Laboratory procedures for the detection and identification of cutaneous non‐tuberculous mycobacterial infections

Nakanaga, Kazue; Hoshino, Yoshihiko; Yotsu, Rie Roselyne; Makino, Masahiko; Ishii, Norihisa

doi:10.1111/1346-8138.12047

Cited by 20 publications

(11 citation statements)

References 55 publications

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“…7 Notably, a visible colony of M. chelonae was identified after 9-week incubation in the present case. This might be explained by the fact that the optimal temperature for M. chelonae growth is < 30°C, 8 whereas the biopsy samples were cultured using Ogawa medium and the BD Bactec MGIT 960 system utilizing a stable temperature of 35-37°C.…”

Section: Repeated Skin Sampling and Prolonged Incubation Period Identsupporting

confidence: 45%

Repeated skin sampling and prolonged incubation period identified cutaneous M ycobacterium chelonae infection on the face in an immunocompetent man

et al. 2014

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Section: Repeated Skin Sampling and Prolonged Incubation Period Identsupporting

confidence: 45%

Repeated skin sampling and prolonged incubation period identified cutaneous M ycobacterium chelonae infection on the face in an immunocompetent man

et al. 2014

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“…Bacterial DNA was extracted from the skin biopsy specimens and culture fluid, and it was amplified through polymerase chain reaction (PCR) using primer sets that targeted the 16S rRNA gene, rpoB, hsp65 and internal transcribed spacer (ITS) region, as previously described. 4 Amplified PCR products were sequenced on an ABI Prism 3100 PCR Genetic Analyzer (Applied Biosystems, Foster City, CA, USA). The 16S rRNA gene, rpoB, hsp65 and ITS region sequences showed a 100%, 100%, 99.66% and 99.7% match, respectively, with the corresponding regions of M. haemophilum, but it harbored novel gene sequences.…”

Section: Case Reportmentioning

confidence: 99%

Case of Mycobacterium haemophilum misdiagnosed as Mycobacterium intracellulare due to one base insertion in the bacterial genome

Nishikawa

Yamada

Kanki

et al. 2017

The Journal of Dermatology

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Mycobacterium haemophilum is a slow-growing, non-tuberculous mycobacteria that causes cutaneous infection. We describe a case of cutaneous infection in a 68-year-old Japanese man with polymyositis. This was caused by M. haemophilum harboring one base insertion in gene sequence. At first, the causal microorganism was misidentified as M. intracellulare by COBAS â TaqMan â MAI test. However, poor growth on Ogawa media and growth enhancement on 7H11C agar around a hemin-containing disk prompted us to reinvestigate the causal microorganisms, which were revealed to be M. haemophilum. Amplified polymerase chain reaction products were sequenced, and the 16S rRNA gene, rpoB, hsp65 and internal transcribed spacer region sequences showed a 100%, 100%, 99.66% and 99.7% match, respectively, with the corresponding regions of M. haemophilum, but it harbored a novel gene sequence in hsp65. The sequences determined by gene analysis of the M. haemophilum strain were deposited into the International Nucleotide Sequence Database. Although numerous cases of M. haemophilum infection have been reported in other countries, only six cases have been reported in Japan to date. It could be possible that this novel mutation lead to misdiagnosis. As M. haemophilum prefers a lower growth temperature (30-32°C) and it requires iron in the culture medium, M. haemophilum could be misidentified or overlooked. Accordingly, a M. haemophilum infection should be considered in cases of cutaneous infection of the body sites, of which surface temperature is low.

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“…The isolation of AM is usually based on advanced tools such as tissue culture and PCR. The new analytical procedures including novel culture methods (such as culture on Ogawa medium or Middlebrook 7H10/7H11 agar medium) and novel commercial assay based on the nucleic acid sequence‐based amplification, DNA–DNA hybridization assays, DNA sequences of heat shock protein 65, rpoB and the 16S–23S internal transcribed spacer region, and DNA strip techniques, allowing the 16S rRNA amplification‐based detection of AM infection have led to significant advancements in understanding the origin and progression of AM infections . On the other hand, the role of direct smear is usually limited, while skin biopsy is concerned mainly in the demonstration of granulomatous reaction.…”

Section: Discussionmentioning

confidence: 99%

Atypical mycobacterial cutaneous infections in Egyptians: A clinicopathological study

El‐Khalawany

2014

The Journal of Dermatology

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Atypical mycobacteria comprise an uncommon heterogenous non-tuberculous group of acid-fast bacteria that rarely involve skin. The pattern of atypical mycobacterial cutaneous infections (AMCI) has not been previously studied in Egypt. The aim of this study was to describe the clinical characteristics, pathological features and species profile of AMCI among Egyptian patients. A retrospective study included 46 cases, diagnosed with AMCI during the period 2002 to 2012. The study included 34 males (73.9%) and 12 females (26.9%). The average age of patients was 39 years while the average duration of lesions was 15 months. The lesions were mostly located on the extremities (91.3%) and there was predominance of single (65.2%) and nodular (41.4%) lesions. History of trauma was confirmed in 91.3%. Histologically, the granulomas were mostly superficial (67.4%) with predominance of nodular suppurative pattern (84.8%). Other significant histological findings included epidermal hypertrophy (100%), presence of large-sized multinucleated giant cells (87%) and intrafollicular neutrophilic abscesses (84.8%). The diagnosis was proved by direct smear in 6.5%, skin biopsy in 10.9%, tissue culture in 47.8% and polymerase chain reaction (PCR) in 34.8%. Isolated species included Mycobacterium marinum (84.8%), Mycobacterium fortuitum (10.9%) and Mycobacterium kansasii (4.3%). Although the results of this study recommend that the diagnosis of AMCI is based mainly on culture and PCR, other clinicopathological features such as history of trauma, acral location of the lesion and suppurative granulomatous reaction with intrafollicular abscesses could be helpful clues in suspecting AMCI.

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Laboratory procedures for the detection and identification of cutaneous non‐tuberculous mycobacterial infections

Cited by 20 publications

References 55 publications

Repeated skin sampling and prolonged incubation period identified cutaneous M ycobacterium chelonae infection on the face in an immunocompetent man

Repeated skin sampling and prolonged incubation period identified cutaneous M ycobacterium chelonae infection on the face in an immunocompetent man

Case of Mycobacterium haemophilum misdiagnosed as Mycobacterium intracellulare due to one base insertion in the bacterial genome

Atypical mycobacterial cutaneous infections in Egyptians: A clinicopathological study

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