Laboratory Bioassay of Selected Entomopathogenic Nematodes as Mortality Factors of Oulema melanopus (Coleoptera: Chrysomelidae)

Mazurkiewicz, A.; Jakubowska, Magdalena; Tumialis, Dorota; Skrzecz, Iwona; Roik, Kamila; Pezowicz, E.; Gross, Alicja

doi:10.18474/jes18-118

Cited by 6 publications

(4 citation statements)

References 17 publications

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“…The temperature range during the rearing of insects in the climate chamber was 20 ± 1°C. Relative humidity was maintained at 60 ± 5%, and photoperiodic setting was 18L:6D, corresponding to the insect vitality optimum ( Mazurkiewicz et al, 2019 ).…”

Section: Methodsmentioning

confidence: 99%

Physiological Responses Manifested by Some Conventional Stress Parameters and Biophoton Emission in Winter Wheat as a Consequence of Cereal Leaf Beetle Infestation

et al. 2022

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Oulema melanopus L. (Coleoptera: Chrysomelidae) is one of the most serious pests of winter wheat that causes peeling of the epidermis and tissue loss. The complex mapping of the physiological responses triggered by O. melanopus as a biotic stressor in winter wheat has not been fully explored with the help of non-invasive imaging and analytical assays, yet. The aim of the present work was to study the effect of O. melanopus on the physiological processes of winter wheat, especially on the extent of lipid peroxidation and antioxidant activity derived from tissue destruction, as well as photosynthetic ability. The results of the measurements enabled the identification of the antioxidant and lipid-oxidation–related physiological reactions, and they were reflected in the dynamics of non-invasive biophoton emissions. Our non-invasive approach pointed out that in the case of O. melanopus infestation the damage is manifested in tissue loss and the systemic signaling of the biotic stress may have reached other plant parts as well, which was confirmed by the results of antioxidant capacity measurements. These results indicate that the plant reacts to the biotic stress at a whole organizational level. We identified that the antioxidant and lipid-oxidation–related physiological reactions were reflected in the dynamics of two aspects of biophoton emission: delayed fluorescence and ultra-weak bioluminescence. Our research further supported that the non-invasive approach to stress assessment may complete and detail the traditional stress indicators.

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Section: Methodsmentioning

confidence: 99%

Physiological Responses Manifested by Some Conventional Stress Parameters and Biophoton Emission in Winter Wheat as a Consequence of Cereal Leaf Beetle Infestation

et al. 2022

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“…There are two species of cereal leaf beetles in Poland: Oulema melanopus (L.) and Oulema gallaeciana Heyden both belonging to the Chrysomelidae family. The first is more common in the central and northern parts of the country, while the second dominates in south-eastern Poland [2]. O. melanopus is also the most important wheat pest in many other countries [3,4], ranging from central Siberia through Sweden, Great Britain, Spain, western Africa [5,6] to the United States and Canada [5,7].…”

Section: Introductionmentioning

confidence: 99%

“…Our field studies were preceded by laboratory tests to assess the sensitivity of beetles and larvae of O. melanopus to native isolates of entomopathogenic nematodes represented by two species: S. feltiae (four isolates) and Heterorhabditis megidis (Poinar, Jackson and Klein, 1987) (one isolate). The isolate of S. feltiae ZAG 15 was the most effective, causing 100% mortality in both larvae and adult insects [2], and was selected for field studies.…”

Section: Introductionmentioning

confidence: 99%

Foliar Application of Entomopathogenic Nematodes against Cereal Leaf Beetle Oulema melanopus L. (Coleoptera: Chrysomelidae) on Wheat

et al. 2021

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Cereal monocultures are very susceptible to many pests, especially to those living on leaves, which largely affects yield by decreasing its quality. The most dangerous of them is the cereal leaf beetle (Oulema melanopus L.). In cases of heavy infestation by its larvae, the surface of plants may be reduced by 50%, and sometimes even by 80%, with a main yield loss of 10–25%. The aim of the presented study was to assess the efficiency of a native isolate of Steinernema feltiae (Filipjev, 1934) and commercial preparation Larvanem (Heterorhabditis bacteriophora (Poinar, 1975)) in controlling the larvae of O. melanopus, and to reduce crops damage in the field. Nematodes were applied in a dosage of 2 million IJs/m2 as a suspension of 11 litres per square metre. A hand sprinkler with field lance and flat-stream nozzles was used for applications at the lowest working pressure of 3000 hPa. The effectiveness of both nematode species was moderate: 47.8% for S. feltiae isolate and 49.5% for H. bacteriophora. The biggest reduction in leaf damage was found in crops treated with the commercial preparation, where the index of leaf infection was 32%, being more than twofold smaller than that for the control.

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“…Adults were collected using an entomological sweeping net. The temperature range during the rearing of insects in the climate chamber was 20 ± 1 • C. Relative humidity was maintained at 60 ± 5%, and photoperiodic setting was 18L:6D, corresponding to the insect vitality optimum (Mazurkiewicz et al, 2019).…”

Section: Sampling and Experimental Settingmentioning

confidence: 99%

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Laboratory Bioassay of Selected Entomopathogenic Nematodes as Mortality Factors of Oulema melanopus (Coleoptera: Chrysomelidae)

Cited by 6 publications

References 17 publications

Physiological Responses Manifested by Some Conventional Stress Parameters and Biophoton Emission in Winter Wheat as a Consequence of Cereal Leaf Beetle Infestation

Physiological Responses Manifested by Some Conventional Stress Parameters and Biophoton Emission in Winter Wheat as a Consequence of Cereal Leaf Beetle Infestation

Foliar Application of Entomopathogenic Nematodes against Cereal Leaf Beetle Oulema melanopus L. (Coleoptera: Chrysomelidae) on Wheat

Data_Sheet_1.docx

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