2012
DOI: 10.1186/1475-2859-11-1
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Laboratory evolution of copper tolerant yeast strains

Abstract: BackgroundYeast strains endowed with robustness towards copper and/or enriched in intracellular Cu might find application in biotechnology processes, among others in the production of functional foods. Moreover, they can contribute to the study of human diseases related to impairments of copper metabolism. In this study, we investigated the molecular and physiological factors that confer copper tolerance to strains of baker's yeasts.ResultsWe characterized the effects elicited in natural strains of Candida hum… Show more

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Cited by 202 publications
(143 citation statements)
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“…We show here that although YPD and serum have similar total transition metals, they widely vary in terms of Cu bioavailability, demonstrated by the ability of CP to withhold Cu from serum but not from YPD. Cu availability in YPD medium is particularly low, reflecting strong Cu chelating components of this complex medium (67). Typical laboratory growth media, whether they are chemically defined or more complex (e.g., YPD), cannot readily replicate the metal environment of an animal host, although animal serum is expected to contain many of the natural sources.…”
Section: Discussionmentioning
confidence: 99%
“…We show here that although YPD and serum have similar total transition metals, they widely vary in terms of Cu bioavailability, demonstrated by the ability of CP to withhold Cu from serum but not from YPD. Cu availability in YPD medium is particularly low, reflecting strong Cu chelating components of this complex medium (67). Typical laboratory growth media, whether they are chemically defined or more complex (e.g., YPD), cannot readily replicate the metal environment of an animal host, although animal serum is expected to contain many of the natural sources.…”
Section: Discussionmentioning
confidence: 99%
“…In a previous work, we exploited evolutionary engineering to obtain yeast cells tolerant to the presence of high Cu concentrations (up to 2.5 g l 21 ) in the growth medium (Adamo et al, 2012). Here, we report that in one evolved S. cerevisiae strain, increased CUP1 copy number is the more marked event associated with enhanced Cu tolerance.…”
Section: Introductionmentioning
confidence: 99%
“…S. cerevisiae cells used in this work derive from a natural Cu-sensitive strain -the BL7 strain described in a previous work (Adamo et al, 2012) -whose growth is inhibited at 1.0 g CuSO 4 l 21 in the culture medium. This parental strain is referred to in the text as non-evolved.…”
Section: Methodsmentioning
confidence: 99%
“…Therefore, non-GMO strategies, based on random mutagenesis and/or directed evolution, are often more appropriate to improve complex industrially relevant yeast traits (reviewed in reference 9). These approaches were already successfully applied to improve several stress-related phenotypes of industrial yeast strains, including ethanol tolerance (10), acetic acid tolerance (11), and copper resistance (12). Perhaps the most important factor determining the success of these strategies is the availability of an easy way to identify the few superior cells among a large pool of inferior variants.…”
mentioning
confidence: 99%