Laboratory evaluation of a two-stage treatment system for TCE cometabolism by a methane-oxidizing mixed culture

Smith, Laurence; McCarty, Perry L.

doi:10.1002/(sici)1097-0290(19970820)55:4<650::aid-bit7>3.0.co;2-g

Cited by 24 publications

(6 citation statements)

References 43 publications

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“…Hence, we observed a conflict between providing sufficient methane to support methanotrophic growth and securing sufficient quantities of MMO to degrade the pesticides, and the inhibitory effects of the presence of methane. Similar challenges are reported for methanotrophic bioremediation of contaminants in other systems (Jiang et al, 2010;Sullivan et al, 1998), and have been partially overcome by the use of sequential systems where growth and co-metabolic biodegradation takes place in different reactors (Jiang et al, 2010;Smith and McCarty, 1997).…”

Section: Challenges For the Application Of Methanotrophic Column Reacmentioning

confidence: 66%

Methanotrophic contribution to biodegradation of phenoxy acids in cultures enriched from a groundwater-fed rapid sand filter

Papadopoulou

Hedegaard

Dechesne

et al. 2018

Appl Microbiol Biotechnol

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Section: Challenges For the Application Of Methanotrophic Column Reacmentioning

confidence: 66%

Methanotrophic contribution to biodegradation of phenoxy acids in cultures enriched from a groundwater-fed rapid sand filter

Papadopoulou

Hedegaard

Dechesne

et al. 2018

Appl Microbiol Biotechnol

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“…For example, Engelen et al (1998) report that the long-term exposition of a bacterial community to a paraffin oil named Oleo determined the marked selection of one strain; Watts et al (2001) found that the degradation of the PCB congener 2,3,4,5-chlorobiphenyl led to the selection of four strains; Shinoda et al (2000) report that microbial acclimation was still occurring after 3 years of phenol biodegradation. A relevant consequence of these observations is that, as the bacterial population changes, also the parameters estimated from the results relative to one reactor are likely to change progressively: for example, in a 7-year study of TCE degradation by a methane-utilizing mixed culture (Smith & McCarty 1997), the authors observed remarkable increases of the transformation yield and of the transformation capacity. It is therefore important to operate labscale studies of CAH biodegradation for prolonged periods, so as to obtain long-term estimates of the main parameters characterizing the process and to select bacterial strains potentially utilizable in bioaugmentation treatments.…”

Section: Introductionmentioning

confidence: 99%

Aerobic cometabolism of chloroform by butane-grown microorganisms: long-term monitoring of depletion rates and isolation of a high-performing strain

et al. 2005

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The focus of this microcosm study was to monitor the performances of 17 butane-utilizing microcosms during a long-term (100-250 days) aerobic cometabolic depletion of chloroform (CF). The depletion of the contaminant began after a lag-time variable between 0 and 23 days. All microcosms quickly reached a pseudo steady-state condition, in terms of biomass concentration (with an average of 9.3 x 106 CFU ml(-1)), chloroform depletion rate (5 micromol l(-1) d(-1)) and butane utilization rate (730 micromol l(-1) d(-1)). After about 100 days of CF depletion, a sudden 5- to 7-fold increase of the chloroform rate was observed in two microcosms, where the highest amount of contaminant had been depleted. In one of these high-performing microcosms, an experiment of chloroform depletion in the absence of butane resulted in the depletion of a surprisingly high amount of contaminant (765 micromolCF kg(-1) dry soil in 2 months) and in a marked selection of a single bacterial strain. Bioaugmentation assays conducted with the biomass selected in this microcosm and with a pure culture of the selected strain immediately resulted in very high chloroform depletion rates. Preliminary results of a study conducted with resting cells of the selected strain indicated that it can degrade chloroform concentrations up to 119 microM (14.2 mg l(-1)) without any sign of substrate toxicity, and that it is able to transform vinyl chloride and 1,1,2-trichloroethane.

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“…Degradation-mediated toxicity is attributed to breakdown products (e.g. TCE epoxides) attacking cellular components including oxygenase enzymes (Smith & McCarty 1997;Sun & Wood 1997) and is proportional to the extent of TCE co-metabolism (Alvarez-Cohen & McCarty 1991). Interestingly, standards 10 and 17 (Flavobacterium sp.…”

Section: Discussionmentioning

confidence: 99%

Changes in soil microbial community composition induced by cometabolism of toluene and trichloroethylene

2005

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The effects of trichloroethylene (TCE) on microbial community composition were analyzed by reverse sample genome probing. Soil enrichments were incubated in dessicators containing an organic phase of either 1 or 10% (w/w) toluene in vacuum pump oil, delivering constant equilibrium aqueous concentrations of 16 and 143 mg/l, respectively. Increasing the equilibrium aqueous concentration of TCE from 0 to 10 mg/l led to shifts in community composition at 16, but not at 143 mg/l of toluene. In closed system co-degradation studies, TCE at an aqueous concentration of 1 mg/l was effectively degraded by toluene-degrading soil enrichments once the aqueous toluene concentration dropped below 25 mg/l. Little TCE degradation was observed at higher toluene concentrations (50-250 mg/l). The results indicate that TCE changes microbial community composition under conditions where it is being actively metabolized.

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Laboratory evaluation of a two-stage treatment system for TCE cometabolism by a methane-oxidizing mixed culture

Cited by 24 publications

References 43 publications

Methanotrophic contribution to biodegradation of phenoxy acids in cultures enriched from a groundwater-fed rapid sand filter

Methanotrophic contribution to biodegradation of phenoxy acids in cultures enriched from a groundwater-fed rapid sand filter

Aerobic cometabolism of chloroform by butane-grown microorganisms: long-term monitoring of depletion rates and isolation of a high-performing strain

Changes in soil microbial community composition induced by cometabolism of toluene and trichloroethylene

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