Laboratory Colonization of Anopheles aquasalis (Diptera: Culicidae) in Belém, Pará, Brazil

Silva, Ana N. M. da; Santos, Carla Christiani Bastos dos; Lacerda, Raimundo N. L.; Rosa, Edvaldo P. Santa; Souza, Raimundo Tadeu Lessa de; Galiza, Deocleciano; Sucupira, Izis Mônica Carvalho; Conn, Jan E.; Póvoa, Marinete Marins

doi:10.1093/jmedent/43.1.107

Cited by 14 publications

(8 citation statements)

References 10 publications

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“…(25) A colony of Ny. aquasalis was successfully established by the use of forced mating, (26) a technique that forces copulation after the male head has been removed. (27) However, this technique failed when it was applied to Ny.…”

Section: Discussionmentioning

confidence: 99%

Description of an automatic copulation induction system used to establish a free-mating laboratory colony of Nyssorhynchus deaneorum from Brazil

Araújo

Santos

Andrade

et al. 2020

Mem. Inst. Oswaldo Cruz

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BACKGROUND Nyssorhynchus deaneorum is a potential malaria vector because it has been shown to be competent to transmit Plasmodium vivax and Plasmodium falciparum, and because it exhibits antropophilic and endophilic behaviors in some regions of the Amazon. This profile makes Ny. deaneorum a useful mosquito for experiments that model Plasmodium-vector interactions in the Amazon. OBJECTIVE Herein we describe how a free-mating colony of Ny. deaneorum has been established using an automated light stimulation system. METHODS Mosquitoes were captured in São Francisco do Guaporé, Rondônia. The F1 generation was reared until adult emergence at which point copulation was induced using an automatic copulation induction system (ACIS). FINDINGS After four generations, natural mating and oviposition began to occur without light stimulation. The number of pupae and adult mosquitoes increased from the F 5 to F 10 generations. The new Ny. deaneorum colony exhibited susceptibility to P. vivax. MAIN CONCLUSIONS Automated light stimulation is an effective method for establishing an Ny. deaneorum colony under laboratory conditions as it produces enough adults to create a stenogamic colony. The establishment of a stable, P. vivaxsusceptible colony of Ny. deaneorum makes it possible to model parasite-vector interactions and to test novel drug therapies that target parasite development in mosquitoes.

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Section: Discussionmentioning

confidence: 99%

Description of an automatic copulation induction system used to establish a free-mating laboratory colony of Nyssorhynchus deaneorum from Brazil

Araújo

Santos

Andrade

et al. 2020

Mem. Inst. Oswaldo Cruz

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“…Adult Anopheles aquasalis females were raised under standard insectary conditions, 28 ºC with 80% relative humidity and photoperiod of 12 h dark to 12 h light. Adults were fed 10% glucose solution ad libitum, as described by Silva et al (2006). Then, individual midguts were dissected and fixed for 3 hours in 2.5% glutaraldehyde solution in 0.1 M sodium cacodylate buffer pH 7.2 and processed as described below.…”

Section: Methodsmentioning

confidence: 99%

Morphological Aspect of the Midgut of Anopheles aquasalis (Curry, 1932) Insecta: Diptera

Ferreira

Lima²,

Rocha

et al. 2008

Int. J. Morphol.

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SUMMARY:The midgut of adult female Anopheles aquasalis presents a narrow anterior or thoracic region and a distensible posterior or abdominal region constituted by the epithelium formed by a cell layer whose apical portion presents microvilli and the basal portion, a basal labyrinth. The thoracic region revealed heterogeneous cellular staining affinity mainly by the presence of acidic components. The ultrastructural aspect showed columnar cells with the presence of the vesicle, mitochondria, endoplasmic reticulum and secreting cells. The abdominal region of the midgut revealed an irregular epithelium whose cells presented a basophilic cytoplasm and acidophil granules. It was also found secreting and/or basal cells with narrow cytoplasm. The ultrastructural observation of this region demonstrated cells with evident nucleus, endoplasmic reticulum and mitochondria. Larger vesicles and small granules were found distributed throughout the cytoplasm. The basal lamina that supports the epithelium presented a generally irregular aspect and the muscle fibers have longitudinal and circular organization and were found separating the epithelium from the haemocel. This study will contribute to analyses on the vector mosquito-parasite interaction mechanism in this specimen.

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“…Successes with An. darlingi colonization [162][163][164] and genome sequence and annotation [165] and similar advances with other neotropical anophelines [166][167][168][169] offer optimism that these technologies will soon be transferred to neotropical Amazonian malaria vectors.…”

Section: Challenges Alternatives and Transfer To Neotropical Speciesmentioning

confidence: 99%

Vector-Focused Approaches to Curb Malaria Transmission in the Brazilian Amazon: An Overview of Current and Future Challenges and Strategies

et al. 2020

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In Brazil, malaria transmission is mostly confined to the Amazon, where substantial progress has been made towards disease control in the past decade. Vector control has been historically considered a fundamental part of the main malaria control programs implemented in Brazil. However, the conventional vector-control tools have been insufficient to control or eliminate local vector populations due to the complexity of the Amazonian rainforest environment and ecological features of malaria vector species in the Amazon, especially Anopheles darlingi. Malaria elimination in Brazil and worldwide eradication will require a combination of conventional and new approaches that takes into account the regional specificities of vector populations and malaria transmission dynamics. Here we present an overview on both conventional and novel promising vector-focused tools to curb malaria transmission in the Brazilian Amazon. If well designed and employed, vector-based approaches may improve the implementation of malaria-control programs, particularly in remote or difficult-to-access areas and in regions where existing interventions have been unable to eliminate disease transmission. However, much effort still has to be put into research expanding the knowledge of neotropical malaria vectors to set the steppingstones for the optimization of conventional and development of innovative vector-control tools.

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Laboratory Colonization of Anopheles aquasalis (Diptera: Culicidae) in Belém, Pará, Brazil

Cited by 14 publications

References 10 publications

Description of an automatic copulation induction system used to establish a free-mating laboratory colony of Nyssorhynchus deaneorum from Brazil

Description of an automatic copulation induction system used to establish a free-mating laboratory colony of Nyssorhynchus deaneorum from Brazil

Morphological Aspect of the Midgut of Anopheles aquasalis (Curry, 1932) Insecta: Diptera

Vector-Focused Approaches to Curb Malaria Transmission in the Brazilian Amazon: An Overview of Current and Future Challenges and Strategies

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