Laboratory Evaluation of the Cellular Immune System

Riley, Roger S.; Mageau, Ronald; Ben‐Ezra, Jonathan

doi:10.1016/b978-1-4377-0974-2.00045-2

Cited by 2 publications

(1 citation statement)

References 282 publications

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“…In this analysis, microscopy is used to investigate the size, shape, and morphology of individual circulating blood cells [10]. High throughput laser-based single-cell analyses, including flow cytometry, are also common in the clinical laboratory [29][30][31]. Flow cytometry has been used clinically to characterize leukemic cells and immune deficiencies, to predict response to monoclonal antibody therapy, and to screen for genetic diseases [32].…”

Section: Introduction 1lab Medicine and Single-cell Assaysmentioning

confidence: 99%

Preserving single cells in space and time for analytical assays

Gallion

Anttila

Abraham

et al. 2020

TrAC Trends in Analytical Chemistry

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Analytical assays performed within clinical laboratories influence roughly 70% of all medical decisions by facilitating disease detection, diagnosis, and management. Both in clinical and academic research laboratories, single-cell assays permit measurement of cell diversity and identification of rare cells, both of which are important in the understanding of disease pathogenesis. For clinically utility, the single-cell assays must be compatible with the clinical workflow steps of sample collection, sample transportation, pre-analysis processing, and singlecell assay; therefore, it is paramount to preserve cells in a state that resembles that in vivo rather than measuring signaling behaviors initiated in response to stressors such as sample collection and processing. To address these challenges, novel cell fixation (and more broadly, cell preservation) techniques incorporate programmable fixation times, reversible bond formation and cleavage, chemoselective reactions, and improved analyte recovery. These technologies will further the development of individualized, precision therapies for patients to yield improved clinical outcomes.

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Section: Introduction 1lab Medicine and Single-cell Assaysmentioning

confidence: 99%

Preserving single cells in space and time for analytical assays

Gallion

Anttila

Abraham

et al. 2020

TrAC Trends in Analytical Chemistry

View full text Add to dashboard Cite

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Immunológiai eltérések kimutatása laboratóriumi módszerekkel primer immunhiányos betegségekben

Nagy

2018

Orvosi Hetilap

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In primary immunodeficiencies, the malfunction of the immune system is caused by genetic alterations. The physician proposes the most probable diagnosis based on symptoms, clinical signs, the family history and the results of the pathogen identification. To confirm this clinical suspicion, it is essential that the immunological malfunction be tested using in vitro diagnostic procedures. This paper summarizes the screening, confirmatory and disease-specific laboratory methods capable of testing the antibody response, the T cells, the phagocytic function, the complement system and other components of the innate immune system. The genetic tests necessary to make the final diagnosis are beyond the scope of this publication. Orv Hetil. 2018; 159(49): 2087–2094.

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Laboratory Evaluation of the Cellular Immune System

Cited by 2 publications

References 282 publications

Preserving single cells in space and time for analytical assays

Preserving single cells in space and time for analytical assays

Immunológiai eltérések kimutatása laboratóriumi módszerekkel primer immunhiányos betegségekben

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