Laboratory metabolic evolution improves acetate tolerance and growth on acetate of ethanologenic Escherichia coli under non-aerated conditions in glucose-mineral medium

Fernández‐Sandoval, Marco T.; Huerta-Beristain, Gerardo; Trujillo-Martínez, Berenice; Bustos, Patricia; González, Víctor; Bolívar, Francisco; Gosset, Guillermo; Martı́nez, Alfredo

doi:10.1007/s00253-012-4177-y

Cited by 66 publications

(71 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Classical evolution methods involving strain enrichment under selective pressure have been used with the goal of improving acetate tolerance in order to improve acetate production by Clostridium thermoaceticum (Reed et al 1987) and A. aceti (Steiner and Sauer 2003a). This technique has also been used to increase acetate tolerance of Zymomonas mobilis (Joachimsthal et al 1998) and E. coli (Steiner and Sauer 2003b;Fernández-Sandoval et al 2012). Improving acetate tolerance in E. coli has also been addressed through the use of global transcription machinery engineering (gTME) (Chong et al 2013) and scalar analysis of library enrichments (SCALES) (Sandoval et al 2011).…”

Section: Other Bacteriamentioning

confidence: 98%

“…It is a hindrance due to its presence at inhibitory concentrations in biomass hydrolysate (Martinez et al 2001;Mills et al 2009;Taylor et al 2012), though at low concentrations, acetate has been observed to slightly increase product titers (Fernández-Sandoval et al 2012). It is used as a substrate in the production of polyhydroxyalkanoates, such as poly-3-hydroxybutyrate (PHB) (Wang and Yu 2000).…”

Section: Biorenewablesmentioning

confidence: 98%

See 1 more Smart Citation

Adaptation and tolerance of bacteria against acetic acid

Trček

Mira

Jarboe

2015

Appl Microbiol Biotechnol

195

100

View full text Add to dashboard Cite

Acetic acid is a weak organic acid exerting a toxic effect to most microorganisms at concentrations as low as 0.5 wt%. This toxic effect results mostly from acetic acid dissociation inside microbial cells, causing a decrease of intracellular pH and metabolic disturbance by the anion, among other deleterious effects. These microbial inhibition mechanisms enable acetic acid to be used as a preservative, although its usefulness is limited by the emergence of highly tolerant spoilage strains. Several biotechnological processes are also inhibited by the accumulation of acetic acid in the growth medium including production of bioethanol from lignocellulosics, wine making, and microbe-based production of acetic acid itself. To design better preservation strategies based on acetic acid and to improve the robustness of industrial biotechnological processes limited by this acid's toxicity, it is essential to deepen the understanding of the underlying toxicity mechanisms. In this sense, adaptive responses that improve tolerance to acetic acid have been well studied in Escherichia coli and Saccharomyces cerevisiae. Strains highly tolerant to acetic acid, either isolated from natural environments or specifically engineered for this effect, represent a unique reservoir of information that could increase our understanding of acetic acid tolerance and contribute to the design of additional tolerance mechanisms. In this article, the mechanisms underlying the acetic acid tolerance exhibited by several bacterial strains are reviewed, with emphasis on the knowledge gathered in acetic acid bacteria and E. coli. A comparison of how these bacterial adaptive responses to acetic acid stress fit to those described in the yeast Saccharomyces cerevisiae is also performed. A systematic comparison of the similarities and dissimilarities of the ways by which different microbial systems surpass the deleterious effects of acetic acid toxicity has not been performed so far, although such exchange of knowledge can open the door to the design of novel approaches aiming the development of acetic acid-tolerant strains with increased industrial robustness in a synthetic biology perspective.

show abstract

Section: Other Bacteriamentioning

confidence: 98%

Section: Biorenewablesmentioning

confidence: 98%

Adaptation and tolerance of bacteria against acetic acid

Trček

Mira

Jarboe

2015

Appl Microbiol Biotechnol

195

100

View full text Add to dashboard Cite

show abstract

“…Escherichia coli has been shown to be an efficient fermentation strain for mixed hexose-pentose solutions (Fernández-Sandoval et al 2012). In this work, sulfuric acid prehydrolysates of OTB were submitted to fermentability tests by an ethanologenic E. coli.…”

Section: Fermentability Tests Of Prehydrolysatesmentioning

confidence: 99%

Design and Optimization of Sulfuric Acid Pretreatment of Extracted Olive Tree Biomass Using Response Surface Methodology

et al. 2017

View full text Add to dashboard Cite

Olive tree biomass (OTB) represents an interesting feedstock for bioethanol production. In this study, olive tree pruning was water extracted and pretreated by dilute sulfuric acid to achieve high sugar recoveries from cellulosic and hemicellulosic fractions. Temperature (160 to 200 °C), acid concentration (0 to 8 g acid/100 g extracted raw material), and solids loading (15% to 35% w/v) were selected as operation variables and modified according to a Box-Behnken experimental design. The optimal conditions for the acid pretreatment were 160 °C, 4.9 g sulfuric acid/100 g biomass, and 35% solids loading (w/v), according to multiple criteria that considered the maximization of both the hemicellulosic sugars concentration in prehydrolysate and the overall sugar yield. These optimized conditions yielded a sugar concentration of 79.8 g/L, corresponding to an overall yield of 39.8 g total sugars/100 g extracted OTB. The fermentability of hemicellulosic sugars prehydrolysates from the acid pretreatment was evaluated by Escherichia coli after a detoxification stage by overliming. The prehydrolysates with lower concentrations of toxic compounds were fermented and achieved ethanol yields higher than 80% of the theoretical ethanol yield.

show abstract

“…The objective of this work was to produce ethanol fuel from hemicellulosic sugars of rape straw using an ethanologenic E. coli that is a microorganism able to produce ethanol from xylose and glucose in presence of high acetic acid concentrations (Fernández-Sandoval et al, 2012). For the first time with this feedstock, bioethanol production from hemicellulose sugars has been reported.…”

Section: Acid Pretreatmentmentioning

confidence: 99%

“…Escherichia coli is a bacterium able to carry out the co-fermentation of C5 and C6 sugars although without high ethanol production. However, several researches have been carried out engineering of bacterial pathways to generate E. coli strains able to achieve higher ethanol yields (Fernández-Sandoval et al, 2012). Different genetically engineered E. coli strains have been used to ferment hydrolysates from corn stover (Jin et al, 2012), Eucalyptus (Castro et al, 2014), sugarcane bagasse (Geddes et al, 2011) or wheat straw (Saha et al, 2011) yielding higher ethanol productions and showing more resistance to toxic compounds than traditional ethanologenic microorganisms.…”

Section: Acid Pretreatmentmentioning

confidence: 99%

Hemicellulose-derived sugars solubilisation of rape straw. Cofermentation of pentoses and hexoses by Escherichia coli

López-Linares

Cara-Corpas

Ruiz

et al. 2015

Span. j. agric. res.

View full text Add to dashboard Cite

Bioconversion of hemicellulose sugars is essential for increasing fuel ethanol yields from lignocellulosic biomass. We report for the first time with rape straw, bioethanol production from hemicellulose sugars. Rape straw was pretreated at mild conditions with sulfuric acid to solubilize the hemicellulose fraction. This pretreatment allows obtaining a prehydrolysate, consisting basically in a solution of monomeric hemicellulosic sugars, with low inhibitor concentrations. The remaining water insoluble solid constitutes a cellulose-enriched, free of extractives material. The influence of temperature (120ºC and 130ºC), acid concentration (2-4% w/v) and pretreatment time (30-180 min) on hemicellulose-derived sugars solubilisation was evaluated. The highest hemicellulosic sugars recovery, 72.3%, was achieved at 130ºC with 2% sulfuric acid and 60 min. At these conditions, a concentrated sugars solution, 52.4 g/L, was obtained after three acid consecutive contacts, with 67% xylose and acetic acid concentration above 4.5 g/L. After a detoxification step by activated charcoal or ion-exchange resin, prehydrolysate was fermented by ethanologenic Escherichia coli. An alcoholic solution of 25 g/L and 86% of theoretical ethanol yield was attained after 144 h when the prehydrolysate was detoxified by ion-exchange resin. The results obtained in the present work show sulfuric acid pretreatment under mild conditions and E. coli as an interesting process to exploit hemicellulosic sugars in rape straw.Additional key words: agricultural residue; acid pretreatment; xylose; E. coli; bioethanol production

show abstract

Laboratory metabolic evolution improves acetate tolerance and growth on acetate of ethanologenic Escherichia coli under non-aerated conditions in glucose-mineral medium

Cited by 66 publications

References 32 publications

Adaptation and tolerance of bacteria against acetic acid

Adaptation and tolerance of bacteria against acetic acid

Design and Optimization of Sulfuric Acid Pretreatment of Extracted Olive Tree Biomass Using Response Surface Methodology

Hemicellulose-derived sugars solubilisation of rape straw. Cofermentation of pentoses and hexoses by Escherichia coli

Contact Info

Product

Resources

About