Laboratory-synthesized biogenic silver nanoparticles (AgNPs) with potential therapeutic applications

Abstract

This research was planned to isolate a marine bacterium that is able to biosynthesize silver nanoparticles to be used for therapeutic applications. The environmentally friendly production of nanoparticles from biological sources is frequently simple, inexpensive, and free of hazardous chemicals. Silver nanoparticles (AgNPs) are part of a new class of biomaterials that is being created more and more for use in scientific and medical endeavours. Given how important the biological system is, it is essential to have a basic understanding of how inorganic nanoparticles affect cellular development and function. Resazurin, a rapid screening assay, was applied to evaluate the antibacterial effects of the synthesized AgNPs against the pathogenic microbes. The 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay was utilized to test silver nanoparticles for cell cytotoxicity and anticancer activity. Silver nanoparticles (AgNPs) were tested for wound healing effects using a scratch assay on a human epithelial cell line (WISH-CCL-25). Halomonas sp. FSSH, among many isolates successfully biosynthesized silver nanoparticles with interesting properties. The present finding revealed that supernatant of Halomonas sp. FSSH could be effectively used as a reducing agent for the green production of AgNPs. These AgNPs are considered efficient antimicrobial, anticancer, and wound-healing agents without cytotoxic effects.