Laboratory evaluation of Fendona 6SC® treated bednets and Interceptor® long-lasting nets against Anopheles gambiae s.l. in Burkina Faso

Badolo, Athanase; Guelbéogo, Wamdaogo Moussa; Tiono, Alfred B.; Traoré, Abdoulaye; Sagnon, N’Falé; Sirima, Sodiomon B.

doi:10.1007/s00436-013-3742-3

Cited by 9 publications

(7 citation statements)

References 40 publications

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“…fluviatilis was greatly deterred from entering the huts and those mosquitoes that entered the huts were prevented from feeding on the host, despite the presence of holes on the nets, indicating a signiÞcant and comparable personal protection offered by the LNs and SPs. Different results were obtained for vectorsÕ exiting in response to the SPs and LNs when used in experimental huts; some reported induced exophily (Badolo et al 2012, Malima et al 2013) and others observed insigniÞcant difference relative to the control (Mosha et al 2008). Such difference depends also on the innate characteristic of the targeted vector species.…”

Section: Discussionmentioning

confidence: 99%

“…However, Corbel et al (2004) observed a higher deterrence (83Ð 89%) against Anopheles gambiae with nets treated with 50, 100, 250, and 500 mg permethrin/ m 2 . But, against the same vector species, there was no clear evidence of deterred effect of alphacypermethrin-treated (Interceptor) and deltamethrin-treated (PermaNet) LNs (Malima et al 2013, Badolo et al 2012. In an experimental hut trial of bed-nets impregnated with SPs or organophosphate (OP) insecticide for mosquito control in The Gambia, it was concluded that permethrin tends mainly to deter mosquitoes from house entry, enhancing personal protection, whereas the OP insecticide kills higher proportions of the endophilic mosquitoes, which would give better community protection against malaria transmission (Miller et al 1991).…”

Section: Discussionmentioning

confidence: 99%

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Comparison of Efficacy of Five Types of Long-Lasting Insecticidal Nets AgainstAnopheles fluviatilis, the Primary Malaria Vector in East-Central India

et al. 2014

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Five types of long-lasting insecticidal nets (LNs), namely, Olyset, Netprotect, PermaNet, DuraNet, and Interceptor, were tested after 20 washes for efficacy in terms of mortality, deterrence effect, blood-feeding inhibition, and induced exophily of the malaria vector Anopheles fluviatilis in experimental huts in Malkangiri district of Odisha State, India. Efficacy of the three synthetic pyrethroids (SPs) used in the LNs was also analyzed. Use of LNs reduced the entry of An. fluviatilis into the huts by 73.3-83.2%, and the five LNs were comparable in terms of deterrence. The exit rate of An. fluviatilis from the huts with untreated net was 56.3%, and relative to this, Olyset followed by DuraNet induced significantly a higher exophily. In contrast, the exit rate was significantly lower with Interceptor. Among the three SPs, permethrin induced significantly greater exophily relative to the untreated control, and as a result of this, permethrin-treated Olyset produced a lower mortality. Blood-feeding rate of An. fluviatilis was significantly lower with all the five LNs than the control. Similarly, all the three SPs significantly inhibited blood feeding compared with the control. Interceptor and DuraNet, both alphacypermethrin-treated LNs, caused relatively a higher mortality of An. fluviatilis than the other LNs. The five brands of LNs and three SPs tested in the current study were equally effective in terms of deterrence and blood-feeding inhibition; only exiting and killing effect differed among them. Permethrin-treated LNs induced greater exophily, while, overall, alphacypermethrin-treated LNs killed more An. fluviatilis that entered the huts. Advantage of deterrence, excito-repellent, and killing effects of LNs and appropriate selection of SP for net treatment are discussed in this paper.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Comparison of Efficacy of Five Types of Long-Lasting Insecticidal Nets AgainstAnopheles fluviatilis, the Primary Malaria Vector in East-Central India

et al. 2014

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“…To calculate the proportion of each entomological outcomes and the level of significance between the treatments and between the control for each entomological outcomes, the XLSTAT software (Addinsoft, Berkeley, CA, USA) was used, as done previously [29,30]. The numbers of mosquitoes collected in the huts with different treatments were analysed by negative binomial regression.…”

Section: Experimental Hut Trialmentioning

confidence: 99%

An Experimental Hut Evaluation of PBO-Based and Pyrethroid-Only Nets against the Malaria Vector Anopheles funestus Reveals a Loss of Bed Nets Efficacy Associated with GSTe2 Metabolic Resistance

Menze

Kouamo

Wondji

et al. 2020

Genes

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Growing insecticide resistance in malaria vectors is threatening the effectiveness of insecticide-based interventions, including Long Lasting Insecticidal Nets (LLINs). However, the impact of metabolic resistance on the effectiveness of these tools remains poorly characterized. Using experimental hut trials and genotyping of a glutathione S-transferase resistance marker (L119F-GSTe2), we established that GST-mediated resistance is reducing the efficacy of LLINs against Anopheles funestus. Hut trials performed in Cameroon revealed that Piperonyl butoxide (PBO)-based nets induced a significantly higher mortality against pyrethroid resistant An. funestus than pyrethroid-only nets. Blood feeding rate and deterrence were significantly higher in all LLINs than control. Genotyping the L119F-GSTe2 mutation revealed that, for permethrin-based nets, 119F-GSTe2 resistant mosquitoes have a greater ability to blood feed than susceptible while the opposite effect is observed for deltamethrin-based nets. For Olyset Plus, a significant association with exophily was observed in resistant mosquitoes (OR = 11.7; p < 0.01). Furthermore, GSTe2-resistant mosquitoes (cone assays) significantly survived with PermaNet 2.0 (OR = 2.1; p < 0.01) and PermaNet 3.0 (side) (OR = 30.1; p < 0.001) but not for Olyset Plus. This study shows that the efficacy of PBO-based nets (e.g., blood feeding inhibition) against pyrethroid resistant malaria vectors could be impacted by other mechanisms including GST-mediated metabolic resistance not affected by the synergistic action of PBO. Mosaic LLINs incorporating a GST inhibitor (diethyl maleate) could help improve their efficacy in areas of GST-mediated resistance.

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“…Experimental hut trial: To calculate the proportion of each entomological outcomes and the level of significance between the treatments and between the control for each entomological outcomes, the XLSTAT software was used, as before. The confidence limits of these parameters were calculated and the proportions were compared using a chi-square test with a significance limit of 0.05 [31,43,50,51].…”

Section: Discussionmentioning

confidence: 99%

Experimental Hut Trials Reveal That CYP6P9a/b P450 Alleles Are Reducing the Efficacy of Pyrethroid-Only Olyset Net against the Malaria Vector Anopheles funestus but PBO-Based Olyset Plus Net Remains Effective

et al. 2022

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Malaria remains a major public health concern in Africa. Metabolic resistance in major malaria vectors such as An. funestus is jeopardizing the effectiveness of long-lasting insecticidal nets (LLINs) to control malaria. Here, we used experimental hut trials (EHTs) to investigate the impact of cytochrome P450-based resistance on the efficacy of PBO-based net (Olyset Plus) compared to a permethrin-only net (Olyset), revealing a greater loss of efficacy for the latter. EHT performed with progenies of F5 crossing between the An. funestus pyrethroid-resistant strain FUMOZ and the pyrethroid-susceptible strain FANG revealed that PBO-based nets (Olyset Plus) induced a significantly higher mortality rate (99.1%) than pyrethroid-only nets (Olyset) (56.7%) (p < 0.0001). The blood-feeding rate was higher in Olyset compared to Olyset Plus (11.6% vs. 5.6%; p = 0.013). Genotyping the CYP6P9a/b and the intergenic 6.5 kb structural variant (SV) resistance alleles showed that, for both nets, homozygote-resistant mosquitoes have a greater ability to blood-feed than the susceptible mosquitoes. Homozygote-resistant genotypes significantly survived more with Olyset after cone assays (e.g., CYP6P9a OR = 34.6; p < 0.0001) than homozygote-susceptible mosquitoes. A similar but lower correlation was seen with Olyset Plus (OR = 6.4; p < 0.001). Genotyping EHT samples confirmed that CYP6P9a/b and 6.5 kb_SV homozygote-resistant mosquitoes survive and blood-feed significantly better than homozygote-susceptible mosquitoes when exposed to Olyset. Our findings highlight the negative impact of P450-based resistance on pyrethroid-only nets, further supporting that PBO nets, such as Olyset Plus, are a better solution in areas of P450-mediated resistance to pyrethroids.

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Laboratory evaluation of Fendona 6SC® treated bednets and Interceptor® long-lasting nets against Anopheles gambiae s.l. in Burkina Faso

Cited by 9 publications

References 40 publications

Comparison of Efficacy of Five Types of Long-Lasting Insecticidal Nets AgainstAnopheles fluviatilis, the Primary Malaria Vector in East-Central India

Comparison of Efficacy of Five Types of Long-Lasting Insecticidal Nets AgainstAnopheles fluviatilis, the Primary Malaria Vector in East-Central India

An Experimental Hut Evaluation of PBO-Based and Pyrethroid-Only Nets against the Malaria Vector Anopheles funestus Reveals a Loss of Bed Nets Efficacy Associated with GSTe2 Metabolic Resistance

Experimental Hut Trials Reveal That CYP6P9a/b P450 Alleles Are Reducing the Efficacy of Pyrethroid-Only Olyset Net against the Malaria Vector Anopheles funestus but PBO-Based Olyset Plus Net Remains Effective

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