Laboratory diagnosis of Schistosomiasis in areas of low transmission: a review of a line of research

Noya, Óscar; Noya, B Alarcón de; Losada, Sandra; Colmenares, Cecilia; Guzmán, C; Lorenzo, Marı́a M.; Bermúdez, Henry

doi:10.1590/s0074-02762002000900032

Cited by 45 publications

(29 citation statements)

References 19 publications

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“…Soluble WWA preparations of S. mansoni at a concentration of 250 ng/dot were also reported to yield false positive results in malaria and filaria positive human subjects (Montenegro et al 1999). Noya et al (2002) had also observed false positive reactions in western blot and ELISA with crude WWA and egg antigens of S. mansoni particularly in individuals infected with nematodes, though these antigens possessed high sensitivity for the diagnosis of infection. In the present study S. spindale ESA had not exhibited cross reaction with amphistome positive sera in Dot-ELISA.…”

Section: Discussionmentioning

confidence: 99%

Efficacy of Dot-ELISA using different antigens in detecting anti-schistosome antibodies among bovines in field conditions

et al. 2014

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Schistosomosis has been recognised as one of the major parasitic diseases of livestock and human beings. Schistosoma spindale is the major cause of visceral schistosomosis among bovines of Kerala State. Besides pathology in animals, it has been long known that cercariae of S. spindale are a common cause of dermatitis in human beings in Asia. However, detection of this disease based on coprology has underestimated the prevalence of this economically important disease among cattle of the State. An efficient diagnostic tool providing unequivocal evidence of infection in living animals is perhaps, the key to formulate and deliver control measures to the target population. It is also crucial for an enhanced understanding of parasite epidemiology. The utility of excretory-secretory proteins as diagnostic and vaccine candidates for schistosomosis has been a focus of medical research since long. There exists a paucity of information with regard to analysis of ES proteins of S. spindale and their incorporation to develop sensitive and specific serodiagnostic tool. Hence a study was designed to evaluate the efficacy of Dot-ELISA incorporating different antigens of S. spindale and to validate the test under field conditions.

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Section: Discussionmentioning

confidence: 99%

Efficacy of Dot-ELISA using different antigens in detecting anti-schistosome antibodies among bovines in field conditions

et al. 2014

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“…For instance, in seroepidemiological surveys, apart from test procedures, sample collection and processing are sometimes cumbersome and require trained personnel for venopuncture and separation of serum samples under field conditions. Besides that, venopuncture is not widely accepted, especially by children and certain ethnic and religious groups (Noya et al, 2002). For field-based diagnosis and follow-up of chemotherapy of schistosomiasis, there is urgent need for simple and reliable tests, which are applicable in endemic areas (van Lieshout et al, 1992(van Lieshout et al, , 2000Chitsulo et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of circulating cathodic antigen (CCA) strip for diagnosis of urinary schistosomiasis in Hassoba school children, Afar, Ethiopia

Ayele

Erko²,

Legesse³

et al. 2008

Parasite

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Summary :A total of 206 urine samples collected from Hassoba Elementary schoolchildren, Afar, Ethiopia, a low Schistosoma haematobium endemic setting, was diagnosed to evaluate the performance of CCA strip using double references, urine filtration technique and urinalysis dipstick (Combur 10 Test ® ) that detect schistosome eggs and blood in urine, respectively. The former was used as a gold standard reference method. Sensitivity, specificity, positive and negative predictive values for the CCA were 52 %, 63.8 %, 56.7 % and 59 % respectively, with reference to urine filtration technique whereas these parameters were 50.4 %, 62.4 %, 55.6 % and 57.5 % respectively, with reference to Combur 10 Test ® . 47 S. haematobium egg-positive children were found negative by CCA strip while 38 egg-negative children were found positive by CCA strip. Moreover, among the pre-tests done in duplicate, inconsistent results were also recorded. Assays were also compared with regard to the cost of equipment and reagents, speed and simplicity of use. Though CCA strip was found to be rapid and could be performed with minimal training, it was found to be expensive (US $ 4.95 per test) to use it for large-scale field use even if its diagnostic value would have been satisfactory. Further development and standardization of the CCA strip are required for its applicability for field use. It is also recommended that its cost per strip should be substantially cut down if it is to be used in poor schistosomiasis endemic countries. Résumé

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“…For field work, the whole population can be screened though ELISA-SEA-SMP or APIA. Then, Kato-Katz, and COPT would be applied only to the positives as proposed by Noya et al (2002). An exam with the advantages of the ELISA-like test that discriminates active from past infection would be very useful when evaluation of the impact of treatment is the goal.…”

Section: Schistosomiasis Applied Diagnosis In Venezuelamentioning

confidence: 99%

“…Based on the fact that morbidity studies in these areas really do not help too much, laboratorial markers of active infection have been taken into consideration to define cases of schistosomiasis. We have proposed three categories (Alarcón de Noya et al 2002):…”

Section: Schistosomiasis Case Definitionmentioning

confidence: 99%

“…This fact could be responsible for "false positives" detected by serological assays, since, they do not represent a problem in terms of pathology and epidemiology of this infection; by the contrary, these individuals could be more resistant to re-infections, helping to maintain a lower prevalence in these areas. The presence of snails allowed us to better describe the kind of focus we were dealing with (Alarcón de Noya et al 2002). Low transmission area is not a static concept.…”

Section: Malacologymentioning

confidence: 99%

See 1 more Smart Citation

Low transmission areas of schistosomiasis in Venezuela: consequences on the diagnosis, treatment, and control

Noya¹,

Guevara²,

Colmenares³

et al. 2006

Mem. Inst. Oswaldo Cruz

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Laboratory diagnosis of Schistosomiasis in areas of low transmission: a review of a line of research

Cited by 45 publications

References 19 publications

Efficacy of Dot-ELISA using different antigens in detecting anti-schistosome antibodies among bovines in field conditions

Efficacy of Dot-ELISA using different antigens in detecting anti-schistosome antibodies among bovines in field conditions

Evaluation of circulating cathodic antigen (CCA) strip for diagnosis of urinary schistosomiasis in Hassoba school children, Afar, Ethiopia

Low transmission areas of schistosomiasis in Venezuela: consequences on the diagnosis, treatment, and control

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