Laboratory Testing for Endocrine and Metabolic Disorders

Frank, Nicholas

doi:10.1002/9781118922798.ch60

Cited by 2 publications

(5 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2d,e) 34 . Insulin concentration above 45 μU/ml after 60 and 90 min was used as a threshold for insulin dysregulation 35 . No NW mares, six of seven OB mares (86%), and two of seven OBD mares (28%) were positive for insulin dysregulation (p = 0.005, Fig.…”

Section: Mare Morphometric Measurementsmentioning

confidence: 99%

“…For insulin measurements, blood was allowed to clot for 2 h at 4 °C, centrifuged at 1500 g for 10 min, and plasma was stored at − 20 °C until radioimmunoassays for insulin quantification by a reference lab (Endocrinology Lab, Cornell University Animal Health Diagnostic Center, Ithaca, NY). Insulin concentrations at either 60-or 90-min timepoints that were greater than 45 µU/mL were considered indicative of insulin dysregulation 35 . Insulin and glucose values collected prior to sugar administration were used to calculate the reciprocal of the square root of insulin [RISQI; (fasted insulin) -0.5 ] and the modified insulin-to-glucose ratio [MIRG; 800 − 0.3 (fasted insulin − 50) 2 / (fasted glucose − 30)], with the proxies estimating insulin sensitivity and pancreatic response to glucose, respectively 69 .…”

Section: Morphometric and Insulin Dysfunction Assessmentsmentioning

confidence: 99%

See 1 more Smart Citation

Adiposity in mares induces insulin dysregulation and mitochondrial dysfunction which can be mitigated by nutritional intervention

Fresa,

Catandi,

Whitcomb

et al. 2024

Sci Rep

View full text Add to dashboard Cite

Obesity is a complex disease associated with augmented risk of metabolic disorder development and cellular dysfunction in various species. The goal of the present study was to investigate the impacts of obesity on the metabolic health of old mares as well as test the ability of diet supplementation with either a complex blend of nutrients designed to improve equine metabolism and gastrointestinal health or L-carnitine alone to mitigate negative effects of obesity. Mares (n = 19, 17.9 ± 3.7 years) were placed into one of three group: normal-weight (NW, n = 6), obese (OB, n = 7) or obese fed a complex diet supplement for 12 weeks (OBD, n = 6). After 12 weeks and completion of sample collections, OB mares received L-carnitine alone for an additional 6 weeks. Obesity in mares was significantly associated with insulin dysregulation, reduced muscle mitochondrial function, and decreased skeletal muscle oxidative capacity with greater ROS production when compared to NW. Obese mares fed the complex diet supplement had better insulin sensivity, greater cell lipid metabolism, and higher muscle oxidative capacity with reduced ROS production than OB. L-carnitine supplementation alone did not significantly alter insulin signaling, but improved lipid metabolism and muscle oxidative capacity with reduced ROS. In conclusion, obesity is associated with insulin dysregulation and altered skeletal muscle metabolism in older mares. However, dietary interventions are an effective strategy to improve metabolic status and skeletal muscle mitochondrial function in older mares.

show abstract

Section: Mare Morphometric Measurementsmentioning

confidence: 99%

Section: Morphometric and Insulin Dysfunction Assessmentsmentioning

confidence: 99%

Adiposity in mares induces insulin dysregulation and mitochondrial dysfunction which can be mitigated by nutritional intervention

Fresa,

Catandi,

Whitcomb

et al. 2024

Sci Rep

View full text Add to dashboard Cite

show abstract

“…12,17 Nutritional secondary hyperparathyroidism is primarily differentiated from primary disease through dietary history and blood calcium and phosphorus concentrations. 7,13…”

mentioning

confidence: 99%

“…1,12,17 “Big head disease” or “bran disease” in horses has been associated with feedstuffs high in phosphorus and low in calcium, such as bran and sweet feed. 7,13 Diets with phosphorus concentrations at least double that of calcium predispose horses to this disease. 13,14 It is important that the basis of the equine diet be good quality hay; exclusively grain diets lead to improper calcium intake, with reported Ca:P ratios as low as 1:2.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Fibrous osteodystrophy in a dromedary camel

et al. 2020

View full text Add to dashboard Cite

A 6-y-old female dromedary camel ( Camelus dromedarius L.) was presented for assessment of firm, bilateral swellings rostral and ventral to the eyes. Serum biochemistry revealed hyperglycemia (28.5 mmol/L), hypocalcemia (1.27 mmol/L), hyperphosphatemia (3.39 mmol/L), hypoproteinemia (total protein 50 g/L), and hypoalbuminemia (20 g/L). Based on the poor prognosis associated with the presumptive diagnosis of fibrous osteodystrophy, the camel was euthanized. Gross postmortem findings revealed expanded fibrous tissue replacing the maxilla and mandible, and bilaterally prominent parathyroid glands. Histology of the maxilla revealed proliferative loose fibrous tissue with widely scattered, regularly spaced, small spicules of mineralized bone. The parathyroid glands were prominent bilaterally; the internal and external parathyroid glands were composed of plump cells with abundant pale basophilic cytoplasm and open nuclei. The pathologic findings were consistent with the antemortem diagnosis of fibrous osteodystrophy. The camel’s diet, which was not specifically balanced for a camel, included grass hay, sweet feed, and alfalfa pellets. The camel’s caregivers reported feeding her treats of cookies. A feed analysis was not available. The biochemistry abnormalities and clinical and postmortem findings, along with a diet that was not balanced for a camel, are consistent with a diagnosis of nutritional secondary hyperparathyroidism.

show abstract

Laboratory Testing for Endocrine and Metabolic Disorders

Cited by 2 publications

References 28 publications

Adiposity in mares induces insulin dysregulation and mitochondrial dysfunction which can be mitigated by nutritional intervention

Adiposity in mares induces insulin dysregulation and mitochondrial dysfunction which can be mitigated by nutritional intervention

Fibrous osteodystrophy in a dromedary camel

Contact Info

Product

Resources

About