Laccase-catalyzed cross-linking of BSA mediated by tyrosine

Abstract

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Comparative Mucomic Analysis of Three Functionally DistinctCornu aspersumSecretions

“…43 Additionally, a laccase, Laccase1, was identified, which catalyzes the oxidation and crosslinking of phenolic compounds. 38 Thus, tyrosinases, laccases, and other phenoloxidases may increase snail mucus integrity by crosslinking phenols of proteins and metabolites, similar to mechanisms used in other mollusks. 44 No proteins strongly identifying with P. viridis mussel foot glue proteins were identified in this study, though Fibrillin1 and Fibrillin2 showed some sequence similarity to mussel foot proteins.…”

“…29 Several enzymes that were found are involved in protein crosslinking, mucus network formation, or constructing biological glues, and likely serve a similar role in C. aspersum mucus. 38,39 Cysteines are abundant in mucins, and disulfide isomerases, like the identified DisulfideIsomerase1, construct mucus gels by catalyzing interchain disulfide bonds. 39 A prolyl isomerase, ProlylIsomerase1, was found, which has signaling and immune functions in mucus, 40 and this class of proteins also regulates collagen crosslinking.…”

Ultrasensitive Detection of Aqueous Uranyl Based on Uranyl‐Triggered Protein Photocleavage

“…The combination of these two protein fragments amounted to the molecular weight of BSA (66 445 Da) and a molecule of water, indicating that cleavage occurs at specific site in BSA by hydrolysis of the protein. An additional peak was also detected in the BSA spectrum, which is corresponding to the protein BSA charged with two H + [20] . However, as the irradiation time extended from 15 min to 30 min, additional protein bands were observed due to the nonspecific shear of the initial protein BSA and the newly generated protein fragments (Supporting Information, Figure S6).…”

“…An additional peak was also detected in the BSA spectrum, which is corresponding to the protein BSA charged with two H + . [20] However, as the irradiation time extended from 15 min to 30 min, additional protein bands were observed due to the nonspecific shear of the initial protein BSA and the newly generated protein fragments (Supporting Information, Figure S6). Moreover, the change in fluorescence intensity was tested along with the last of the irradiation time and the result obtained through the fitting curve indicated that the time point for the fastest fluorescence attenuation occurs at approximately 12.13 minutes (Supporting Information, Figure S7).…”

Studies of the Effect of Electromagnetic Radiation on Biosensor Matrix Components

“…Do badań wybrano białko albuminy surowicy bydlęcej BSA (ang. Bovine Serum Albumin), które jest powszechnie stosowane w warstwach receptorowych biosensorów podczas sieciowania [16] oraz jego kompleksy BSA-Au, BSA-NAA.…”