of hydrocarbons in a soil contaminated with spent engine oil

Adeleye¹,

Yerima

Nkereuwem³

et al. 2022

Acta Sci. Biol. Sci.

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Bioenhancement of hydrocarbonoclastic microorganisms with suitable nutrients has a huge impact in achieving positive bioremediation of polluted environments. This study was conducted to assess the bio-enhancing effect of some organic amendments on Streptococcus pyogenes and Enterococcus faecalis co-culture with a view to remediating spent engine oil (SEO) contaminated soil. Top soil (1.5 kg) was autoclaved and thereafter contaminated with SEO at three levels. The contaminated soil was inoculated with bacterial co-culture (150 mL) and subsequently bioenhanced with compost, processed cocoa pod husk (CPH) and cow dung. The factorial experiment was laid out in completely randomized design. Concentrations of total petroleum hydrocarbon (TPH) and selected polycyclic aromatic hydrocarbons (PAH) were estimated on the first day, 5th week and 10th week of incubation. Results obtained show that bacterial co-culture bioenhanced with compost produced the most significant TPH reductions (1318 and 261 mg kg-1) on 10% SEO contaminated soil at the 5th and 10th week respectively (p<0.05). Again, bacterial co-culture bioenhanced with compost produced the most significant PAH reductions (65.9 and 55.8 mg kg-1) on 10% SEO contaminated soil at the 5th and 10th week respectively (p<0.05). The significant bioremediation capabilities exhibited by the bacterial co-culture bioenhanced with organic amendments in this study has made these bioremediation agents potential candidates in remediating soils impacted with petroleum hydrocarbons.

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Section: Bioremediation Experimentsmentioning

confidence: 99%

Effect of bacterial co-culture and organic amendments on the bioremediation of hydrocarbons in a soil contaminated with spent engine oil

Adeleye¹,

Yerima

Nkereuwem³

et al. 2022

Acta Sci. Biol. Sci.

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“…This powdered material was sieved into fine form, and then sterilized by autoclaving at 121°C for 15 min. according to Ezekoye et al, (2017). On the other hand, 10 kg of the collected CD was sun-dried and crushed into powder using pestle and mortar.…”

Section: Collection and Processing Of The Organic Amendmentsmentioning

confidence: 99%

“…It was equally sieved into fine form, autoclaved at 121°C for 15 min. as described by Ezekoye et al, (2017). They were subsequently stored in a container and labeled as powdered CPH and CD, respectively.…”

Section: Collection and Processing Of The Organic Amendmentsmentioning

confidence: 99%

“…The composting process lasted for 20 days as described by Vishan et al, (2107). The generated compost was subsequently autoclaved at 121°C for 15 min., to exclude the extraneous influence of the microbes as recommended by Ezekoye et al, (2017).…”

Section: Collection and Processing Of The Organic Amendmentsmentioning

confidence: 99%

“…In this study, 36 polyethylene bags were used for the heavy metals' decontamination assay conducted according to Nkereuwem et al, (2010); Ezekoye et al, (2017). Apart from the 9 experimental bags that were used as controls, about 150 g of each sterilized biostimulant (compost, powdered CPH, and powdered CD) was added individually and mixed thoroughly with each set of the soils contaminated with the SEO (at 5%, 10% and 15% levels).…”

Section: Heavy Metals' Decontamination Assaymentioning

confidence: 99%

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Effect of organic amendments on the decontamination potential of heavy metals by Staphylococcus aureus and Bacillus cereus in soil contaminated with spent engine oil

2019

Novel Research in Microbiology Journal

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This study was conducted to assess the decontamination of heavy metals of an environmental concern in spent engine oil (SEO) contaminated soil, through the adoption of Staphylococcus aureus and Bacillus cereus cocultures, isolated from Dutse mechanic village, Nigeria. About 1.5 kg of autoclaved soil was contaminated with SEO at three levels. The sterilized soil was then amended with compost, powdered cocoa pod husk (CPH), and powdered cow dung (CD). Subsequently, bio-augmentation with bacterial co-cultures (150 ml) was done. Heavy metal concentrations; Arsenic (As), Cadmium (Cd), Chromium (Cr), Nickel (Ni) and Lead (Pb) were estimated at the commencement, at the fifth and tenth week of the study. The factorial experiment was laid out in a completely randomized design (CRD). Results indicate that bio-stimulation adopted through the amendments did not have significant effects on the bacterial decontamination of soil especially at the 5 th week (P> 0.05). CD only influenced the decontamination of Cd at 5% contamination level (0.0008 mg\ kg), compared with compost that recorded the least effect (0.00360 mg\ kg) at the 5 th week. Meanwhile, CPH had the highest decontamination effect on Cr (0.004 and 0.000 mg\ kg) at 10% and 15% contamination levels; respectively, at the 5 th week. However at the 10 th week, complete removal of As was influenced by compost, CPH, and CD using 5%, 10% and 15% SEO contamination levels, respectively (P< 0.05). The decontamination efficacies of S. aureus and B. cereus recorded in this study is an indication of their potentialities for application in bioremediation of heavy metals.

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Fungal diversity associated with crude oil-impacted soil undergoing in-situ bioremediation

Ezekoye

Chikere

Okpokwasili

2018

Sustainable Chemistry and Pharmacy

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Laboratory – Based Bioremediation of Hydrocarbon Polluted Mangrove Swamp Soil in the Niger Delta Using Poultry Wastes

Cited by 7 publications

References 0 publications

Effect of bacterial co-culture and organic amendments on the bioremediation of hydrocarbons in a soil contaminated with spent engine oil

Effect of bacterial co-culture and organic amendments on the bioremediation of hydrocarbons in a soil contaminated with spent engine oil

Effect of organic amendments on the decontamination potential of heavy metals by Staphylococcus aureus and Bacillus cereus in soil contaminated with spent engine oil

Fungal diversity associated with crude oil-impacted soil undergoing in-situ bioremediation

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