Laboratory surveillance of Salmonella enterica from human clinical cases in Colombia 2005–2011

Rodríguez, Ernesto Suárez; Díaz-Guevara, Paula; Moreno, Jaime; Bautista, Adriana; Montaño, Lucy Angeline; Realpe, María Elena; Gaspera, A. Della; Wiesner, Magdalena

doi:10.1016/j.eimce.2017.06.006

Cited by 17 publications

(18 citation statements)

References 32 publications

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“…In the present study, we described 38 Salmonella isolates (all of them NTS) obtained from urine cultures of patients from seven different hospitals, five from Porto Alegre (the capital city) and two from two other important and populous cities of the Rio Grande do Sul state (Novo Hamburgo and Santa Maria). NTS was isolated from both sexes and all age groups with predominance in adults over 45 years of age, as observed in other reports [16,17,19,21].…”

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confidence: 85%

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Salmonella isolates from urine cultures: serotypes and antimicrobial resistance in hospital settings

et al. 2019

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This study investigated the serotypes and antimicrobial resistance of Salmonella isolates in urine cultures from 38 hospitalized patients. Nine serotypes were detected, and a large proportion was Typhimurium and Enteritidis. The strains presented resistance to 11 different antibiotics. Thirteen isolates (11 from serotype Typhimurium) exhibited multidrug resistance. Keywords Nontyphoid Salmonella. Urinary tract infections. Multidrug resistance Salmonella is an important pathogen causing approximately 550 million infections in the world per year [1]. Typhoid Salmonella (TS) serotypes (Typhi, Paratyphi A and B) are adapted to humans and cause typhoid fever, while nontyphoid Salmonella (NTS) serotypes infect a wide range of hosts, causing symptoms ranging from mild gastroenteritis to severe systemic infection. NTS has increasingly impacted human health, and it is responsible for approximately 93.8 million infections with 155,000 deaths per year [1, 2]. The clinical symptoms include diarrhea, fever, headache, and abdominal pain [3]. Infections by invasive NTS (iNTS) strains can also progress to severe life-threatening sepsis, a common cause of bacteremia in children and adults. The diversity in the clinical manifestations is explained by several factors, highlighting the occurrence of a large range of virulence factors in the bacterial strains [3, 4]. Urinary tract infections (UTIs) by Salmonella are infrequent in routine hospital practice and rarely reported in the scientific literature, although they may cause serious morbidity in immunocompromised and even in immunocompetent patients [5-8]. The present study describes the serotype identification and antimicrobial resistance of Salmonella isolates from urine cultures of hospitalized patients. A total of 38 Salmonella isolates were obtained from urine cultures in different hospitalized patients in Rio Grande do Sul state and sent to a public health laboratory (Laboratório Central do Rio Grande do Sul, LACEN-RS) from 2010 to 2015. Demographic data (such as hospital, city, and date of isolation; age and sex of the patients) were obtained in the clinical forms. The isolates were plated on Salmonella/ Shigella solid media, and presumptive Salmonella colonies were cultured in Triple Sugar Iron (TSI), Urea, Citrate, and Lysine Iron Agar (LIA) slants (Probac, São Paulo, Brazil). The isolates were submitted to aerobic incubation at 37°C for 24 h. Suspected Salmonella colonies were partially tested by agglutination with the main polyvalent O and H antisera (Probac, São Paulo, Brazil). Complete antigenic characterization was carried out for serotype identification in the National Reference Laboratory for Cholera and Enteric Diseases in the Institute Oswaldo Cruz (FIOCRUZ, Rio de Janeiro, RJ, Brazil) by the Kauffmann-White-Le Minor scheme [9]. All isolates were also submitted to DNA extraction and real-time polymerase chain reaction (PCR) targeting the invA

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confidence: 85%

“…The invasiveness characteristics of this serotype also facilitate bacteremia and the occurrence of UTIs [3,4]. Other studies have also shown that this serotype is etiologically associated with UTIs in South America [21,22].…”

mentioning

confidence: 99%

Salmonella isolates from urine cultures: serotypes and antimicrobial resistance in hospital settings

et al. 2019

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“…4,6 Panama was also the major serovar in humans in Colombia and Chile. 9,10 This high prevalence is in contrast to that in other regions of the world, including mainland France (0.5% in 2011), 11 highlighting the specific epidemiology of these regions. In industrialized countries, NTS is transmitted predominantly through commercially produced food contaminated with animal feces.…”

Section: Discussionmentioning

confidence: 83%

Salmonella enterica serovars Panama and Arechavaleta: Risk Factors for Invasive Non-Typhoidal Salmonella Disease in Guadeloupe, French West Indies

Guyomard-Rabenirina

Muanza²,

Bastian

et al. 2018

The American Journal of Tropical Medicine and Hygiene

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A retrospective study was conducted to identify the risk factors associated with bacteremia in infants and children in Guadeloupe, French West Indies. The 171 patients with infection seen between 2010 and 2014 included 155 (90.6%) with acute gastroenteritis, of whom 42 (27.1%) had concomitant bacteremia, and 16 (9.4%) with primary bacteremia. Most cases (97.7%) were in infants and children with no underlying health condition. Two subspecies were recovered: ( = 161, 94.2%) and ( = 10, 5.8%). All but one (serovar Typhi) were non-typhoidal . The most common serovars were Panama ( = 57, 33.3% of isolates) and Arechavaleta ( = 28, 16.4%). Univariate analysis showed a strong association only between age > 6 months and infection with the Panama or Arechavaleta serovar ( = 0.002). The rate of resistance to all classes of antibiotics during the study period was low (< 15%); however, the detection of one extended-spectrum beta-lactamase-producing strain highlights the need for continued monitoring of antimicrobial drug susceptibility. Infection with Panama ( < 0.001) or Arechavaleta ( < 0.001) serovar was significantly associated with bacteremia in a multivariate analysis. These serovars are probably poorly adapted to humans or are more virulent. A delay between onset of symptoms and hospital admission > 5 days ( = 0.01), vomiting ( = 0.001), and increased respiratory rate ( = 0.001) contributed independently to bacteremia in the multivariate analysis. Thus, if non-typhoidal infection is suspected, blood should be cultured and antibiotic treatment initiated in all patients who meet these criteria.

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“…Also, it has been one of the prominent serovars in human clinical cases in Columbia (Rodriguez et al . ).…”

Section: Discussionmentioning

confidence: 97%

Serovars and antimicrobial resistance of non‐typhoidal Salmonella isolated from non‐diarrhoeic dogs in Grenada, West Indies

Amadi

Hariharan

Arya

et al. 2017

Veterinary Medicine & Sci

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Non‐typhoidal salmonellosis remains an important public health problem worldwide. Dogs may harbour Salmonella in their intestines and can easily shed Salmonella in the environment with the possibility of transmission to humans. Thus, monitoring is essential to understand the role of dogs in zoonotic transmission. The objectives of this study were to determine the shedding of Salmonella by owned, apparently healthy dogs in Grenada, West Indies, to identify the serovars, and to examine their antimicrobial susceptibility profiles. Faecal samples collected during August to October, 2016 from 144 non‐diarrhoeic owned dogs were examined by enrichment and selective culture for the presence of Salmonella spp. Eight (5.6%) of the tested animals were culture positive, yielding 35 Salmonella isolates that belonged to six serovars of Salmonella enterica subspecies enterica. These were serovars Arechavaleta from two dogs, Arechavaleta and Montevideo from one dog, and Javiana, Rubislaw, Braenderup and Kiambu from one dog each. All these serovars have been reported as causes of human salmonellosis globally. Antimicrobial susceptibility tests on 35 isolates showed absence of resistance to the currently used drugs for cases of human salmonellosis, including ciprofloxacin and cefotaxime. One isolate (2.9%) was resistant to neomycin, two isolates (5.7%) showed intermediate susceptibility to neomycin, and another (2.9%) had intermediate susceptibility to tetracycline. This is the first report of isolation and antimicrobial susceptibilities of non‐typhoidal Salmonella serovars from dogs in Grenada. This study shows that dogs in Grenada may be involved in the epidemiology of salmonellosis.

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Laboratory surveillance of Salmonella enterica from human clinical cases in Colombia 2005–2011

Cited by 17 publications

References 32 publications

Salmonella isolates from urine cultures: serotypes and antimicrobial resistance in hospital settings

Salmonella isolates from urine cultures: serotypes and antimicrobial resistance in hospital settings

Salmonella enterica serovars Panama and Arechavaleta: Risk Factors for Invasive Non-Typhoidal Salmonella Disease in Guadeloupe, French West Indies

Serovars and antimicrobial resistance of non‐typhoidal Salmonella isolated from non‐diarrhoeic dogs in Grenada, West Indies

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