Laccase-based biosensors for detection of phenolic compounds

Abstract

Monitoring of phenolic compounds in the food industry and for environmental and medical applications has become more relevant in recent years. Conventional methods for detection and quantification of these compounds, such as spectrophotometry and chromatography, are time consuming and expensive. However, laccase biosensors represent a fast method for on-line and in situ monitoring of these compounds. We discuss the main transduction principles. We divide the electrochemical principle into amperometric, voltammetric, potentiometric and conductometric sensors. We divide optical transducers into fluorescence and absorption. The amperometric transducer method is the most widely studied and used for laccase biosensors. Optical biosensors present higher sensitivity than the other biosensors. Laccase production is dominated by a few fungus genera: Trametes, Aspergillus, and Ganoderma. We present an overview of laccase biosensors used for the determination of phenolic compounds in industrial application

Versatile Electrochemical Platform for the Determination of Phenol‐like Compounds Based on Laccases from Different Origins

“…Laccase enzymes are widespread among plants,f ungi and bacteria.T hey catalyzet he oxidation of variousa romatic compounds as neurotransmitters [7],p harmaceuticals [24][25][26],h alogenated pesticides [27] and wastewaters from industrial processes [11,23].T his plethora of substrates have prompted laccases to emerge as promising recognition elements for the construction of sensors with diverse applications [28].W ec onsidert hat the unification of the way in which different laccases can be immobilized on as ensor surface is ar esult of technological relevance. Thep latform presented in this work is based on screen printing electrodes and its modification with silica nanospheres with ap Ia round 2 [ 29] allows the adsorption of fungal and plant laccases,d ue to their isoelectric points (pI) rangingf rom 3t o9 [ 30].T he approacht aken in this work is similar to the one usedf or the fabrication of glucose meters,acombination of screen printing with noncontact dispensing technologies [31,32].A lso,t he measurement procedure,c hronoamperometry,i st he same.…”

“…Thep latform presented in this work is based on screen printing electrodes and its modification with silica nanospheres with ap Ia round 2 [ 29] allows the adsorption of fungal and plant laccases,d ue to their isoelectric points (pI) rangingf rom 3t o9 [ 30].T he approacht aken in this work is similar to the one usedf or the fabrication of glucose meters,acombination of screen printing with noncontact dispensing technologies [31,32].A lso,t he measurement procedure,c hronoamperometry,i st he same. Therefore,i ts hould be possible to adapt ag reat number of the tests already presented in the literature to this format, avoidingt he use of moree xpensive surfaces as glassy carbon or gold, or more chemicald emanding procedures,l ike covalent or crosslinking bonding,r ecently reviewed by Parra and co-workers [28].…”

Electrosynthesized Molecularly Imprinted Polymer for Laccase Using the Inactivated Enzyme as the Target

“…They catalyze the oxidation of various organic substances such as o ‐ and p ‐diphenols, polyphenols, aminophenols, lignin, and inorganic ions with the concomitant reduction of molecular oxygen to water . They are widely used in a variety of industrial branches including paper, textile, food, cosmetics, but also as recognition element in biosensors for phenolic compounds . Since they were the first enzymes which effectively catalyze the cathodic reduction of dissolved oxygen at (carbon) electrodes they have been frequently applied as biocatalyst for oxygen reduction in biofuel cells …”

Statistical optimization for enhanced production of extracellular laccase from Aspergillus sp. HB_RZ4 isolated from bark scrapping

“…Among the various fungi, white rot fungi are the well-known and potent producers of laccase. In recent years laccase have attracted commercial significance due to their extensive use in food (Minussi et al 2002;Rodrìguez and Toca Herrera 2006), textile (Abadulla et al 2000), pulp and paper industry, waste water treatment (Casa et al 2003), bioremediation (Duran and Esposito 2000), nano-biotechnology, agrobiotechnology and pharmaceutical industries (Rodríguez-Delgado et al 2015;Legerská et al 2016). Laccase can be produced by batch, fed-batch or continuous cultivation.…”