Laboratory‐Based Investigation of Denture Sonication Method in Patients with <i>Candida</i>‐Associated Denture Stomatitis

Perić, Mirjana; Radunović, Milena; Pekmezović, Marina; Marinković, Jelena; Živković, Rade; Arsenijevic, Valentina Arsić

doi:10.1111/jopr.12610

Cited by 11 publications

(6 citation statements)

References 35 publications

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“…Serbia has a large older population; almost 20% are older than 65 years (1,352,948 population size). In this group about 15% of the population wear dentures (202,942 cases), and our laboratory based investigation showed a prevalence of Candida stomatitis of 32.8% [ 25 ], which makes for 66,991 OC cases in denture wearers. Also, severe Candida -related prosthetic stomatitis (Newton classification—severity type III) was found in 18.3% cases and was mainly associated with mixed Candida spp.…”

Section: Resultsmentioning

confidence: 99%

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Estimated Burden of Serious Fungal Diseases in Serbia

Arsenijevic

Denning

2018

JoF

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For the first time, we aimed to estimate the burden of serious fungal infections or diseases (SFD) and highlight national epidemiological features in Serbia. Data on population and underlining conditions were extracted from the Statistical Office of the Republic of Serbia, World Bank, the Institute of Public Health of Serbia, the World Health Organization, National reference laboratory for medical mycology, the national registries of Serbian professional societies, and relevant publications. The population structure/inhabitants in 2016 (not including the autonomous region Kosovo & Metohija) was 7,058,322; with 6,041,743 adults (85.6%). The populations at risk (total cases per year) were: HIV infected 2441; acute myeloid leukemia 212; stem cell transplantation 151; solid organ transplants 59; chronic obstructive pulmonary disease 250,302; adult asthmatics 311,806; adult cystic fibrosis 65; pulmonary tuberculosis 898; lung cancer 7260; intensive care unit admissions 19,821; and renal support 520. Annual fungal disease cases estimated are: candidemia 518; invasive aspergillosis 619; Candida peritonitis 187; Pneumocystis jirovecii pneumonia 62; cryptococcosis 5; mucormycosis or fusariosis 23; severe asthma with fungal sensitization 10,393; allergic bronchopulmonary aspergillosis 9094; chronic pulmonary aspergillosis 448, recurrent Candida vaginitis 135,303; oral candidiasis 208,489; esophageal candidiasis 173, fungal keratitis 70; tinea capitis 300; and onychomycosis 342,721. We expect that 156,825 people suffer from serious SFD each year (2221/100,000), and 409 dies annually. Additionally, the prevalence of superficial infections exceeds 1,008,995 cases (14,295/100,000). The first Rhinosporidium outbreak in Europe was associated with Serbian Silver Lake. The plant pathogen Fusarium seems to be emerging in Serbian pediatric haematooncology settings. Candida auris and endemic mycoses have not been observed to date. These general estimates provide a primer for further efforts to study fungal epidemiology in Serbia.

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Section: Resultsmentioning

confidence: 99%

“…Also, severe Candida -related prosthetic stomatitis (Newton classification—severity type III) was found in 18.3% cases and was mainly associated with mixed Candida spp. or NAC strains which can be difficult to treat [ 25 ]. The incidence data for OC following antibiotic or corticosteroid usage is difficult to estimate.…”

Section: Resultsmentioning

confidence: 99%

Estimated Burden of Serious Fungal Diseases in Serbia

Arsenijevic

Denning

2018

JoF

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“…The choice for the microorganisms to be tested was based in the main goal of the membrane application, that is, the control of oral microbiota: S. mutans (present in the dental biofilm and main microorganism of caries lesion) (25)(26) and C. albicans (main microorganism of oral mucosa infections in adult wearers of prostheses) (27).…”

Section: Discussionmentioning

confidence: 99%

In Vitro Antimicrobial Effect of Bioadhesive Oral Membrane with Chlorhexidine Gel

et al. 2018

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This study aimed to evaluate in vitro the antimicrobial effect of a bioadhesive chitosan-based oral membrane with chlorhexidine for local treatment of infections in the oral tissues. Five oral membranes of different compositions were tested: 5% chitosan (G1); 5% chitosan ± 0.2% chlorhexidine (G2), 5% chitosan ± 0.6% chlorhexidine (G3), 5% chitosan ± 1.0% chlorhexidine (G4), and 5% chitosan ± 2.0% chlorhexidine (G5). Also, five gel types were tested according to the following compositions: 5% chitosan gel (G6), 0.2% chlorhexidine gel (G7), 2.0% chlorhexidine gel (G8), 5% chitosan gel ± 0.2% chlorhexidine gel (G9), and 5% chitosan gel ± 2.0% chlorhexidine gel (G10). The antimicrobial action of the samples was tested against Candida albicans and Streptococcus mutans through antibiogram by measuring the inhibition halos. Data were statistically analyzed by Kruskal-Wallis and one-way ANOVA followed by Tukey test (p<0.05). The 2.0% chlorhexidine membrane (G5) and the disks containing 2.0% chlorhexidine gel (G8) showed the greatest inhibition halos for both microorganisms, with statistically significant difference when compared to others tested groups (p=0.008) only for Candida albicans inhibitions results. All the other formulations of membranes and gels showed inhibition halos, but without statistically significant difference. The bioadhesive chitosan-based oral membrane with 2% chlorhexidine and 2% chlorhexidine gel were the most effective in inhibiting the tested microorganisms.

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“…For cases with denture stomatitis, yeast quantification of mixed Candida spp., and non-albicans Candida using ultrasonication sampling produced better results. This was not possible when using conventional methods, such as swab or oral rinse sampling procedures [16]. The aim of this comparative study was fulfilled.…”

Section: Discussionmentioning

confidence: 99%

“…This is easier and less subjective than surface swabs or denture material retrieval, sectioning, and then culturing [15]. Studies, which focused on the denture stomatitis cases, found that sampling from sonication fluids is accurate, sensitive, and increases the possibility of diagnosing patients with candida-associated lesions [16].…”

Section: Introductionmentioning

confidence: 99%

Comparison of Various Ultra-Sonication Periods’ Protocols on Dentures' Plaque- A Microbiological Study

2017

DSRT

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Aim:The aim of this study is to describe and compare different ultra-sonication protocols used for microbial and candidal sampling, and then test their diagnostic accuracy when correlating microbial loads with a visual plaque score.Methodology: Dentures of consenting subjects were stained and baseline plaque-scores recorded for the fitting and polished surfaces separately. Upper and lower Dentures were sonicated separately in 200 ml distilled-sterile water using one of the following methods (n=10 each): G1: a continuous 30-minutes sonication (Cnt 15 min) cycle, G2: 6 intermittent cycles, (Int 5 min)cycles.G3: a (Cnt 10 min) cycle then 4 (Int 5 min) cycles, G4: a continuous (Cnt 15 min) cycle then 3 (Int 5 min) cycles. G5: a continuous (Cnt 10 min) cycle, then a soaking step (Sk 10 min) then 2 (Int 5 min) cycles. Microbial samples were collected regularly -every 5 minutes-and cultured on two media (Candida selective and non-selective media). Results: G1:The microbial load on non-selective agar began to rise till the third sampling point, then gradually declined with continuous ultrasonications (p>0.05). G2:The microbial load was significantly high in the first then second (Int 5 min) cycles (56.00 ± SE 21.21 @ 5 min, 22.40 ± SE 7.43 @ Int 10 min) (p<0.05). Then it gradually declined in intermittent cycles (p>0.05). G3:The first cycle produced highest numbers (67.40 ± SE 29.02 @ Cnt 10 min) (p<0.05) then gradually decreased in intermittent cycles (p>0.05) till zero. G4:The microbial load was high at the first 15minute cycle (90.20 ± SE 26.72 @ Cnt 15 min) (p<0.05), then sharply decreased in intermittent cycles (p>0.05) till zero. G5:Was similar to G3 till minute 20. The soaking step was contaminated microbiologically, although no ultrasonication was applied (23.8 ± SE 17.03 @ Sk 10 min). Only the continuous 10-minutes ultrasonication protocols (in Gs 1, 2 and 5) had their microbial and candidal counts, on both media, correlate significantly with baseline plaque-scores of the denture as a whole and with each surface separately (Wilcoxon's Signed Ranks Test, p<0.05).

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Laboratory‐Based Investigation of Denture Sonication Method in Patients with Candida‐Associated Denture Stomatitis

Cited by 11 publications

References 35 publications

Estimated Burden of Serious Fungal Diseases in Serbia

Estimated Burden of Serious Fungal Diseases in Serbia

In Vitro Antimicrobial Effect of Bioadhesive Oral Membrane with Chlorhexidine Gel

Comparison of Various Ultra-Sonication Periods’ Protocols on Dentures' Plaque- A Microbiological Study

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