Laboratory diagnosis of endophthalmitis: Comparison of microbiology and molecular methods in the European Society of Cataract & Refractive Surgeons multicenter study and susceptibility testing

Abstract

Use of molecular biology technique increased the laboratory rate of identifying the pathogen by 20%, confirming the technique is very useful for the endophthalmitis specimen. Samples of both aqueous and vitreous should be collected and stored at -20 degrees C for PCR at the time of the diagnostic taps.

Intracameral antibiotic prophylaxis: American paper mirrors European experience

“…3 The Shorstein et al study had 5 staphylococcal and 3 streptococcal infections in the nonintracameral group but no culture-positive cases in patients receiving the injection.…”

Endophthalmitis Following Cataract Surgery: Clinical Features, Treatment and Prophylaxis

“…The presumed source of the causative organism in the EVS and ESCRS Study was the ocular surface of study patients (Bannerman et al, 1997;Seal et al, 2008). In a systematic review of all prophylactic methods for cataract surgery, instillation of 5% povidone-iodine on the conjunctiva 3 minutes before the surgery received the highest rating and was the only measure that gained a Grade B rating based on evidence from randomized clinical trials (Ciulla et al, 2002).…”

Diagnostic dilemmas in retinitis and endophthalmitis

“…2,[29][30][31][32][33][34] Concordance with culture is close to 100%, with greater sensitivity with PCR testing. 29,34 The presumption is that PCR will eventually replace culture and sensitivity testing (by amplifying loci known to determine resistance to antibiotics 35 ) and enable the detection of unsuspected, fastidious, or previously unknown pathogens. 9,36,37 Intraocular Whipple disease is diagnosable by PCR of aqueous humor or vitreous.…”