Laboratory Evaluation of Isaria fumosorosea CCM 8367 and Steinernema feltiae Ustinov against Immature Stages of the Colorado Potato Beetle

Hussein, Hany M.; Habuštová, Oxana Skoková; Půža, Vladimı́r; Zemek, Rostislav

doi:10.1371/journal.pone.0152399

Cited by 28 publications

(32 citation statements)

References 63 publications

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“…Choo et al [65] also reported that the combination of S. carpocap1ae (Weiser) with B. brongniartii (Saccardo) Petch resulted in a significant increase in the mortality of oriental beetle, Exomala orientalis (Waterhouse) grubs compared to the application of fungus alone. Most recently the combined application of the fungus Ifr CCM 8367 with entomopathogenic nematodes increased the mortality of the Colorado potato beetle larva up to 98.0% compared to either entomopathogen applied alone [66]. Jabbour et al [67] concluded after investigating entomopathogen biodiversity and its effect on host mortality, that the combined pairings of fungal entomopathogens and entomopathogenic nematodes always produced host mortality that exceeded predictions based on the impact of either entomopathogen alone.…”

Section: Discussionmentioning

confidence: 99%

Efficacy of Topical Application, Leaf Residue or Soil Drench of Blastospores of Isaria fumosorosea for Citrus Root Weevil Management: Laboratory and Greenhouse Investigations

Avery

Hunter

Hall

et al. 2016

Insects

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The efficacy of topical, leaf residue, and soil drench applications with Isaria fumosorosea blastospores (Ifr strain 3581) was assessed for the management of the citrus root weevil, Diaprepes abbreviatus (L.). Blastospores of Ifr were applied topically at a rate of 107 blastospores mL−1 on both the larvae and adults, and each insect stage was incubated in rearing cups with artificial diet at 25 °C, either in the dark or in a growth chamber under a 16 h photophase for 2 weeks, respectively. Percent larval and adult mortality due to the infection of Ifr was assessed after 14 days as compared to untreated controls. Leaf residue assays were assessed by feeding the adults detached citrus leaves previously sprayed with Ifr (107 blastospores mL−1) in Petri dish chambers and then incubating them at 25 °C for 2–3 weeks. Efficacy of the soil drench applications was assessed on five larvae feeding on the roots of a Carrizo hybrid citrus seedling ~8.5–10.5 cm below the sterile sand surface in a single 16 cm × 15.5 cm pot inside a second pot lined with plastic mesh to prevent escapees. Drench treatments per pot consisted of 100 mL of Ifr suspension (107 blastospores mL−1), flushed with 400, 900, or 1400 mL of water compared to 500, 1000, and 1500 mL of water only for controls. The mean concentration of Ifr propagules as colony forming units per gram (CFUs g−1) that leached to different depths in the sand profile per treatment drench rate was also determined. Two weeks post-drenching of Ifr treatments, larvae were assessed for percent mortality, size differences, and effect of treatments in reducing feeding damage to the plant root biomass compared to the controls. Topical spray applications caused 13 and 19% mortality in larvae and adults after 7 days compared to none in the control after 14 days, respectively. Adults feeding on a single Ifr treated leaf for 24 h consumed less than the control, and resulted in 100% mortality 35 days post-treatment compared to 33% in the untreated control. Although offered fresh, untreated leaves after 24 h, only adults in the control group consumed them. Ifr CFUs g−1 were isolated 8.5–10.5 cm below the sand surface for the 1000 and 1500 mL drench rates only, resulting in 2%–4% larval mortality. For all the Ifr drench treatments, no differences were observed in percent larval mortality and size or the effect of treatments in reducing feeding damage to the plant root biomass compared to the controls. These results suggest that the foliar application of Ifr may be an efficient biocontrol strategy for managing adult populations of D. abbreviatus; potential alternative larval management strategies are discussed.

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Section: Discussionmentioning

confidence: 99%

Efficacy of Topical Application, Leaf Residue or Soil Drench of Blastospores of Isaria fumosorosea for Citrus Root Weevil Management: Laboratory and Greenhouse Investigations

Avery

Hunter

Hall

et al. 2016

Insects

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“…P. fumosoroseus is capable of affecting whitefly Aleurodicus cocois at various developmental stages [ 234 ]. Similarly, the physical barriers of Leptinotarsa decemlineata pupae make them more resistant than their larvae to Isaria fumosorosea infection [ 235 ]. In terms of size, Hunter et al [ 236 ] showed a negative correlation between insect mortality and size mainly because larger sizes are associated with thicker cuticles, as in the use of I. fumosorosea on Diaphorina citri (psyllid) and D. citri (curculionidae), in which case the latter is bigger.…”

Section: Biological Control Of Diseases Caused By Arthropodsmentioning

confidence: 99%

“…Numerous orders of arthropods that are vulnerable to the use of Paecilomyces spp. ( Table 3 ), including hemiptera, have been described, such as aleurodids [ 246 ], aphids [ 238 ], thysanoptera [ 242 ], diptera [ 247 ], lepidoptera [ 73 ], hymenoptera [ 248 ] and coleoptera [ 235 ].…”

Section: Biological Control Of Diseases Caused By Arthropodsmentioning

confidence: 99%

Paecilomyces and Its Importance in the Biological Control of Agricultural Pests and Diseases

Moreno-Gavíra

Huertas

Diánez

et al. 2020

Plants

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Incorporating beneficial microorganisms in crop production is the most promising strategy for maintaining agricultural productivity and reducing the use of inorganic fertilizers, herbicides, and pesticides. Numerous microorganisms have been described in the literature as biological control agents for pests and diseases, although some have not yet been commercialised due to their lack of viability or efficacy in different crops. Paecilomyces is a cosmopolitan fungus that is mainly known for its nematophagous capacity, but it has also been reported as an insect parasite and biological control agent of several fungi and phytopathogenic bacteria through different mechanisms of action. In addition, species of this genus have recently been described as biostimulants of plant growth and crop yield. This review includes all the information on the genus Paecilomyces as a biological control agent for pests and diseases. Its growth rate and high spore production rate in numerous substrates ensures the production of viable, affordable, and efficient commercial formulations for agricultural use.

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“…In an interesting manner, additive or synergistic interactions of the control agent with other species can be exploited for biological control. However, the nature of their interaction (antagonistic or additive/synergistic) depends on, for example, timing (simultaneously or sequential) and rate of application (Hussein, Habustova, Puza, & Zemek, ; Shapiro‐Ilan et al., ).…”

Section: Step 3: Understanding Variation In D Suzukii–parasitoid Intmentioning

confidence: 99%

Optimization of native biocontrol agents, with parasitoids of the invasive pest Drosophila suzukii as an example

Kruitwagen

Beukeboom

Wertheim

2018

Evolutionary Applications

115

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The development of biological control methods for exotic invasive pest species has become more challenging during the last decade. Compared to indigenous natural enemies, species from the pest area of origin are often more efficient due to their long coevolutionary history with the pest. The import of these well‐adapted exotic species, however, has become restricted under the Nagoya Protocol on Access and Benefit Sharing, reducing the number of available biocontrol candidates. Finding new agents and ways to improve important traits for control agents (“biocontrol traits”) is therefore of crucial importance. Here, we demonstrate the potential of a surprisingly under‐rated method for improvement of biocontrol: the exploitation of intraspecific variation in biocontrol traits, for example, by selective breeding. We propose a four‐step approach to investigate the potential of this method: investigation of the amount of (a) inter‐ and (b) intraspecific variation for biocontrol traits, (c) determination of the environmental and genetic factors shaping this variation, and (d) exploitation of this variation in breeding programs. We illustrate this approach with a case study on parasitoids of Drosophila suzukii, a highly invasive pest species in Europe and North America. We review all known parasitoids of D. suzukii and find large variation among and within species in their ability to kill this fly. We then consider which genetic and environmental factors shape the interaction between D. suzukii and its parasitoids to explain this variation. Insight into the causes of variation informs us on how and to what extent candidate agents can be improved. Moreover, it aids in predicting the effectiveness of the agent upon release and provides insight into the selective forces that are limiting the adaptation of indigenous species to the new pest. We use this knowledge to give future research directions for the development of selective breeding methods for biocontrol agents.

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Laboratory Evaluation of Isaria fumosorosea CCM 8367 and Steinernema feltiae Ustinov against Immature Stages of the Colorado Potato Beetle

Cited by 28 publications

References 63 publications

Efficacy of Topical Application, Leaf Residue or Soil Drench of Blastospores of Isaria fumosorosea for Citrus Root Weevil Management: Laboratory and Greenhouse Investigations

Efficacy of Topical Application, Leaf Residue or Soil Drench of Blastospores of Isaria fumosorosea for Citrus Root Weevil Management: Laboratory and Greenhouse Investigations

Paecilomyces and Its Importance in the Biological Control of Agricultural Pests and Diseases

Optimization of native biocontrol agents, with parasitoids of the invasive pest Drosophila suzukii as an example

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