Laccase aggregates via poly-lysine-supported immobilization onto PEGA resin, with efficient activity and high operational stability and can be used to degrade endocrine-disrupting chemicals

Abstract

Immobilized enzymes possess catalytic activity as well as substrate specificity. Even though enzyme immobilization techniques for industrial applications are well established, the operational stability of immobilized enzymes often needs to...

Cascade chiral amine synthesis catalyzed by site-specifically co-immobilized alcohol and amine dehydrogenases

“…Enzyme immobilization is the most important strategy to address these problems. [14][15][16][17][18] Many efforts have been made to improve the catalysis performance of ADH and AmDH by developing efficient enzyme immobilization technology. 8,19,20 For example, Schmidt-Dannert et al designed a protein scaffold to site-specifically anchor ADH and AmDH with the aid of SpyTag-SpyCatcher for the production of (R)-2aminohexane.…”

Bioremediation of Hazardous Pollutants Using Enzyme-Immobilized Reactors

“…Because immobilized enzymes prepared by adsorptive, ionic, or covalent binding are immobilized at one or several points on the support materials, these immobilized enzymes have a high degree of freedom in their conformational structures and can only be reused a limited number of times, as they are easily desaturated and deactivated. To overcome this, complexes of enzymes and poly-Lys have been cross-linked and conjugated to a support material [46]. This method can immobilize the enzyme at high concentrations, thus improving catalyst efficiency.…”

Immobilized‐laccase bioreactors for wastewater treatment

“…Yamaguchi and Miyazaki (2021) studied the removal of the endocrine disruptor bisphenol A (BPA) (100 μM) by laccase immobilized by cross‐linking on polyethylene glycol acrylamide (PEGA) resin in batch and flow reactors. [ 23 ] They found a BPA removal of 144 μM h −1 at 30°C for the batch reactor and of 2880 μM h −1 at 50°C for the flow reactor. However, the volume of the batch reactor was not mentioned and that of the flow reactor was very tiny (a polytetrafluoroethylene tube of 39.25 μL).…”