Laccase production and metabolic diversity among Flammulina velutipes strains

Janusz, Grzegorz; Czuryło, Aleksandra; Frąc, Magdalena; Rola, Beata; Sulej, Justyna; Pawlik, Anna; Siwulski, Marek; Rogalski, Jerzy

doi:10.1007/s11274-014-1769-y

Cited by 44 publications

(44 citation statements)

References 58 publications

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“…A similar result was obtained in Flammulina velutipes. [28] In this study, we screened the laccase inducers in P. ostreatus strain ACCC 52857 and examined the effects of the inducers and their timedependent addition.…”

Section: Introductionmentioning

confidence: 99%

Optimization of laccase production in the white-rot fungusPleurotus ostreatus(ACCC 52857) induced through yeast extract and copper

Zhu

Bao

Huang

2016

Biotechnology & Biotechnological Equipment

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Different inducers for laccase production in Pleurotus ostreatus (ACCC 52857) were screened: carbon and nitrogen source, phenolic compounds and metal ions. Among the tested substances, yeast extract and copper showed the strongest effect on laccase activity. Laccase activity increased during the early phase of cultivation in the presence of yeast extract, peaking on the 6th day and decreasing thereafter. Copper-induced laccase activity increased both in a dose-dependent and a time-dependent manner. The highest laccase activity was obtained with 2 mmol/L Cu 2C , while the mycelial growth was inhibited approximately 27%. Thus, the time-dependent effect of copper on laccase activity was examined. The results showed that the best laccase production was induced when copper was added during the mid-logarithmic phase of cultivation (the 5th day). A positive synergistic effect of yeast extract and copper on the laccase production was observed. Laccase activity dramatically increased upon the addition of copper to medium containing 1% yeast extract on the 5th day of cultivation. The highest activity (8533.33 § 1228.94 U/mL) was observed on the 13th day of cultivation, increased more than 80 folds compared to the original level.

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Section: Introductionmentioning

confidence: 99%

Optimization of laccase production in the white-rot fungusPleurotus ostreatus(ACCC 52857) induced through yeast extract and copper

Zhu

Bao

Huang

2016

Biotechnology & Biotechnological Equipment

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“…To the best of our knowledge, there are no reports describing the genetic and functional diversities of Petriella setifera through AFLP fingerprinting and BIOLOG FF Plates™. For the last 15 years many researchers used the AFLP analysis to identify the intraspecific variability within a fungal species (Bakkeren, Kronstad & Lévesque, 2000;Tooley et al, 2000;Abdel-Satar et al, 2003;Radišek et al, 2003;Schmidt, Niessen & Vogel, 2004;Perrone et al, 2006a,b, Pawlik et al, 2015aRola et al, 2015), and 7 years ago they began to use the methodology BIOLOG to estimate the functional diversity (Feng et al, 2009;Singh, 2009;Albrecht et al, 2010;Shengnan et al, 2011;Lucas et al, 2013;Janusz et al, 2015;Pawlik et al, 2015a,b;Rola et al, 2015;Panek, Frąc & Bilińska-Wielgus, 2016). All these investigations explain the validity and suitability of using these methodologies to discover the intraspecific differences between fungal species at the genetic and functional level.…”

Section: Discussionmentioning

confidence: 99%

“…These indices are especially sensitive indicators that reveal the differences between the strains (Frąc, Oszust & Lipiec, 2012). In the last year, the BIOLOG system was introduced for rapid characterization of the fungal community (Stefanowicz, 2006;Singh, 2009;Janusz et al, 2015;Pawlik et al, 2015a,b;Rola et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Intraspecific functional and genetic diversity of Petriella setifera

Pertile

Panek

Oszust

et al. 2017

Preprint

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Petriella setifera is poorly-known species with only fragmentary information in literature and with published partial genome about the fungal species. Therefore, the aim of the study was an analysis of the intraspecific genetic and functional diversity of new isolated fungal species of P. setifera. From the molecular biological viewpoint, the intraspecific variability was found through the Amplified Fragment Length Polymorphism analysis (AFLP). The analysis showed a good variability among the analysed isolates, which was demonstrated by the clear subdivision of all the isolates into two clusters with 51% and 62% of similarity, respectively. For the metabolic diversity, the BIOLOG system was used and this analysis revealed clear different patterns on the carbon substrates utilization between the isolates bringing a clear separation of the five isolates into three clusters with 0%, 42% and 54% of similarity, respectively. These two techniques allowed estimation of the intraspecific variability within the five isolates of P. setifera strains. Both the methodologies are two easy and rapid techniques to indicate the genetic and functional variability at the intraspecific level within the species, especially, if a biological and functional information about the analysed fungal strains are limited.

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“…However, these differences were determined using mycelial cultures and no more data was derived from mushroom growing in substrates. Moreover, no comparison between cultivated and wild strains has been given [26].…”

Section: Discussionmentioning

confidence: 99%

A Comparative Study on Bioconversion of Different Agro Wastes by Wild and Cultivated Strains of Flammulina velutipes

Rezaeian

Pourianfar

2016

Waste Biomass Valor

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Purpose Little knowledge is available regarding the possibility of conversion of agro wastes through cultivation of indigenous wild strains of Flammulina velutipes and interactions between substrate composition and F. velutipes strain. This study was thus conducted to compare effects of several locally available agro wastes on the growth characteristics of Iranian wild and cultivated strains of F. velutipes. Methods Nine different substrate compositions were made based on sawdust, sugarcane bagasse, soybean meal, wheat bran, and wheat straw. The main and simple effects of the substrate compositions as well as interactions between mushroom strain and substrate were comparatively determined. Results While significant interactions between substrate and mushroom strain were observed (p \ 0.05), the mixtures of wheat straw and wheat bran enriched with soybean meal or sawdust highly supported vegetative and reproductive growth of both strains (p \ 0.05). Amongst the selected formulations that generated biological efficiencies more than 100 %, the substrate containing 40 % wheat straw ? 40 % sawdust ? 18 % wheat bran gave an outstanding biological efficiency of 265 % for the cultivated strain (p \ 0.05). Amid the serious environmental problems caused by the accumulation of sugarcane bagasse in Iran, this study also showed that both wild and cultivated F. velutipes can effectively be grown on sugarcane bagasse supplemented with wheat bran. Conclusion The selected agricultural wastes evaluated in this study may be suggested as new formulations for growing either indigenous or cultivated strains of medicinal mushroom F. velutipes.

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Laccase production and metabolic diversity among Flammulina velutipes strains

Cited by 44 publications

References 58 publications

Optimization of laccase production in the white-rot fungusPleurotus ostreatus(ACCC 52857) induced through yeast extract and copper

Optimization of laccase production in the white-rot fungusPleurotus ostreatus(ACCC 52857) induced through yeast extract and copper

Intraspecific functional and genetic diversity of Petriella setifera

A Comparative Study on Bioconversion of Different Agro Wastes by Wild and Cultivated Strains of Flammulina velutipes

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