Laboratory Handbook for the Fractionation of Natural Extracts

Houghton, Peter J.; Raman, Amala

doi:10.1007/978-1-4615-5809-5

Cited by 322 publications

(300 citation statements)

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“…Standard methods were used to screen the phytochemicals such as alkaloids, saponins, phenols, flavonoids and tannins present in Berberis holstii extracts [16,17].…”

Section: Phytochemical Determinationmentioning

confidence: 99%

Antidiabetic Activity of Administration of Aqueous Extract of Berberis holstii

Kimani¹,

Njangiru²,

Njagi³

et al. 2017

J Diabetes Metab

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Diabetes mellitus in Kenya is of public health concern since it causes significant mortality and complications that are long term. Though conventional drugs are used in the management of diabetes mellitus they are expensive, unavailable and also have numerous side effects. Berberis are medicinally important and are used for various purposes. Berberis holstii has traditionally been used in the management of diabetes; however, the efficacy of this herb needs to be evaluated scientifically.The study aimed at determining the in vivo antidiabetic effects of root extracts of Berberis holstii in alloxaninduced diabetic male mice. In this study, eight groups of animals with five mice each were used. The reductions in blood glucose relative to their initial values were determined after oral and intraperitoneal administration of 25, 48.4, 93.5, 180.9, and 350 mg/kg body weight. Standard hypoglycemic used to compare results were 200 mg/kg body weight (4.6 mg of glibenclamide) for oral route and 1 IU/kg body weight dose of insulin for an intraperitoneal route. The levels of glucose were measured at the start of the experiment and repeated at intervals of 2 hours up to 10 hours and at 24 hours after administering the drug. Significant (ρ<0.05) reduction in blood sugar relative to their initial values was determined for all treated diabetic groups at the end of the experiment. The composition of phytochemicals was determined using standard procedures. The results were analyzed using ANOVA and post-ANOVA.The results showed that the plant extract at 25, 48.4, 93.5, 180.9, and 350 mg/kg body weight administered effectively reduced blood glucose levels (up to 30% reduction) which could be associated with the phytochemicals present which possesses antidiabetic activities. The extracts contained tannins, flavonoids, saponins, phenols and alkaloids. The study, therefore, recommends the use of Berberis holstii in the management of diabetes mellitus.

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“…Standard methods were used to screen the phytochemicals such as alkaloids, saponins, phenols, flavonoids and tannins present in Berberis holstii extracts [16,17].…”

Section: Phytochemical Determinationmentioning

confidence: 99%

Antidiabetic Activity of Administration of Aqueous Extract of Berberis holstii

Kimani¹,

Njangiru²,

Njagi³

et al. 2017

J Diabetes Metab

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“…Qualitative phytochemical screening of Lippia multiflora was carried out on the powdery samples, after extraction with aqueous solvent, using the standardly employed precipitation and coloration reactions as described by Houghton and Raman (1998) and used by Adjatin et al (2013), Assogba et al (2015) and Nounagnon et al (2016) …”

Section: Phytochemical Analysismentioning

confidence: 99%

Phytochemical Screening and Toxicity of Lippia multiflora Moldenke, a Minor Aromatic Leafy Vegetable Consumed in Benin

Djengue¹,

Dansi²,

Assogba³

et al. 2017

Int. J. Curr. Res. Biosci. Plant Biol.

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A b s t r a c t A r t i c l e I n f oLippia multiflora Moldenke, is a minor aromatic leafy vegetable mainly consumed in central Benin that belong to the country's neglected and underutilised species (NUS). Extracts of its leaves and flowers collected from six different zones (Bantè, Dassa-Zoumè, Djidja, Glazoué, Savalou and Savè) were assessed for phytochemical composition and tested for cytotoxicity using brine shrimp lethality bioassay. The phytochemical screening revealed the presence of almost the same phytochemical groups (cathetic and gallic tannins, flavonoids, anthocyanins, leucoanthocyanes, triterpenoids, mucilage, coumarins and the reducing compounds) in both leaves and flowers from all sites with variable abundances. Alkaloids were found only in leaves while steroids, quinone derivatives and combined anthracene C-heterosides were identified uniquely in flowers. The nature and the functions of the compounds found justify the perceived medicinal values of the species. Extracts from both leaves and flowers showed positive action on shrimp larvae but with CL 50 of (13.28±1.52 mg/ml for leaves; 0.46±0.05 mg/ml for flowers) superior to 0.1mg/ml, hence indicating the non-toxicity of Lippia multiflora.

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“…The yield of powder was 48% from water extracts, 34% from acetone and 21% from ethanol extracts for the root while 51%, 32% and 19% (w/w) were obtained for the stem bark, respectively. Farnsworth (1966), Eaton (1989), Silva et al (1993), Harborne (1998) and Houghton and Raman (1998).…”

Section: Preparation Of Extractsmentioning

confidence: 99%

“…The chloroform layer separated and concentrated to about 2 mL and 2-3 mL ammonia solution added. The bonded aglycone was tested using modified Börntrager's test Houghton and Raman (1998).…”

Section: Source Of Test Organismsmentioning

confidence: 99%

Antimicrobial Potency of Hydro, acetone and Ethanolic extracts of <i>Treculia africana</i> Decne Root and Stem Bark

Oyeyipo¹,

Onasoga²

2016

Journal of Applied Sciences and Environmental Management

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ABSTRACT:Treculia africana is a plant that is used in traditional medicine for the treatment of diverse ailments. To investigate the scientific basis for the use of the plant, the antimicrobial activities/potency of the root and stem bark extracts were evaluated against common gram negative and gram positive bacteria as well as yeast. The chemical constituents of the plant in addition to the effect of temperature and pH on its antimicrobial potencies were investigated. The phytochemical constituents of the dried powdered plant parts were extracted using aqueous and organic solvents (acetone and ethanol). The antimicrobial activity of the concentrated extracts was evaluated by determining the diameter of zone of inhibition against both gram negative and gram positive bacteria and yeast, using the disc diffusion technique. Phytochemical studies revealed the presence of glycosides, anthraquinone, free aglycone, saponin, steroids and polyphenols. The extracts were active against both gram positive and gram negative bacteria. The activities of the plant extracts were not affected at varied temperatures (4 o C, 30 o C, 60 o C and 100 o C), but decreased at alkaline pH. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the extracts on the test organisms revealed lowest MIC and MBC against Salmonella paratyphi, Salmonella typhi and Bacillus subtilis with highest MIC and MBC exhibited against Staphylococcus aureus. Generally, there was significant difference (p<0.05) in the antimicrobial activities of the test extracts and control antibiotics, with the control antibiotics exerting broader antimicrobial activity. Treculia africana demonstrated broad spectrum antibacterial activity and a potential source of new classes of antibiotics that could be useful for infectious disease chemotherapy and control. ©JASEM

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Laboratory Handbook for the Fractionation of Natural Extracts

Cited by 322 publications

References 0 publications

Antidiabetic Activity of Administration of Aqueous Extract of Berberis holstii

Antidiabetic Activity of Administration of Aqueous Extract of Berberis holstii

Phytochemical Screening and Toxicity of Lippia multiflora Moldenke, a Minor Aromatic Leafy Vegetable Consumed in Benin

Antimicrobial Potency of Hydro, acetone and Ethanolic extracts of <i>Treculia africana</i> Decne Root and Stem Bark

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