Laboratory evaluation of the UniCel DxI 800 analyser (Beckman Coulter) for detecting HBV and HCV serological markers

Miédougé, Marcel; Abravanel, Florence; Lalanne, Colette; Sauné, Karine; Izopet, Jacques

doi:10.1016/j.jcv.2008.10.012

Cited by 9 publications

(4 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Kappa coefficient of correlation reached a value of 0.92, much better than seen with other systems. 9 Contrary to reports from other comparisons, there were very few samples with major disagreement in titer and the overall findings of our correlation analysis was fairly homogenous. 10 Notably, no discrepant results were found for patients presenting a vaccine serological profile.…”

Section: Discussioncontrasting

confidence: 99%

Performance of the DiaSorin LIAISON® anti-HBs II for the detection of hepatitis B surface antibodies: Comparison with the Abbott Architect anti-HBs assay

Kinn

Akhavan

Agut

et al. 2011

Journal of Clinical Virology

View full text Add to dashboard Cite

Section: Discussioncontrasting

confidence: 99%

Performance of the DiaSorin LIAISON® anti-HBs II for the detection of hepatitis B surface antibodies: Comparison with the Abbott Architect anti-HBs assay

Kinn

Akhavan

Agut

et al. 2011

Journal of Clinical Virology

View full text Add to dashboard Cite

“…Both ZiehlNeelsen stains and Löwenstein culture of samples from different locations were carried out in order to confirm the presence of extrapulmonary TB in patients with consistent symptomatology. For HBV screening, surface antigen and anti-core IgM and IgG were detected using indirect chemiluminescent techniques, and for HCV diagnosis, antibodies anti-HCV (indirect chemiluminescent technology) were studied and if a confirmatory test was needed, an immunoblot test (RIBA) was then performed [9].…”

Section: Methodsmentioning

confidence: 99%

Infectious Diseases in Immigrant Population Related to the Time of Residence in Spain

Cobo

Salas-Coronas

Cabezas-Fernández

et al. 2014

J Immigrant Minority Health

View full text Add to dashboard Cite

The aim of this study was to evaluate the data on the main imported infectious diseases and public health issues arising from the risk of transmission of tropical and common diseases in the immigrant population. During the period of study, 2,426 immigrants were attended in the Tropical Medicine Unit of the Hospital of Poniente. For each patient, a complete screening for common and tropical diseases was performed. The prevalence and main features of intestinal and urinary parasites, microfilarias, Chagas disease, malaria, hepatitis B (HBV) and C (HCV) viruses, extrapulmonary tuberculosis and syphilis was investigated taking into account the length of stay in Spain. Sub-Saharan Africa patients who had lived for <3 years in Spain had a high significantly number of infections produced by hookworms, Ascaris lumbricoides, Trichuris trichiura, Schistosoma mansoni, Giardia lamblia, Entamoeba histolytica/dispar and Plasmodium spp. In patients who had lived for more than 3 years, there were significantly high rates of HBV infections, although HBV rates in sub-Saharan African patients are high even if the patients have been in Spain for <3 years. However, patients with large stays in Spain had also an important number of parasitological diseases. The main objective of the diagnosis is to avoid important public health problems and further complications in patients. It is advisable to carry out a screening of the main transmissible infections in all immigrant population regardless of the time outside their country. This screening should be individualized according to the geographical area of origin.

show abstract

“…Also, HBV detection methods differ in developed and developing countries according to the level of endemic HBV infections. Besides, automated HBsAg detection immunoassay systems have been developed and widely used [6][7][8][9]. However, high specificity enzyme-linked immunosorbent assays (ELISAs) are still commonly used [4,5,10,11].…”

Section: Article; Medical Biotechnology (Mb)mentioning

confidence: 99%

Cloning of anti-HBsAg single-chain variable fragments from hybridoma cells for one-step ELISA

Erdağ

Balcıoğlu

Bahadır

et al. 2017

Biotechnology & Biotechnological Equipment

View full text Add to dashboard Cite

Hepatitis B virus (HBV) infection is a worldwide health problem. More than 400 million people are chronic HBV carriers in the world. Infected individuals are at a high risk of developing liver cirrhosis and hepatocellular carcinoma as the main consequences of HBV. The discoveries of fast diagnostic systems and new therapeutic applications are crucial in the fight against viral hepatitis. In this paper we present the generation of a single-chain variable fragment (scFv) from a mouse monoclonal antibody specific to the HBV surface antigen (HBsAg) and demonstrate its expression as a bacterial alkaline phosphatase (AP) fusion protein. In this study, we constructed scFvs from hybridoma cells expressing HBsAg-specific antibody using phage display technology and expressed them in Escherichia coli. The anti-HBsAg scFvs were inserted into pQE-2 vector to produce scFv antibody genetically fused to bacterial AP. Reproducibility of the recombinant HBsAg-scFv fusion protein was tested using Enzyme-linked Immunosorbent Assay (ELISA). Present preliminary findings indicate that the anti-HBsAg-scFv AP conjugate could be further used for the development of one-step ELISA for the detection of HBV.

show abstract

Laboratory evaluation of the UniCel DxI 800 analyser (Beckman Coulter) for detecting HBV and HCV serological markers

Cited by 9 publications

References 16 publications

Performance of the DiaSorin LIAISON® anti-HBs II for the detection of hepatitis B surface antibodies: Comparison with the Abbott Architect anti-HBs assay

Performance of the DiaSorin LIAISON® anti-HBs II for the detection of hepatitis B surface antibodies: Comparison with the Abbott Architect anti-HBs assay

Infectious Diseases in Immigrant Population Related to the Time of Residence in Spain

Cloning of anti-HBsAg single-chain variable fragments from hybridoma cells for one-step ELISA

Contact Info

Product

Resources

About