Laboratory Efforts to Improve Motility of Human Sperm

Lessing, Joseph B.; G, Paz; Peyser, M. Reuben; Homonnai, Z. T.; Brenner, Sara; Weiss, Gerson

doi:10.1055/s-2007-1021856

Cited by 2 publications

(1 citation statement)

References 37 publications

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“…The first sperm recovery procedure used for human IVF was a simple one-step washing (Edwards et al, 1969). More recently, various techniques have been proposed to improve the yield (Lessing et al, 1987); swim-up and Percoll gradient are the most widespread. However, in severe oligoasthenozoospermia, recovery of motile spermatozoa is often poor and leads to failure of IVF.…”

Section: Total"mentioning

confidence: 99%

In-vitro fertilization in cases with severe sperm defect: use of a swim-across technique and medium supplemented with follicular fluid

Giorgetti¹,

Hans²,

Spach³

et al. 1992

Human Reproduction

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The purpose of this study was to evaluate a new method of in-vitro fertilization (IVF) in patients with severe sperm defects. Unlike the conventional swim-up method, spermatozoa and oocytes are placed in opposite corners of the bottom of the incubation dish so that sperm swimming is horizontal instead of vertical. Another difference between the swim-across and swim-up techniques is that the incubation medium is supplemented with 20% follicular fluid. After a randomized series (protocol I) of 15 IVF attempts had demonstrated that swim-across was more effective than swim-up in terms of fertilization and cleavage, we began a second series (protocol II) using only swim-across. A total of 124 couples with motile sperm counts less than 1 x 10(6) spermatozoa/ml of semen were included in protocol II. Clinical parameters (age, tubal damage) and number of recovered oocytes were recorded and compared in patients who did (group A: n = 94) and did not (group B: n = 74) achieve fertilization. In group A the fertilization rate was 36.7% and, out of the 94 transfers that were made, there were 21 clinical pregnancies and 12 full-term pregnancies with 16 live births. The number of oocytes collected (12 versus 7.7, P less than 0.001) and the incidence of tubal damage (50% versus 24.3%, P less than 0.001) was significantly higher in group A than in group B. Using logistic regression analysis, we showed a significant correlation between fertilization and progressive motility, percentage normal spermatozoa, number of recovered oocytes and tubal damage.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Total"mentioning

confidence: 99%