Neurochemicals are a group of small organic molecules or peptides that influence the functioning of brain activity. An imbalance in neurochemicals poses a threat to human health, making their detection and determination in human biological fluids important for diagnosis and treatment. Electrochemical biosensors have emerged as selective, sensitive, and reliable tools for detecting and determining neurochemicals. Furthermore, incorporating nanomaterials onto the surface of the working electrode has further improved the detection capabilities of these biosensors. Hence, this chapter summarizes the development of aptamers and enzyme biosensors for the electrochemical detection or determination of neurochemicals over the last two years.