Laboratory evaluation of an outbreak of nocardiosis in immunocompromised hosts

Stevens, David A.; Pier, A. C.; Beaman, Blaine L.; Morozumi, Pius A.; Lovett, I.S.; Houang, Elizabeth T.

doi:10.1016/0002-9343(81)90302-8

Cited by 65 publications

(17 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The present results confirm that plasmids do not seem to be lost readily during laboratory cultivation and maintenance [3]. For this reason, when plasmid( s) is(are) present, plasmid analysis could be a means of strain comparison in an epidemiological evaluation of outbreaks of nocardial infections [24] or in recurrence of infection in an individual case [23].…”

Section: Discussionsupporting

confidence: 77%

Occurrence of plasmids in pathogenic strains of Nocardia

Provost

Blanc²,

Beaman³

et al. 1996

Journal of Medical Microbiology

View full text Add to dashboard Cite

The purpose of the present study was to determine the relative distribution of plasmids in 87 clinical isolates of Nucardia, belonging to the five major pathogenic species. A correlation between plasmid content and the site of infection within the host, resistance to antibiotics and enzymic profiles was also investigated. The plasmid extraction procedure of Kado and Liu was used. Electrophoretic analysis revealed one-to-four plasmid bands, ranging in size from <8 to >50 kb, in 27 strains (31%). Based on the number of isolates tested, the incidence of plasmid-bearing strains was significantly higher among N. farcinica than N. asteroides strains. Within N. farcinica, the incidence of plasmids was higher among strains isolated in the Paris area than in strains isolated elsewhere, such as in the French provinces or outside France. A statistically significant correlation was demonstrated between the cutaneous localisation of infections and the incidence of plasmid-bearing strains. The presence of plasmids in nocardiae could not be associated with specific phenotypic traits such as resistance to antibiotics or enzymic activity. The fact that the majority of Nocardia clinical isolates (60 of 87) did not contain plasmids suggests that plasmids are not involved directly in virulence and that there is no selective pressure for plasmid acquisition.

show abstract

Section: Discussionsupporting

confidence: 77%

Occurrence of plasmids in pathogenic strains of Nocardia

Provost

Blanc²,

Beaman³

et al. 1996

Journal of Medical Microbiology

View full text Add to dashboard Cite

show abstract

“…Stevens et al showed that the level of antibody response seemed to be related to the stage of the infection, with higher titers seen early in the disease or, in one case, before the appearance of symptoms (184).…”

Section: Identification Procedures (I) Biochemical Methodsmentioning

confidence: 99%

Clinical and Laboratory Features of theNocardiaspp. Based on Current Molecular Taxonomy

et al. 2006

View full text Add to dashboard Cite

SUMMARY The recent explosion of newly described species of Nocardia results from the impact in the last decade of newer molecular technology, including PCR restriction enzyme analysis and 16S rRNA sequencing. These molecular techniques have revolutionized the identification of the nocardiae by providing rapid and accurate identification of recognized nocardiae and, at the same time, revealing new species and a number of yet-to-be-described species. There are currently more than 30 species of nocardiae of human clinical significance, with the majority of isolates being N. nova complex, N. abscessus, N. transvalensis complex, N. farcinica, N. asteroides type VI (N. cyriacigeorgica), and N. brasiliensis. These species cause a wide variety of diseases and have variable drug susceptibilities. Accurate identification often requires referral to a reference laboratory with molecular capabilities, as many newer species are genetically distinct from established species yet have few or no distinguishing phenotypic characteristics. Correct identification is important in deciding the clinical relevance of a species and in the clinical management and treatment of patients with nocardial disease. This review characterizes the currently known pathogenic species of Nocardia, including clinical disease, drug susceptibility, and methods of identification.

show abstract

“…The lack of humoral response is in agreement with the findings of Krick and Remington (1 975) and Conde et al (1 983). A few studies have, however, detected a very low titre of antibody in nocardiosis in man (Stevens et al, 1981) and in cattle (Pier et al, 1968), and in experimentally induced local infection in guinea pigs (Hiramine et al, 1981) against a whole-cell extract of N . asteroides.…”

Section: Discussionmentioning

confidence: 99%