Laboratory validation of a new gas-enhanced dentine liquid permeation evaluation system

Abstract

AIM: To validate a new automated dentine permeability testing platform based on pressure change measurements. METHODOLOGY: A split chamber was designed allowing for concomitant measurement of fluid permeation and pressure difference. In a first test, system reliability was assessed by interposing a solid metal disk, embedded composite resin disks, or teeth by consecutively measuring eight times under standardized conditions. Secondly, the repeatability and applicability of the method was tested in a dentine wound model by using intact third molars: Class I (2 × 5 mm) and a full occlusal preparation as well a ceramic restoration were consecutively performed and repeatedly measured eight times each. In the last test, the system detection limit as well correlation between gas pressure difference and liquid permeation were evaluated: Again, third molars were used and occlusal preparations of increasing size (2 × 5, 3 × 5, 4 × 5, and 5 × 5 mm and full occlusal preparations, respectively) were made. Data was analyzed for the linearity of measurement, and R (2) values were calculated. RESULTS: The embedding procedure allowed for perfect separation of the two chambers, and no significant variation in repeated measurements of evaluated samples for the respective treatments (p = 0.05) was found. The detection was 0.002 hPa/min for the pressure slope and 0.0225 l/min for the fluid infiltration, respectively. The saline volume was highly correlating to the gas pressure changes (R (2) = 0.996, p < 0.0001). CONCLUSIONS: The presented method is a reliable and exact tool to assess dentine permeability by nondestructive and repeatable measurements. CLINICAL RELEVANCE: This method is suitable for measurements and comparison of the effectiveness of dentine wounds sealing materials. Laboratory validation of a new gas-enhanced dentine liquid permeation evaluation systemAbstract Aim: To validate a new automated dentine permeability testing platform based on pressure change measurements.Methodology: A split-chamber was designed allowing for concomitant measurement of fluid permeation and pressure difference. In a first test, system reliability was assessed by interposing a solid metal disc, embedded composite resin discs, or teeth by consecutively measuring eight times under standardized conditions. Secondly, the repeatability and applicability of the method was tested in a dentine wound model by using intact third molars: Class I (2×5mm) and a full occlusal preparation as well a ceramic restoration were consecutively performed and repeatedly measured 8 times each. In the last test, the system detection limit as well correlation between gas pressure difference and liquid permeation were evaluated: Again, third molars were used and occlusal preparations of increasing size (2×5,3×5,4×5,5×5mm and full occlusal preparations, respectively) were made. Data was analyzed for the linearity of measurement and R 2 values were calculated. Results:The embedding procedure allowed for perfect separation of the two chambers and no signific...

Impact of Dynamic Loading on the Implant-abutment Interface Using a Gas-enhanced Permeation Test In Vitro

“…Two additional implants were used as negative controls without drilling a connection between the two chambers, thus serving to control the adequate embedding set-up. All implants were mounted and static GEPT values were assessed according to a previously published and validated protocol [ 5 , 9 ].…”