Laboratory Soft X-Ray Microscopy with an Integrated Visible-Light Microscope—Correlative Workflow for Faster 3D Cell Imaging

Dehlinger, A.; Seim, Christian; Stiel, H.; Twamley, Shailey; Ludwig, Antje; Kördel, Mikael; Grötzsch, Daniel; Rehbein, Stefan; Kanngießer, Birgit

doi:10.1017/s1431927620024447

Cited by 10 publications

(4 citation statements)

References 20 publications

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“…XRF projection images were extracted from the XRF detectors by selecting a spectral range corresponding to the elements of interest, [17.1, 17.7] keV for Mo and [18.9, 19.6] keV for Ru. The current setup could achieve a spatial resolution of 200 µm [12]. MoO2 NPs and Ru NPs exhibited a distinct biodistribution (Figure 6b).…”

Section: In Vivo X-ray Fluorescence Imagingmentioning

confidence: 99%

“…SXM provides a powerful tool for cellular biology with high resolution and high contrast under unperturbed conditions [4][5][6][7]. The unique properties of soft X-rays, such as the short wavelengths [8,9], allow nanometer-scale resolution of thick samples (>1 µm), making them an attractive alternative to other microscopy techniques for exploring the cell's interior in 2D or 3D imaging [10][11][12][13]. Additionally, SXM eliminates the need for chemical fixation, sectioning, heavy-metal staining, and optical fluorescence labeling [4,[14][15][16].…”

Section: Introductionmentioning

confidence: 99%

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Laboratory Liquid-Jet X-ray Microscopy and X-ray Fluorescence Imaging for Biomedical Applications

Arsana,

Saladino,

Brodin

et al. 2024

IJMS

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Diffraction-limited resolution and low penetration depth are fundamental constraints in optical microscopy and in vivo imaging. Recently, liquid-jet X-ray technology has enabled the generation of X-rays with high-power intensities in laboratory settings. By allowing the observation of cellular processes in their natural state, liquid-jet soft X-ray microscopy (SXM) can provide morphological information on living cells without staining. Furthermore, X-ray fluorescence imaging (XFI) permits the tracking of contrast agents in vivo with high elemental specificity, going beyond attenuation contrast. In this study, we established a methodology to investigate nanoparticle (NP) interactions in vitro and in vivo, solely based on X-ray imaging. We employed soft (0.5 keV) and hard (24 keV) X-rays for cellular studies and preclinical evaluations, respectively. Our results demonstrated the possibility of localizing NPs in the intracellular environment via SXM and evaluating their biodistribution with in vivo multiplexed XFI. We envisage that laboratory liquid-jet X-ray technology will significantly contribute to advancing our understanding of biological systems in the field of nanomedical research.

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Section: In Vivo X-ray Fluorescence Imagingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Laboratory Liquid-Jet X-ray Microscopy and X-ray Fluorescence Imaging for Biomedical Applications

Arsana,

Saladino,

Brodin

et al. 2024

IJMS

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“…Soft X-ray microscopy is one of the most important nanoprobe techniques (Chu et al, 2008;Kirz, 1974;Spector, 1997;Selin et al, 2015), being widely applied in the development of, but not limited, biological science (Kapishnikov et al, 2017;Dehlinger et al, 2020), material science (Le et al, 2020) and nanotechnology (de Smit et al, 2008). Especially in the 2.3-4.4 nm wavelength range (the 'water window'), it is particularly adequate for high-resolution imaging of biological specimens (Kepsutlu et al, 2020;Chiappi et al, 2016) because of the high optical contrast of the X-rays in this energy range between carbon and water present in testing materials.…”

Section: Introductionmentioning

confidence: 99%

High-efficiency focusing and imaging by dielectric kinoform zone plate lenses with soft X-rays

Tong¹,

Chen²,

Xu³

et al. 2023

J Synchrotron Rad

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With fast advances in enhancing the focusing/imaging resolution of Fresnel zone plate lenses toward sub-10 nm, low diffraction efficiency in connection with their rectangular zone shape still remains a big issue in both soft and hard X-ray microscopy. In hard X-ray optics, encouraging progress has recently been reported in our earlier attempts of high focusing efficiency by 3D kinoform shaped metallic zone plates, formed by greyscale electron beam lithography. This paper addresses our efforts towards high focusing/imaging efficiency by developing a novel dielectric kinoform zone plate lens for soft X-rays. The effects of the zone materials and zone shapes on the focusing/imaging quality were first theoretically investigated by a modified thin-grating-approximation method, revealing superior efficiencies of dielectric kinoform zone plates over rectangular ones in metals. Optical characterizations of replicated dielectric kinoform zone plates by greyscale electron beam lithography demonstrate a focusing efficiency of 15.5% with a resolution of 110 nm in the water window of X-rays. Apart from high efficiency, the novel kinoform zone plate lenses developed in this work exhibit significant advantages over conventional zone plates, i.e. simplified process, low cost and no need for a beamstop.

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“…Extensive applications of x-ray microscopy in bio-science [1][2][3][4][5][6][7] demand fast advances of soft x-ray lenses (0.2-2 keV) such as Fresnel zone plates as critical components in x-ray nanoprobes toward high resolution and high efficiency. In the past decades, high resolution zone plates have been frequently reported [8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

A compound Kinoform/Fresnel zone plate lens with 15 nm resolution and high efficiency in soft x-ray

Tong

Chen

et al. 2023

Nanotechnology

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X-ray microscope as an important nanoprobing tool plays a prevailing role in nano-inspections of materials. Despite the fast advances of high resolution focusing/imaging reported, the efficiency of existing high-resolution zone plates is mostly around 5% in soft X-ray and rapidly goes down to 1-2% when the resolution approaches 10 nm. It is well known that the rectangular zone shape, beamstop, limited height/width ratios, material absorption of light and structural defects are likely responsible for the limited efficiency. Although zone plates with Kinoform profile are supposed to be efficient, progress for achieving both high resolution (<30 nm) and high efficiency (>5%) have hardly been addressed in soft X-ray. In this work, we propose a compound Kinoform/Fresnel zone plate (CKZP) by combing a dielectric Kinoform zone plate with a 15 nm resolution zone plate. Greyscale electron beam lithography was applied to form the 3D Kinoform zone plate and atomic layer deposition was carried out to form the binary zone plate. Optical characterizations demonstrated 15-nm resolution focusing/imaging with over 7.8% efficiency in soft X-ray. The origin of the efficiency improvement behind the proposed compound lens is theoretically analyzed and discussed.

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Laboratory Soft X-Ray Microscopy with an Integrated Visible-Light Microscope—Correlative Workflow for Faster 3D Cell Imaging

Abstract: Abstract

Cited by 10 publications

References 20 publications

Laboratory Liquid-Jet X-ray Microscopy and X-ray Fluorescence Imaging for Biomedical Applications

Laboratory Liquid-Jet X-ray Microscopy and X-ray Fluorescence Imaging for Biomedical Applications

High-efficiency focusing and imaging by dielectric kinoform zone plate lenses with soft X-rays

A compound Kinoform/Fresnel zone plate lens with 15 nm resolution and high efficiency in soft x-ray

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